Apoptosis-Inducing Effects of Two Anthraquinones from Hedyotis diffusa WILLD.

Shi, Ying; Wang, Chen-Hui; Gong, Xingguo

doi:10.1248/bpb.31.1075

Cited by 54 publications

(34 citation statements)

References 12 publications

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“…[5] HepG2 cells 2-Hydroxy-3-methyl anthraquinone 1-Methoxy-2-hydroxy anthraquinone Both compounds showed inhibitory activity against protein tyrosine kinases v-src and pp60src and arrested the growth of HepG2 cancer cells. [38] Bcap37 cells 2-Hydroxy-3-methyl anthraquinone, 1-Methoxy-2-hydroxy anthraquinone Both compounds showed inhibitory activity against protein tyrosine kinases v-src and pp60src and arrested the growth of Bcap37 cells. [38] Cervical tumor Nude mouse model Aqueous extract…”

Section: Ht-29 Cells Ethanol Extractmentioning

confidence: 99%

“…[38] Bcap37 cells 2-Hydroxy-3-methyl anthraquinone, 1-Methoxy-2-hydroxy anthraquinone Both compounds showed inhibitory activity against protein tyrosine kinases v-src and pp60src and arrested the growth of Bcap37 cells. [38] Cervical tumor Nude mouse model Aqueous extract…”

Section: Ht-29 Cells Ethanol Extractmentioning

confidence: 99%

“…As a result, ursolic acid exhibited a strong inhibition of HepG2 cell survival (IC 50 36.63 µM) [5]. Another study revealed that the inhibitory activity of 2-hydroxy-3-methyl anthraquinone (IC 50 51 µM) and 2-hydroxy-1-methoxy anthraquinone (IC 50 62 µM) might be achieved by activity against protein tyrosine kinases v-src and pp60src [38].…”

Section: Anti-liver Cancer Activitymentioning

confidence: 99%

See 2 more Smart Citations

The Hedyotis diffusa Willd. (Rubiaceae): A Review on Phytochemistry, Pharmacology, Quality Control and Pharmacokinetics

et al. 2016

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Hedyotis diffusa Willd (H. diffusa) is a well-known Chinese medicine with a variety of activities, especially its anti-cancer effect in the clinic. Up to now, 171 compounds have been reported from H. diffusa, including 32 iridoids, 26 flavonoids, 24 anthraquinones, 26 phenolics and their derivatives, 50 volatile oils and 13 miscellaneous compounds. In vitro and in vivo studies show these phytochemicals and plant extracts to exhibit a range of pharmacological activities of anti-cancer, antioxidant, anti-inflammatory, anti-fibroblast, immunomodulatory and neuroprotective effects. Although a series of methods have been established for the quality control of H. diffusa, a feasible and reliable approach is still needed in consideration of its botanical origin, collecting time and bioactive effects. Meanwhile, more pharmacokinetics researches are needed to illustrate the characteristics of H. diffusa in vivo. The present review aims to provide up-to-date and comprehensive information on the phytochemistry, pharmacology, quality control and pharmacokinetic characteristics of H. diffusa for its clinical use and further development.

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Section: Ht-29 Cells Ethanol Extractmentioning

confidence: 99%

Section: Ht-29 Cells Ethanol Extractmentioning

confidence: 99%

Section: Anti-liver Cancer Activitymentioning

confidence: 99%

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The Hedyotis diffusa Willd. (Rubiaceae): A Review on Phytochemistry, Pharmacology, Quality Control and Pharmacokinetics

et al. 2016

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“…According to TCM, heat is considered an important causative factor in HCC, and Qingre Jiedu has been administered to clear heat and detoxify the body (6). Previous pharmacological studies have demonstrated that Qingre Jiedu herbs (including Hedyotis diffusa Willd, Prunella vulgaris, Lobelia chinensis Lour and Sophora flavescens) contain anthraquinones, polysaccharides, flavonoids, alkaloids and triterpenoids that are able to inhibit tumor cell proliferation, induce cell apoptosis and suppress angiogenesis (9)(10)(11). Matrine from Sophora flavescens is able to induce the apoptosis of HepG2 cells via the upregulation of tumor protein 53, B cell lymphoma-2 (Bcl-2)-associated X protein (Bax) and Fas, and the downregulation of Bcl-2 and c-Myc (12).…”

