Apoptosis of peripheral blood leukocytes from rabbits infected with non-haemagglutinating strains of rabbit haemorrhagic disease virus (RHDV)

Niedźwiedzka-Rystwej, Paulina; Deptuła, Wiesław

doi:10.1016/j.vetimm.2012.06.012

Cited by 23 publications

(41 citation statements)

References 11 publications

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“…In some cases, senescence may have an advantage over apoptosis as it allows communication between cells (Childs, Baker, Kirkland, Campisi, & van Deursen, ). Apoptosis of hepatocytes was found to be induced during RHD (Jung, Lee, Tai, Park, & Lee, ; Niedźwiedzka‐Rystwej & Deptuła, ), and the finding of significant overrepresentation of GO terms connected with cell ageing within our candidate genes suggests that this mechanism is important for surviving RHDV infection. The functions of the candidate genes with the lowest p ‐values annotated with the GO term “cellular senescence” (ABL1, CDKN1A and HMGA1) and their potential relevance in RHDV infections are summarized in Appendix .…”

Section: Discussionmentioning

confidence: 71%

Resistance to RHD virus in wild Australian rabbits: Comparison of susceptible and resistant individuals using a genomewide approach

et al. 2017

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Deciphering the genes involved in disease resistance is essential if we are to understand host-pathogen coevolutionary processes. The rabbit haemorrhagic disease virus (RHDV) was imported into Australia in 1995 as a biocontrol agent to manage one of the most successful and devastating invasive species, the European rabbit (Oryctolagus cuniculus). During the first outbreaks of the disease, RHDV caused mortality rates of up to 97%. Recently, however, increased genetic resistance to RHDV has been reported. Here, we have aimed to identify genomic differences between rabbits that survived a natural infection with RHDV and those that died in the field using a genomewide next-generation sequencing (NGS) approach. We detected 72 SNPs corresponding to 133 genes associated with survival of a RHD infection. Most of the identified genes have known functions in virus infections and replication, immune responses or apoptosis, or have previously been found to be regulated during RHD. Some of the genes identified in experimental studies, however, did not seem to play a role under natural selection regimes, highlighting the importance of field studies to complement the genomic background of wildlife diseases. Our study provides a set of candidate markers as a tool for the future scanning of wild rabbits for their resistance to RHDV. This is important both for wild rabbit populations in southern Europe where RHD is regarded as a serious problem decimating the prey of endangered predator species and for assessing the success of currently planned RHDV variant biocontrol releases in Australia.

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Section: Discussionmentioning

confidence: 71%

Resistance to RHD virus in wild Australian rabbits: Comparison of susceptible and resistant individuals using a genomewide approach

et al. 2017

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“…Apoptosis refers to many cells of the macro--organism, and occurs both in physiological and pathological conditions, and it is now assumed to be an important element of immunity of mammals (Niedźwiedzka-Rystwej and Deptuła 2012, Niedźwiedzka--Rystwej and Deptuła 2012a, Niedźwiedzka-Rystwej et al 2013, Niedźwiedzka-Rystwej et al 2013a. Its role in immunology is related to, among others, immune system cells, which, after performing their function, are thus eliminated as damaged or changed cells (Baś et al 2004, Elmore 2007, Niedźwiedzka--Rystwej and Deptuła 2012, Niedźwiedzka-Rystwej et al 2013, Niedźwiedzka-Rystwej et al 2013a.…”

Section: Introductionmentioning

confidence: 99%

“…Its role in immunology is related to, among others, immune system cells, which, after performing their function, are thus eliminated as damaged or changed cells (Baś et al 2004, Elmore 2007, Niedźwiedzka--Rystwej and Deptuła 2012, Niedźwiedzka-Rystwej et al 2013, Niedźwiedzka-Rystwej et al 2013a. The facts place apoptosis of immune system cells at the focus of interest among immunologists, including diagnostics specialists.…”

Section: Introductionmentioning

confidence: 99%

“…The facts place apoptosis of immune system cells at the focus of interest among immunologists, including diagnostics specialists. It has been shown that apoptosis of peripheral blood lymphocytes and granulocytes is a good prognostic factor used to assess immunity of the macro-organism (Weinmann et al 2003, Stark et al 2005, including in viral infections in humans with such viruses as EBV, HBV, HCV, HCMV, HPV and HSV (Solary et al 1996, O'Brien 1998, Fadeel et al 1999, as well as in rabbits infected with RHDV (Alonso et al 1998, Niedźwiedzka and Deptuła 2012, Niedźwiedzka and Deptuła 2012a, Hukowska--Szematowicz 2013, Niedźwiedzka at al. 2013, Niedźwiedzka et al 2013a.…”

Section: Introductionmentioning

confidence: 99%

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Values of apoptosis of lymphocytes and granulocytes in peripheral blood of Polish mixed-breed rabbits in the annual cycle

