Apoptosis Inducers Endotoxin and Fas-Ligation Enhance the Expression of Vascular Endothelial Growth Factor in Human Endothelial Cells

Marx, Patrick; Mulder, AB; Bergh, Frank A.J.T.M. van den; Overbeeke, R.; Haanen, C.; Vermes, I.

doi:10.3109/10623329909078500

Cited by 16 publications

(12 citation statements)

References 9 publications

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“…This suggests that VEGF plays a role in the second phase of the disease, namely recovery. It has been reported that VEGF can inhibit apoptosis of endothelial cells [20] and can mediate endothelial cell proliferation, neovascularization, and can eventually induce the formation of fenestrae [18,21,22]. In addition, increased levels of VEGF might induce an increase in nitric oxide production, leading to vasodilatation and inhibition of leukocyte and platelet endothelium interactions.…”

Section: Discussionmentioning

confidence: 98%

Elevated levels of vascular endothelial growth factor in serum of patients with D+ HUS

Loo¹,

Bosma²,

Hinsbergh

et al. 2004

Pediatr Nephrol

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The pathogenesis of hemolytic uremic syndrome (D+ HUS) is characterized by endothelial damage of glomeruli and tubules within the kidney. In several other diseases in which glomerular endothelial damage occurs, elevated serum levels of vascular endothelial growth factor (VEGF) have been reported. VEGF is involved in angiogenesis, permeabilization of blood vessel endothelium, and wound repair. In this study we evaluated VEGF levels in the serum of 40 D+ HUS patients in the acute phase and during the course of the disease. VEGF levels were measured using a double-sandwich ELISA. Indirect immunohistochemistry was performed for the detection of VEGF in renal biopsy material of 3 HUS patients. Significantly elevated VEGF levels were found in HUS patients compared with controls in both serum ( P<0.001) and plasma ( P<0.05). A significant relationship was found between VEGF levels and severity of the disease according to the classification of Gianantonio ( P<0.05). Levels of VEGF in blood increased during the 2nd and 3rd week after HUS was diagnosed. Immunohistochemistry of renal biopsy material showed increased levels of the receptors for VEGF in the glomeruli. During the course of HUS, plasma VEGF levels increase and the increase is dependent on the severity of the disease. This is probably associated with the repair process.

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Section: Discussionmentioning

confidence: 98%

Elevated levels of vascular endothelial growth factor in serum of patients with D+ HUS

Loo¹,

Bosma²,

Hinsbergh

et al. 2004

Pediatr Nephrol

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“…If these alterations in endothelial cells occur in vivo as well, it seems more probable that the infected cells, instead of directly participating in formation of capillaries, might, directly or indirectly, trigger production of angiogenic factors that would induce normal, uninfected endothelial cells to form new capillaries. Addition of apoptosis‐inducing agents to endothelial cells is known to stimulate synthesis of vascular endothelial growth factor (VEGF) which, in turn, protects the cells from apoptosis (Marx et al ., 1999). Stimulation of the endothelial cells with VEGF results in tyrosine phosphorylation and recruitment of paxillin and FAK to new focal contacts (Abedi and Zachary, 1997).…”

Section: Discussionmentioning

confidence: 99%

Formation of stress fibres in human endothelial cells infected with Bartonella bacilliformis is associated with altered morphology, impaired migration and defects in cell morphogenesis

2001

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Bartonella bacilliformis, a Gram‐negative, flagellated bacterium, infects human erythrocytes (haematic phase) and endothelial cells (tissue phase), resulting in a biphasic disease. In the tissue phase of disease (verruga peruana), infection leads to infection of endothelial cells and a pronounced proliferation of these cells, resulting in characteristic skin eruptions of papules and nodules. We have studied the properties of endothelial cells infected in vitro. Extensive cytoskeletal remodelling of endothelial cells occurred after infection in vitro with B. bacilliformis. The cells became spindle shaped and contained arrays of actin stress fibres orientated parallel to the long axis of the cell. Cell–cell contacts were disrupted, along with the distribution of the plasma membrane marker protein, PECAM‐1, which participates in cell–cell junctions. The prominent stress fibres terminated in an increased number of focal contacts, which were studied using immunofluorescent staining for paxillin, a cytoplasmic protein that localizes in the focal adhesions. These morphological changes are consistent with activation of intracellular Rho by B. bacilliformis. Formation of stress fibres and the increased number of focal adhesions could be prevented by preincubation of the endothelial cells with C3 exoenzyme, which inactivates intracellular Rho by ADP ribosylation. Endothelial cell motility was greatly diminished in infected cells and the cells did not respond effectively to a stimulus that would evoke motility. In addition, infection of endothelial cells interfered with their ability to form networks of capillary tubes when suspended within three‐dimensional collagen matrices. If the properties of infected endothelial cells in vivo are similar, the infected cells will probably not participate effectively in angiogenesis.

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“…The mRNA was measured quantitatively using a competitive reverse transcription polymerase chain reaction technique as previously described 13,14 . Briefly, the cells were washed, lyzed and RNA was isolated using the QIAamp RNA Blood Kit (Qiagen, Hilden, Germany).…”

Section: Measurement Of Proliferationmentioning

confidence: 99%

Effects of fulvestrant alone or combined with different steroids in human breast cancer cellsin vitro

Jansen¹,

Franke²,

Wolbers³

et al. 2008

Climacteric

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Apoptosis Inducers Endotoxin and Fas-Ligation Enhance the Expression of Vascular Endothelial Growth Factor in Human Endothelial Cells

Cited by 16 publications

References 9 publications

Elevated levels of vascular endothelial growth factor in serum of patients with D+ HUS

Elevated levels of vascular endothelial growth factor in serum of patients with D+ HUS

Formation of stress fibres in human endothelial cells infected with Bartonella bacilliformis is associated with altered morphology, impaired migration and defects in cell morphogenesis

Effects of fulvestrant alone or combined with different steroids in human breast cancer cellsin vitro

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