Apoptosis of T-leukemia and B-myeloma cancer cells induced by hyperbaric oxygen increased phosphorylation of p38 MAPK

Chen, Yen‐Chen; Chen, Shao-Yuan; Ho, Pey-Shen; Lin, Chia-Huei; Cheng, Ya-Yuan; Wang, Jehng-Kang; Sytwu, Huey‐Kang

doi:10.1016/j.leukres.2006.09.016

Cited by 27 publications

(26 citation statements)

References 22 publications

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“…More specifically, tumor cells are destroyed if ROS level rises beyond the limit of a tumor's resistance to oxidative stress following treatment with HBO. In fact, several recently published animal studies found that exposure to HBO alone induced tumor cell apoptosis (30)(31)(32). In the present study as well, DNA ladder formation was seen 48 h after treatment with HBO, while none was observed in the group without HBO treatment.…”

Section: Discussionsupporting

confidence: 75%

Hyperbaric oxygen as a chemotherapy adjuvant in the treatment of osteosarcoma

Kawasoe

Yokouchi²,

Ueno³

et al. 2009

Oncol Rep

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Abstract. Although hyperbaric oxygen has been shown to enhance the efficacy of radiotherapy and chemotherapy for the treatment of several malignant tumors, the impact of hyperbaric oxygen on osteosarcoma has not yet been demonstrated. In this study, we investigated the efficacy of hyperbaric oxygen alone and in combination with an anticancer drug as an adjuvant to chemotherapy. In vitro, highly metastatic murine osteosarcoma cell lines were exposed to hyperbaric oxygen and cell viability was examined. Hyperbaric oxygen alone significantly suppressed cell proliferation, and hyperbaric oxygen plus carboplatin exhibited significant synergism in suppression of cell proliferation. In vivo, C3H mice were subcutaneously inoculated with osteosarcoma cells and divided into four groups: control, hyperbaric oxygen, carboplatin, and carboplatin plus hyperbaric oxygen. After 5 weeks, increase in both tumor volume and number of lung metastases was significantly suppressed in the hyperbaric oxygen group. Concomitant hyperbaric oxygen clearly enhanced the chemotherapeutic effects of carboplatin on both tumor growth and lung metastasis in osteosarcoma-bearing mice. Moreover, mortality in the carboplatin plus hyperbaric oxygen group was significantly lower than in the other three groups. These findings suggest that hyperbaric oxygen plus carboplatin combination therapy could be an appropriate therapeutic regimen for the treatment of patients with osteosarcoma.

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Section: Discussionsupporting

confidence: 75%

Hyperbaric oxygen as a chemotherapy adjuvant in the treatment of osteosarcoma

Kawasoe

Yokouchi²,

Ueno³

et al. 2009

Oncol Rep

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“…However, the effect of hyperbaric oxygen treatment alone on tumor treatment remains controversial (21). Some studies suggested that HBO may possess tumor-inhibitory effects (22)(23)(24)(25), while others indicated that HBO treatment alone may stimulate tumor growth and metastasize (8,26,27).…”

