Apoptosis of monocytes cultured from long-term hemodialysis patients

Heidenreich, Stefan; Schmidt, Michael; Bachmann, Jürgen; Harrach, B.

doi:10.1038/ki.1996.110

Cited by 69 publications

(68 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This finding may be explained by the fact that during the hemodialysis procedure, blood exposed to less biocompatible dialysis membrane may result in activation induced cell apoptosis involving not only mononuclear and polymorphonuclear cells, but also lymphocytes [6, 7,20,21,22,23]. Memoli et al [24] have reported that in uremic patients dialyzed with bioincompatible membranes the production of IL-12 by lymphomononuclear cells is increased which reflects cell activation.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Lymphocyte Apoptosis: Role of Uremia and Permeability of Dialysis Membrane

et al. 2005

View full text Add to dashboard Cite

Background: Uremia is associated to host defense mechanism disorders. Lymphocyte apoptosis, which may cause alteration of the immune system, is increased in uremic patients. The aim of the present study was to determine if, in addition to uremia, dialysis membranes with different biocompatibility and permeability have an effect on lymphocyte apoptosis. Methods: Cell apoptosis and Fas expression were assessed using flow cytometry in four groups of patients: (1) uremic non-dialyzed (Non-D) patients; (2) hemodialysis (HD) patients on hemophan; (3) low-flux polysulfone, and (4) high-flux polysulfone membrane. Ten healthy volunteers were used ascontrols. Results: At baseline, lymphocytes from patients on hemophan showed an increase in apoptosis (18.4 ± 6.9%) as compared with Non-D (7.2 ± 2.8%; p < 0.001), low-flux (6.4 ± 2.4%; p < 0.001), high-flux (2.6 ± 1.2%; p < 0.001) and controls (2.0 ± 1.0%; p < 0.001). Fas expression was similar in lymphocytes from Non-D and hemophan dialyzed patients (40.5 ± 5% vs. 40.4 ± 6%), and in both groups it was greater than low-flux (30%±7%; p < 0.001), high-flux (11 ± 4%; p < 0.001) and controls (12.6 ± 3%; p < 0.001). When lymphocytes were cultured for 48 h, apoptosis was similar in Non-D and hemophan (27.0 ± 4.3% vs. 27.1 ± 6.9%); apoptosis of lymphocyte from patients on low-flux (14.1 ± 3.5%) was greater than on high-flux polysulfone membrane (7.0 ± 2.0%; p < 0.001). Conclusion: These findings suggest that in dialysis patients lymphocyte apoptosis is influenced not only by the biocompatibility but also by the permeability of the dialysis membrane.

show abstract

Section: Discussionmentioning

confidence: 99%

“…Different authors have reported an increase in apoptosis of mononuclear cells, neutrophils and lymphocytes [5,6,7,8,9] from uremic patients on regular hemodialysis.…”

Section: Introductionmentioning

confidence: 99%

Lymphocyte Apoptosis: Role of Uremia and Permeability of Dialysis Membrane

et al. 2005

View full text Add to dashboard Cite

show abstract

“…Instead a large number of studies using experimental models and human subjects have shown that uremia is associated with apoptosis in a wide range of cells and tissues such as skeletal muscle [19,20] , myocardium [21] , platelets [22,23] , monocytes [24] , neutro phils [25] , lymphocytes [26] , leukocytes [27] and vascular endothelial cells [28] . The kidney has also been shown as a target for apoptosis in uremia with both podocytes [29] and proximal tubular cells identified as having increased apoptotic cell death [30] .…”

Section: Uremia Induced Apoptosismentioning

confidence: 99%

Aging and uremia: Is there cellular and molecular crossover?

White

Yaqoob

Harwood

2015

WJN

View full text Add to dashboard Cite

immune systems. However, whilst much has been documented about the shared physical characteristics of aging and uremia, the molecular and cellular similarities between the two have received less attention. In order to bridge this perceived gap we have reviewed published research concerning the common molecular processes seen in aging subjects and CKD patients, with specific attention to altered proteostasis, mitochondrial dysfunction, post-translational protein modification, and senescence and telomere attrition. We have also sought to illustrate how the cell death and survival pathways apoptosis, necroptosis and autophagy are closely interrelated, and how an understanding of these overlapping pathways is helpful in order to appreciate the shared molecular basis behind the pathophysiology of aging and uremia. This analysis revealed many common molecular characteristics and showed similar patterns of cellular dysfunction. We conclude that the accelerated aging seen in patients with CKD is underpinned at the molecular level, and that a greater understanding of these molecular processes might eventually lead to new much needed therapeutic strategies of benefit to patients with renal disease. AbstractMany observers have noted that the morphological changes that occur in chronic kidney disease (CKD) patients resemble those seen in the geriatric population, with strikingly similar morbidity and mortality profiles and rates of frailty in the two groups, and shared characteristics at a pathophysiological level especially in respect to the changes seen in their vascular and REVIEW

show abstract

“…In previous reports [8, 10], we have shown that culture with CU membrane induces apoptosis in human monocytes. After 48 h of culture with the CU membrane, 47 ± 9% of the THP-1 cells undergo apoptosis (fig.…”

Section: Resultsmentioning

confidence: 99%

“…During hemodialysis with bioincompatible membranes such as Cuprophan (CU), activation of mononuclear cells results in cytokine release [1, 2, 3, 4], expression of adhesion molecules [5, 6, 7, 8], and formation of cell aggregates, and some cells undergo apoptosis [9, 10]. The mechanism(s) by which hemodialysis membranes induce mononuclear cell activation are not completely understood.…”

Section: Introductionmentioning

confidence: 99%

Role of Adhesion Molecules in Mononuclear Cell Apoptosis Induced by Cuprophan Hemodialysis Membranes

Carracedo

Ramı́rez²,

Martín‐Malo³

et al. 2001

Nephron

View full text Add to dashboard Cite

Background/Aim: Hemodialysis with Cuprophan (CU) membranes induces mononuclear cell activation, leading to increased expression of adhesion molecules, formation of cell aggregates, and apoptosis. It is likely that structure(s) of the CU membrane interact with mononuclear cell surface molecules which transduce biochemical signals to the cell. Interactions between adhesion molecules and extracellular matrix have been implicated in cell activation, proliferation, and/or apoptosis. In the present work, we study whether adhesion molecules may be involved in CU-induced mononuclear cell aggregation and/or apoptosis. Methods: The present study was performed using THP-1 cells, a human monocytic cell line, cultured in the presence of the CU membrane. CD11b and CD54 expression was studied with fluorescent monoclonal antibodies. Cell aggregation was quantified using a phase-contrast microscope. Apoptosis was evaluated by either light microscopy or annexin V labeling. Results: The results show that incubation of CU membranes with the proteins CD11b, CD18, and CD54 or the blockade of these cell surface molecules with specific monoclonal antibodies inhibited the CU-induced aggregation and apoptosis in a dose-dependent manner. Conclusion: These results suggest that CU membranes interact selectively with these specific proteins to induce cell activation which ultimately results in apoptosis.

show abstract

Apoptosis of monocytes cultured from long-term hemodialysis patients

Cited by 69 publications

References 36 publications

Lymphocyte Apoptosis: Role of Uremia and Permeability of Dialysis Membrane

Lymphocyte Apoptosis: Role of Uremia and Permeability of Dialysis Membrane

Aging and uremia: Is there cellular and molecular crossover?

Role of Adhesion Molecules in Mononuclear Cell Apoptosis Induced by Cuprophan Hemodialysis Membranes

Contact Info

Product

Resources

About