Apoptosis of leukemia K562 and Molt-4 cells induced by emamectin benzoate involving mitochondrial membrane potential loss and intracellular Ca 2+ modulation

Yun, Xinming; Rao, Wenbing; Xiao, Ciying; Huang, Qingchun

doi:10.1016/j.etap.2017.04.013

Cited by 21 publications

(7 citation statements)

References 31 publications

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“…Therefore, we measured changes in ψm in CA-treated A. flavus cells by using a JC-1 probe. As shown in Figure 4C, ψm significantly decreased after incubation with different concentrations of CA (0, 0.033, 0.065, 0.13, 0.26, and 0.52 mg/ml), and this result is consistent with the results of previous research (Yun et al, 2017). We speculated that the mitochondrial homeostasis is disrupted, causing its dysfunction and leading to changes in membrane potential.…”

Section: Discussionsupporting

confidence: 91%

Cinnamaldehyde, a Promising Natural Preservative Against Aspergillus flavus

Yang

Chen

et al. 2019

Front. Microbiol.

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The problem of food spoilage due to Aspergillus flavus (A. flavus) needs to be resolved. In this study, we found that the minimum inhibitory concentration of cinnamaldehyde (CA) that inhibited A. flavus was 0.065 mg/ml and that corn can be prevented from spoiling at a concentration of 0.13 mg/cm 3. In addition to inhibiting spore germination, mycelial growth, and biomass production, CA can also reduce ergosterol synthesis and can cause cytomembrane damage. Our intention was to elucidate the antifungal mechanism of CA. Flow cytometry, fluorescence microscopy, and western blot were used to reveal that different concentrations of CA can cause a series of apoptotic events in A. flavus, including elevated Ca 2+ and reactive oxygen species, decrease in mitochondrial membrane potential (ψ m), the release of cytochrome c, the activation of metacaspase, phosphatidylserine (PS) externalization, and DNA damage. Moreover, CA significantly increased the expression levels of apoptosis-related genes (Mst3, Stm1, AMID, Yca1, DAP3, and HtrA2). In summary, our results indicate that CA is a promising antifungal agent for use in food preservation.

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Section: Discussionsupporting

confidence: 91%

Cinnamaldehyde, a Promising Natural Preservative Against Aspergillus flavus

Yang

Chen

et al. 2019

Front. Microbiol.

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“…These results agreed with Zhang et al (2017) and Yun et al (2017), who detected apoptosis in cells of EMB human liver exposure by enhanced caspase-3 activities and increased DNA fragmentation. This agreed with Azoz et al (2020), who found that EMB oral intake by rats caused a markedly increase in DNA damage.…”

Section: Comet Assaysupporting

confidence: 91%

“…Figure 5. Relative expression of Mgst1, CYP2E1, Caspase 3 and IL1b (compar to β-actin as internal control) genes in the liver of mice treated with EMB alone or combined with natural compounds.Comet assayEmamectin administration to mice induced a marked increase in liver DNA fragmentation (Figure6and Table6).These results agreed withZhang et al (2017) andYun et al (2017), who detected apoptosis in cells of EMB human liver exposure by enhanced caspase-3 activities and increased DNA fragmentation. This agreed withAzoz et al (2020), who found that EMB oral intake by rats caused a markedly increase in DNA damage.…”

supporting

confidence: 86%

Antioxidant Activity of Some Natural Compounds in Alleviating the Hepatotoxicity Effects Induced by Emamectin Benzoate in Male Mice

