Apoptosis, proliferation, and angiogenesis in oral tissues. Possible relevance to tumour progression

Macluskey, Michaelina; Chandrachud, Lata M.; Pazouki, Sima; Green, Michael; Chisholm, D. M.; Ogden, Graham S.; Schor, Seth L.; Schor, Ana M.

doi:10.1002/1096-9896(2000)9999:9999<::aid-path652>3.3.co;2-p

Cited by 22 publications

(23 citation statements)

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“…Furthermore, we found significant correlations of survivin expression with histological typing, clinical staging and lymphnode metastasis, suggesting survivin is important in TC evolution and a poor prognostic molecular indicator for TC. Imbalance between proliferation and apoptosis has been proposed as one of key pathogenic mechanisms in tumor [18][19][20][21]. Caspase-3 is one of the most specific proteases involved in the apoptosis mechanism.…”

Section: Discussionmentioning

confidence: 99%

Significance of survivin, caspase-3, and VEGF expression in thyroid carcinoma

Zhang

Meng

et al. 2009

Clin Exp Med

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To investigate the clinical significance of survivin, caspase-3, and vascular endothelial growth factor expression in a subset of thyroid carcinoma and their correlation with prognosis. Sixty-eight cases of thyroid carcinoma (TC), 12 cases of thyroid adenoma (TA) and 10 cases of normal thyroid tissue (NT) were involved in immunohistochemical and real-time RT-PCR analyses for survivin, caspase-3, and VEGF expression. Statistical analyses were performed for differential expression among NT, TA and TC, correlations of their expression with the clinicopathological parameters of TC including histological typing, clinical staging and lymphnode metastasis, and relationship of survivin with caspase-3 or VEGF in TC. We observed higher mRNA expression and positive immunostaining for survivin and VEGF in TC compared with TA and NT, with a significant positive correlation among them and significant correlations with histological typing, clinical staging and lymphnode metastasis in TC, but we could not find any significance of caspase-3 in TC and its significant relationship with survivin expression. Our results indicate that survivin and VEGF are unfavorable molecules for TC evolution and prognosis, and possess positive correlation in TC.

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Section: Discussionmentioning

confidence: 99%

Significance of survivin, caspase-3, and VEGF expression in thyroid carcinoma

Zhang

Meng

et al. 2009

Clin Exp Med

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“…TUNEL-positive cells appeared red and exhibited cell shrinkage, nuclear margination, chromatin condensation, and nucleolar disintegration, whereas TUNELnegative nuclei appeared blue. About 2,000 tumor cells were counted, and the count was expressed as a percentage of ''apoptotic'' positive cells (Macluskey et al 2000). One negative and one positive control were included in each experiment.…”

Section: Histopathology Experimentsmentioning

confidence: 99%

In vivo induction of necrosis in mice fibrosarcoma via intravenous injection of type B staphylococcal enterotoxin

et al. 2008

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The bacterial superantigen staphylococcal enterotoxin B (SEB) is a potent inducer of cytotoxic T-cell activity and cytokine production in vivo. We investigated the possibility of the therapeutic application of SEB in patients with fibrosarcoma. The anti-tumor effect of SEB in mice with inoculated fibrosarcoma (WEHI-164) was examined by intravenous (IV) and intratumoral (IT) injection and the sizes of the inoculated tumors, IFN-gamma production, and CD4+/CD8+ T cell infiltration were determined. The inoculated tumors were also examined histologically. In the mice in the IV-injected group, a significant reduction (P < 0.02) of tumor size was observed in comparison with mice in the IT-injected and control groups. Furthermore, the mice in the IV-injected group showed significantly higher levels of IFN-gamma (P < 0.009) and CD4+/CD8+ T cell infiltration when compared with the other groups (P < 0.02). A significantly higher frequency of necrosis in tumor tissues was also observed in mice in the IV-injected group (P < 0.05). Our present findings suggest that tumor cell death is caused by increased cytotoxic T-cell activity and cytokine levels in response to the IV injection of SEB and that SEB may be a good option for use as a novel therapy in patients with fibrosarcoma.

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“…A subset of these lesions, referred to as angiogenic squamous dysplasias (ASDs), also show distinctive morphological features of angiogenic activity with projections of capillary loops into overlying dysplastic epithelium [21]. A number of publications, primarily employing qualitative immunohistochemical (IHC) and in situ hybridization analyses of tissue sections from premalignant lesions of the lung, cervix, breast, colon and head and neck regions, have suggested that overexpression of VEGF could be a relatively early event in the progression to malignancy at these sites [23][24][25][26][27]. It is difficult to gauge the degree of VEGF overexpression from these studies, and it has been noted that differences in technique can lead to very different conclusions in studies employing such procedures [28].…”

Section: Introductionmentioning

confidence: 99%