Section: Discussionmentioning

confidence: 99%

Effects of hyperbaric oxygen treatment on gastric cancer cell line SGC7901

Ruan

Wang

et al. 2017

Biomedical Reports

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Abstract. Hyperbaric oxygen (HBO) has been previously identified as an effective adjunct treatment option for the management of brain injury, diabetic ulcers and chronic wounds. However, the roles of HBO as an adjunctive therapy for tumors remain controversial. The present research project was performed to explore the effects of HBO treatment on proliferation, autophagy and endoplasmic reticulum stress response of the gastric cancer cell line, SGC7901. The present study demonstrated that, after subjecting SGC7901 cells to HBO treatment, the increase in cell proliferation was significant, compared with that of the control group. In addition, there was a significant increase in LC3-phosphatidylethanolamine conjugate (LC3-II) level, as well as binding immunoglobulin protein level, and a significant decrease in CCAAT-enhancer-binding protein homologous protein level. These suggested that hyperbaric oxygen treatment alone may promote proliferation and cell survival of gastric cancer cell SGC7901, and inhibit apoptosis through regulating cell autophagy and oxidative stress. IntroductionWidely used in the clinic, hyperbaric oxygen (HBO) treatment has be shown to be an effective adjunct to management of brain injury (1), diabetic ulcers (2) and chronic wounds (3). However, the roles of HBO as an adjunctive therapy for tumors remains controversial. Some previous studies suggest that HBO therapy may improve postoperative outcome and outcome of radiotherapy and chemotherapy, especially proving beneficial for the treatment of radiotherapy (4,5). A combinatorial approach using chemotherapeutic drugs with HBO may enhance sensitivity of tumor cells to chemotherapeutic agents (6,7) and the application of HBO in chemotherapy for malignant lymphoma, brain tumor, lung cancer, gastric cancer and breast cancer may increase chemotherapeutic efficacy while decreasing treatment related toxicity (8). However, HBO treatment alone may stimulate the proliferation of tumor tissues (9). Other studies indicate that cancer cells in hypoxic conditions are more likely to metastasize and thus be more deadly (10-12). Meanwhile, hypoxic cells undergo a high rate of mutation to become a treatment-resistant genotype, and HBO treatment may improve it (13). The current study is to explore the effects of hyperbaric oxygen treatment alone on proliferation, autophagy and oxidative stress response of gastric cancer SGC7901 cells. Materials and methodsCell culture. Gastric cancer SGC7901 cell lines were purchased from the American Type Culture Collection (Manassas, VA, USA), inoculated in Dulbecco's modified Eagle's (high glucose) culture medium (Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA) with 10% fetal bovine serum (Clark Bioscience, Richmond, VA, USA) and cultured in a 5% CO 2 incubator at 37˚C. HBO treatment.Prior to the experiment, the hyperbaric chamber was disinfected using UV techniques for 20 min, and cleaned using pure oxygen for 10 min. The cells were placed flat in the chamber under aseptic conditions for 90 min each time. The press...

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“…As an overall effect, at least in rodents, hyperbaric oxygenation appears to be antiinflammatory since it increases the susceptibility to respiratory infections [23,24] and delays allograft rejection [25][26][27]. While hyperbaric oxygenation may inhibit apoptosis in models of ischemic brain injury [28,29] and ischemic wound healing [30] possibly due to limiting tissue hypoxia, a single exposure to high partial oxygen pressure was observed to enhance apoptosis mouse fibroblasts [31], Jurkat-T-cells, HL-60 cells, murine thymocytes [32] and NCI-H929 [33].…”

Section: Introductionmentioning

confidence: 99%

Hyperbaric oxygen induces apoptosis via a mitochondrial mechanism

Weber

Koch²,

Kankeleit

et al. 2008

Apoptosis

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During therapeutic hyperbaric oxygenation lymphocytes are exposed to high partial pressures of oxygen. This study aimed to analyze the mechanism of apoptosis induction by hyperbaric oxygen. For intervals of 0.5-4 h Jurkat-T-cells were exposed to ambient air or oxygen atmospheres at 1-3 absolute atmospheres. Apoptosis was analyzed by phosphatidylserine externalization, caspase-3 activation and DNA-fragmentation using flow cytometry. Apoptosis was already induced after 30 min of hyperbaric oxygenation (HBO, P < 0.05). The death receptor Fas was downregulated. Inhibition of caspase-9 but not caspase-8 blocked apoptosis induction by HBO. Hyperbaric oxygen caused a loss of mitochondrial membrane potential and caspase-9 induction. The mitochondrial pro-survival protein Bcl-2 was upregulated, and antagonizing Bcl-2 function potentiated apoptosis induction by HBO. In conclusion, a single exposure to hyperbaric oxygenation induces lymphocyte apoptosis by a mitochondrial and not a Fas-related mechanism. Regulation of Fas and Bcl-2 may be regarded as protective measures of the cell in response to hyperbaric oxygen.

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Apoptosis of T-leukemia and B-myeloma cancer cells induced by hyperbaric oxygen increased phosphorylation of p38 MAPK

Cited by 27 publications

References 22 publications

Hyperbaric oxygen as a chemotherapy adjuvant in the treatment of osteosarcoma

Hyperbaric oxygen as a chemotherapy adjuvant in the treatment of osteosarcoma

Effects of hyperbaric oxygen treatment on gastric cancer cell line SGC7901

Hyperbaric oxygen induces apoptosis via a mitochondrial mechanism

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