Apoptosis of periodontal ligament cells induced by mechanical stress during tooth movement

Hatai, Takiko; Yokozeki, Masahiko; Funato, Noriko; Baba, Yoshifumi; Moriyama, Keiji; Ichijo, Hidenori; Kuroda, Takayuki

doi:10.1034/j.1601-0825.2001.00663.x

Cited by 38 publications

(37 citation statements)

References 18 publications

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“…Hatai et al 19) reported that they applied mechanical stress during orthodontic tooth movement by using the 50 g nickel titanium and Mabuchi et al 20) reported using the Waldo method. It is believed that it varies in a tooth migration method and a movement power that a day of the expression is different from them.…”

Section: Discussionmentioning

confidence: 99%

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Effect of Compression Force on Apoptosis in Human Periodontal Ligament Cells

Funakoshi

Yamaguchi

Asano

et al. 2013

J. Hard Tissue Biology.

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The effect of compression force on periodontal ligament (PDL) plays a critical role in orthodontic tooth movement. However, little is known about this effect on apoptosis and the underlying reactive mechanisms in PDL cells. This study focuses on the application of compression force associated with reactive oxygen species (ROS)-induced apoptosis in PDL cells. Following the application of orthodontic force to maxillary first molars, the molars were investigated using immunohistochemical analysis. The cell cycle and apoptosis were assessed in compression force-treated PDL cells using flow cytometry. Furthermore, we assayed the effect on ROS generation by DCFDA fluorescence. In vivo study revealed an increased number of terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) and caspase 8-positive cells in the orthodontic force group. In our in vitro study model, the compression force increased the G1 phase and apoptotic cells. A large increase in the intracellular ROS levels in human (h) PDL cells was observed in the compression force group. Taken together, these results provide new information on compression force-induced G1 arrest and apoptosis in hPDL cells.

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Section: Discussionmentioning

confidence: 99%

“…1). During tooth movement in vivo, recent studies have reported that the number of TUNEL-positive periodontal ligament cells increases at 12 h and reaches a maximum at 24 h 19) . In addition, in vivo, the ratio of TUNEL-positive cells on the pressure side of the periodontal ligament increased up to day 28 20) .…”

Section: Discussionmentioning

confidence: 99%

Effect of Compression Force on Apoptosis in Human Periodontal Ligament Cells

Funakoshi

Yamaguchi

Asano

et al. 2013

J. Hard Tissue Biology.

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“…Esses animais oferecem algumas vantagens, dentre elas a manutenção com menor custo -quando comparados aos primatas, gatos e cães -e o emprego em larga escala, devido ao curto período de procriação e ninhadas com vários filhotes 27 . A biologia da movimentação dentária induzida tem seus aspectos morfológicos microscópicos repetidamente descritos em vários artigos, teses e livros 2,5,7,8,13,14,21,23,25,32,33,35,36 . Ao microscópio óptico, as modificações biológicas são observadas no ligamento periodontal, no osso alveolar e nas raízes dos dentes movimentados.…”

Section: Introductionunclassified

Movimentação dentária experimental em murinos: período de observação e plano dos cortes microscópicos

Fracalossi

Santamaria‐Jr

Consolaro³

et al. 2009

Rev. Dent. Press Ortodon. Ortop. Facial

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ResumoObjetivo: este trabalho tem por finalidade explicitar aspectos microscópicos relevantes da movimentação dentária induzida em murinos quanto aos: (1) diferentes períodos de observação e (2) planos dos cortes microscópicos transversais e longitudinais. Os estudos experimentais sobre a movimentação dentária induzida em murinos variam quanto aos períodos e planos de cortes microscópicos, mesmo os trabalhos que utilizam especificamente o modelo de Heller e Nanda, de 1979. Para contribuir (1) na escolha do melhor design experimental de movimentação dentária induzida em murinos em futuros trabalhos, e (2) no aperfeiçoamento dos critérios de análise por outros pesquisadores, propusemo-nos a publicar este artigo. Métodos: Empregaram-se 50 ratos machos Wistar, com 90 dias de vida, submetidos à movimentação dentária induzida por períodos de 3, 5, 7 e 9 dias. Utilizou-se movimento de inclinação mesial no primeiro molar superior esquerdo, com uma força equivalente a 75cN. A análise microscópica qualitativa avaliou os fenômenos teciduais e celulares decorrentes da movimentação dentária induzida, nos diferentes períodos de observação e entre os cortes microscópicos transversais e longitudinais. Resultados: dos fenômenos observados, as áreas hialinas tiveram expressão máxima no período de 5 dias e as reabsorções radiculares apresentaram-se exuberantes e bem demarcadas no período de 9 dias. Nos dois fenômenos, as raízes mais afetadas foram as distais, especialmente a raiz distovestibular. Conclusão: mediante o delineamento proposto, pode-se sugerir, para futuros trabalhos nesta linha de pesquisa, períodos de análise de 5 a 9 dias e cortes microscópicos transversais.Palavras-chave: Movimentação dentária induzida. Reabsorção óssea. Reabsorção radicular.

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“…We previously reported that receptor activator of nuclear factor -B ligand (RANKL) is detected in resorbed roots and PDL tissues exposed to an excessive orthodontic force(12). Hatai et al (13)suggested that apoptosis occurs on the alveolar bone resorption compression side during OTM. Therefore, apoptosis and RANKL expression may affect the incidence of ORR during OTM.…”

Section: Introductionmentioning

confidence: 99%

<b>Caspase-mediated Apoptosis by Compressive Force Induces RANKL in Cementoblasts </b>

et al. 2018

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Orthodontic root resorption (ORR) is one of the serious adverse events related to orthodontic treatment. Caspases are important effector molecules that mediate the process of apoptosis. However, the relationship between the mechanism underlying ORR and apoptosis in the cementum has not been clarified. In this study, human cementoblast-like cells (HCEMs) were cultured and subjected to pressure conditions assumed during orthodontic tooth movement in vitro. We then examined the effect of compressive force on caspase 3, caspase 8, receptor activator of nuclear factor -B ligand (RANKL) and osteoprotegerin(OPG)release. Furthermore, the association between root resorption and caspase was examined using z-VAD-fmk, a caspase inhibitor. In addition, we used HCEMs to examine the expression of caspase 3, caspase 8, RANKL and OPG under heavy force (HF)or optimum force(OF)in vitro. We then assessed the effects of HF with caspase inhibition using z-VAD-fmk [heavy force+inhibition(HI)group] on RANKL release from HCEMs. The application of HF induced higher levels of caspase 3 and 8 than OF. RANKL expression in cementoblasts was observed after the release of caspase 3 and 8 in the HF group. RANKL expression was significantly decreased protein production was suppressed in the HI group compared with the HF group (p < 0.05). The RANKL/OPG ratio was significantly decreased to about 57% at 9 h and 40% at 12 h in the HI group compared with the HF group(p < 0.05). These findings suggest that cementoblasts produce caspase 3 and 8 under HF conditions to activate the apoptotic pathway and then induce osteoclasts via RANKL. We considered that it as part of the mechanism involved in ORR.

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Apoptosis of periodontal ligament cells induced by mechanical stress during tooth movement

Cited by 38 publications

References 18 publications

Effect of Compression Force on Apoptosis in Human Periodontal Ligament Cells

Effect of Compression Force on Apoptosis in Human Periodontal Ligament Cells

Movimentação dentária experimental em murinos: período de observação e plano dos cortes microscópicos

<b>Caspase-mediated Apoptosis by Compressive Force Induces RANKL in Cementoblasts </b>

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