Section: Introductionmentioning

confidence: 99%

Fuzheng Qingjie granules potentiate the anticancer effect of cyclophosphamide by regulating cellular immune function and inducing apoptosis in Hepatoma 22 tumor-bearing mice

et al. 2017

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Abstract. Fuzheng Qingjie (FZQJ) is a polyherbal Chinese medicine that has previously been implemented as an adjuvant therapy for gastrointestinal cancer. The present study investigated whether FZQJ is able to potentiate the anticancer effect of cyclophosphamide (CTX). Hepatoma 22 tumor-bearing mice were randomly divided into a vehicle group, CTX group, FZQJ group and combination (CTX+FZQJ) group. In addition, untreated mice without H22 cells served as blank controls. Seven days post-treatment, the mice were sacrificed and the tumors were weighed. Blood cells were evaluated using an automatic hemocytometer analyzer and flow cytometer. The expression levels of interleukin (IL)-2 and tumor necrosis factor (TNF)-α were evaluated using a radioimmunoassay. Apoptotic cells were observed using a terminal deoxynucleotidyl transferase dUTP nick-end labeling assay. Alanine transaminase, aspartate aminotransferase, blood urea nitrogen and creatinine were examined using an automatic biochemical analyzer. The results demonstrated that the tumor inhibitory rate and apoptosis index were higher in the combination group, compared with those in the CTX group. Notably, FZQJ was able to alleviate CTX-induced decreases in the numbers of white blood cells and platelets, CD3+ and CD4 + T lymphocyte subsets, and the concentration of hemoglobin, body weight and thymus index, and increase serum TNF-α and IL-2 levels without overt hepatorenal toxicity. These results suggest that FZQJ granules may enhance the anticancer effect of CTX, in addition to alleviating the side effects.

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“…Lin et al (2011b) indicated that Hedyotis diffusa had an antiproliferative-activity on Murine leukemia WEHI-3 cells, and could promote T-and B-cell proliferation in leukemic mice. In addition, there were several further reports about the effect of Hedyotidis diffusae herba on different cancer cells (Chen et al, 2012;Gupta et al, 2004;Lee et al, 2011;Shi et al, 2008;Wang et al, 2011;Willimott et al, 2007). Due to the broad anticancer activity of Hedyotidis diffusae herba, its chemical components have drawn our attention.…”

Section: Introductionmentioning

confidence: 99%

Anticancer effect of 2,7-dihydroxy-3-methylanthraquinone on human gastric cancer SGC-7901 cellsin vitroandin vivo

Zhu

Zheng

Zhang

et al. 2015

Pharmaceutical Biology

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Objective: The objective of this study is to study the anticancer effect and mechanism of DDMN on SGC-7901 cells. Materials and methods: The MTT assay was used to determine the effect of DDMN on cell viability of SGC-7901 cells, and the cytotoxic effect was evaluated by the IC 50 value. After treatment with different doses of DDMN (10, 20, and 40 mM) for 48 h, flow cytometry was used to investigate the apoptosis of SGC-7901 cells induced by DDMN. Further, western blotting was performed to study anticancer mechanism by assaying apoptosis-related proteins containing Mcl-1, Bcl-xl, Bcl-2, Bax, Bak, Bad, cytochrome c, caspase-3, and caspase-9. Finally, xenograft assay was used to further evaluate the effect of DDMN on SGC-7901 cells by determining body weight of nude mice, tumor volumes, and apoptosis-related proteins. Results: These results suggest that DDMN can significantly inhibit (IC 50 value ¼ 20.92 mM) the proliferation of SGC-7901 cells and induce apoptosis of SGC-7901 cells demonstrated by flow cytometry analysis. Additionally, the results of western blotting indicated that DDMN can suppress the expression of anti-apoptotic proteins Bcl-xl and Bcl-2, increase the expression of pro-apoptotic proteins Bax, Bad (40 mM), caspase-3 and caspase-9, and evidently promote the release of cytochrome c from the mitochondria to the cytoplasm. The xenograft assay further confirmed that DDMN had significant anticancer effects on SGC-7901 cells. Conclusion: DDMN had significant anticancer effect on SGC-7901 cells in vitro and in vivo related to mitochondria-mediated apoptosis.

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Apoptosis-Inducing Effects of Two Anthraquinones from Hedyotis diffusa WILLD.

Cited by 54 publications

References 12 publications

The Hedyotis diffusa Willd. (Rubiaceae): A Review on Phytochemistry, Pharmacology, Quality Control and Pharmacokinetics

The Hedyotis diffusa Willd. (Rubiaceae): A Review on Phytochemistry, Pharmacology, Quality Control and Pharmacokinetics

Fuzheng Qingjie granules potentiate the anticancer effect of cyclophosphamide by regulating cellular immune function and inducing apoptosis in Hepatoma 22 tumor-bearing mice

Anticancer effect of 2,7-dihydroxy-3-methylanthraquinone on human gastric cancer SGC-7901 cellsin vitroandin vivo

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