Tokarz–Deptuła

Hukowska-Szematowicz

Niedźwiedzka-Rystwej

et al. 2017

Polish Journal of Veterinary Sciences

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The objective of the study was to determine values of apoptosis for peripheral blood lymphocytes and granulocytes, including dependency on the season of the year, in Polish mixed-breed rabbits and in mixed-breed rabbits with the addition of blood of meat-breed rabbits. The study was carried out in four seasons of the year (spring, summer, autumn, winter) involving 120 Polish mixed-breed rabbits -group I, and 120 Polish mixed-breed rabbits with addition of meat-breed rabbit blood -group II. Assessment of apoptosis of lymphocytes and granulocytes was performed using as FACScan cytometer by Becton Dickinson with FACSDiva software (USA), using as ApoFluor ® Green Caspase reagent kit (MP Biomedicals, USA) to detect the activity of the total caspase pool 1, 3, 4, 5, 6, 7, 8, 9 in granulocytes and lymphocytes of rabbit peripheral blood. The results for apoptosis of lymphocytes and granulocytes in peripheral blood in the animals investigated (group I and II) were subjected to statistical analysis with the t'Student test at p=0.05. It was noted that, in rabbits from group I, the values for apoptosis of lymphocytes were the highest in winter and autumn (36.02% and 31.24%, respectively), and the lowest in spring and summer (26.73% and 22.72%, respectively), whereas in the case of granulocytes the highest values were in summer and spring (14.69% and 12.95%, respectively), and the lowest in winter and autumn (8.16% and 8.57%, respectively). In mixed-breed rabbits with the addition of meat-breed blood (group II), the values for apoptosis of lympocytes were the highest in spring (29.13%), and the lowest in summer (25.43%); whereas in the case of granulocytes the highest values were in summer and spring (14.0% and 11.15%, respectively), and the lowest in autumn and winter (7.46% and 7.64%, respectively).

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“…With time and the growing interest of researchers in the virus and the pathogenesis of the disease caused by it, the participation of other factors in the pathogenesis of the disease was considered. Further studies focused on the liver, where the virus causes the greatest anatomic and pathological lesions, and it turned out that, in this organ, intensified cell death processes occur by way of necrosis (Park et al 1995) and apoptosis (Alonso et al 1998); the latter of these processes is also related to peripheral blood leukocytes from rabbits infected with RHDV (Niedźwiedzka-Rystwej and Deptuła 2012a, b). Moreover, other examinations showed that, during rabbit infection with RHDV, there is overexpression of pro-inflammatory cytokines in the liver, which is probably one of the causes of pathological lesions in this organ (Tuñón et al 2011a, b).…”

Section: Introductionmentioning

confidence: 99%

Analysis of genetic variability and phylogenetic analysis of selected Czech and French strains of rabbit haemorrhagic disease virus (RHDV)

2013

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The objective of this study was to analyse the genetic variability and phylogenetic analysis of six strains of rabbit haemorrhagic disease virus (RHDV), including four Czech strains (CAMPV-351, CAMPV-561, CAMPV-562, CAMPV-558) and two French strains (Fr-1, Fr-2), on the basis of a fragment of the VP60 capsid structural protein-coding gene N-terminal region. The results of our own studies were compared to 26 RHDV strains obtained from GenBank. The analysis of the genetic variability of six RHDV strains indicated that the CAMPV-561 strain is the most genetically variable. Less variable were the Fr-1 and Fr-2 strains, while the least variable was CAMPV-351. In turn, the genetic distance among the six analysed strains and 26 strains obtained from GenBank was the greatest for CAMPV-351 and Whn/China [11.3 % according to the observed divergence (OD) method and 12.2 % according to the maximum likelihood (ML) method], while it was the lowest for CAMPV-351 and FRG (0.8 % in both the OD and ML methods). In turn, the scale of the genetic distances among the six analysed strains and five RHDVa strains (99-05, NY-01, Whn/China, Triptis, Iowa2000) ranged from 9.3–10.3 % in the OD method to 10.3–13.7 % in the ML method. The image of phylogenetic dependencies generated for the strains analysed and those obtained from GenBank revealed their distribution to be in five genetic groups (G1–G5), whereas the analysed strains were included in genetic groups 2 and 3.

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Apoptosis of peripheral blood leukocytes from rabbits infected with non-haemagglutinating strains of rabbit haemorrhagic disease virus (RHDV)

Cited by 23 publications

References 11 publications

Resistance to RHD virus in wild Australian rabbits: Comparison of susceptible and resistant individuals using a genomewide approach

Resistance to RHD virus in wild Australian rabbits: Comparison of susceptible and resistant individuals using a genomewide approach

Values of apoptosis of lymphocytes and granulocytes in peripheral blood of Polish mixed-breed rabbits in the annual cycle

Analysis of genetic variability and phylogenetic analysis of selected Czech and French strains of rabbit haemorrhagic disease virus (RHDV)

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