M.M.Al-Akra

Zedan

2021

Journal of Agricultural Chemistry and Biotechnology

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Emamectin benzoate (EMB) is a biopesticide that is used in agriculture as an insecticide. Due to its wide range of use, it is easier to reach ecologically and affects human health. This study aims to evaluate the protective effect of natural compounds against EMB-induced hepatotoxicity. It is the first study quantifying the hepatoprotective effect of these extracts against EMB effects. Biochemical analysis includes MDA, CAT, SOD, ALT and AST analysis. However, genetic studies include the gene expression of Mgst1, CYP2E1, caspase3, IL-1β and DNA fragmentation, as well as, histopathological investigations were performed. Male mice were distributed into five groups: G1: the negative control, G2: EMB group (5mg/kg diet), G3: EMB+Boswellia serrata (90 mg/kg diet), G4: EMB+Cinnamomum zeylanicum (600mg/kg diet), G5: EMB+ powder of the snail (600mg/kg diet), and the experiment continues for eight weeks. The results appeared that EMB induced oxidative stress in the liver by increase the activity of MDA. The biomarker of liver injury ALT and AST were elevated with antioxidant enzymes inhibition. EMB produced several histopathological changes in the liver. Relative expressions of Mgst1, CYP2E, caspase-3 and IL-1β genes elevated in the liver. The increase in DNA damage was noticed as recorded by an increase in tail length, tail DNA% and tail moment. Co-treatment with natural compounds reduced the toxicity of EMB. They reduce the abnormal biochemical, histopathological, gene expression and DNA damage by increasing antioxidant capacity. Therefore, the natural compounds used in this study act as potent hepatoprotective agents against EMB induced hepatotoxicity in mice.

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“…The present study demonstrated that Ac significantly suppressed proliferation and colony formation in cRc cells. decreased mitochondrial membrane potential is a common phenomenon of mitochondrial apoptosis in cancer cells (4), which results in the release of mitochondrial apoptogens to initiate the caspase cascade, causing programmed cell death (26,27). The present study identified that AC treatment for 24 h decreased the mitochondrial membrane potential in HcT116 cells and induced cRc cell apoptosis.…”

Section: Discussionmentioning

confidence: 53%

Asiaticoside suppresses cell proliferation by inhibiting the NF‑κB signaling pathway in colorectal cancer

Zhou

et al. 2020

Int J Mol Med

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colorectal cancer (cRc) is one of the leading causes of cancer-associated mortality. Asiaticoside (Ac) exhibits antitumor effects; however, to the best of our knowledge, the biological function of Ac in cRc cells remains unclear. Therefore, the aim of the present study was to investigate the effect of Ac on cRc cells. In the present study, ccK-8 and colony formation assays were performed to assess the effects of AV on human cRc cell lines (HcT116, SW480 and LoVo). Mitochondrial membrane potential was examined by Jc-1 staining. cell apoptosis and cell cycle were monitored by flow cytometry, and the expression of genes was evaluated using RT-qPcR and western blot analysis. Furthermore, the biological effect of Ac in vivo was detected using a xenograft mouse model. The findings revealed that 2 µM AC suppressed the proliferation of cRc cells in a time-and dose-dependent manner, but had no adverse effects on normal human intestinal FHc cells at a range of concentrations. Ac decreased the mitochondrial membrane potential and increased the apoptosis of cRc cells in a dose-dependent manner. Furthermore, Ac induced cell cycle arrest at the G0/G1 phase. Ac attenuated IκBα phosphorylation in a dose-dependent manner, thereby preventing P65 from entering the nucleus, and resulting in inhibition of the NF-κB signaling pathway. In addition, Ac significantly reduced the expression of CDK4 and Cyclin D1 in a dose-dependent manner, significantly upregulated the activation of caspase-9 and caspase-3, and decreased the Bcl-2/Bax mRNA ratio. Furthermore, treatment with the NF-κB signaling pathway inhibitor JSH-23 significantly increased the cytotoxicity of Ac in cRc cells. Findings of the xenograft mice model experiments revealed that AC significantly inhibited colorectal tumor growth in a dose-dependent manner. Overall, Ac suppressed activation of the NF-κB signaling pathway by downregulating IκBα phosphorylation. This resulted in inhibition of cRc cell viability and an increase of cell apoptosis, which may form the basis of Ac use in the treatment of patients with cRc.

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Apoptosis of leukemia K562 and Molt-4 cells induced by emamectin benzoate involving mitochondrial membrane potential loss and intracellular Ca 2+ modulation

Cited by 21 publications

References 31 publications

Cinnamaldehyde, a Promising Natural Preservative Against Aspergillus flavus

Cinnamaldehyde, a Promising Natural Preservative Against Aspergillus flavus

Antioxidant Activity of Some Natural Compounds in Alleviating the Hepatotoxicity Effects Induced by Emamectin Benzoate in Male Mice

Asiaticoside suppresses cell proliferation by inhibiting the NF‑κB signaling pathway in colorectal cancer

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