Apoptosis Inducer from Streblus asper Extracts for Cancer Chemoprevention

Seeni, Azman; Zulkepli, Nur Ayunie; Wahab, Ridhwan Abdul

doi:10.1007/978-94-007-4917-7_1

Cited by 4 publications

(2 citation statements)

References 44 publications

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“…Streblus asper extract has been found to possess anti-fungal 16,17 anti-filarial 18 , anti-microbial 19 , anti-viral 20 -22 , anti-inflammatory 23 , anti-hyperglycemic 24 , and anti-diabetic properties 25 . On top of that, many previous findings have proved its ability to inhibit cancers like mouse lymphocytic leukaemia (P388 cells) 26 , osteosarcoma (HOS cells) 27 , tongue carcinoma (SCC-15 cells) 27 and human nasopharyngeal epidermoid carcinoma (KB cells) 15 .…”

Section: Introductionmentioning

confidence: 99%

Changes in the Protein Profile of Cervical Cancer Mice Xenograft Model in Response to Streblus asper Treatment

Roslan

Seeni

Ismail

et al. 2020

Journal of Natural Remedies

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Cervical cancer is the third most prevalent cancer in females (2018) with an estimation of 569,847 incidences and 311,365 deaths worldwide despite the rapid advancement of current technology in treating cervical cancer. Radiotherapy and chemotherapy pose side effects and subsequently hinder treatment efficacy. Therefore, taken together with the previous reports of the plants’ ability in treating cancers, Streblus asper is suggested to be a potential candidate for cervical cancer. This study was conducted to investigate the anti-cervical cancer potential of Streblus asper through the identification of key proteins and their expression that are regulated in the treatment using mice xenograft model. By employing the use of Liquid Chromatography Mass Spectrometry (LCMS), several proteins associated with cancer growth mechanisms were successfully identified. Four-hundred and fifty-two proteins common to both groups were identified, and 122 proteins were found able to be quantified. Among those proteins, 52 proteins were expressed more than 2-fold changes and 12 proteins were selected based on its established relationship with cancers, including annexin A2, 14-3-3 protein, transgelin-2, galectin-1, keratin, heat shock protein 10 and 70, glucose regulated protein (78kDa), gelsolin, alpha enolase, cofilin-1, vimentin, and calreticulin. All these proteins were downregulated upon treatment of cervical cancer tumour by Streblus asper. Pathway enrichment analysis revealed 40 related pathways which include among others, metabolism of protein, post-translational protein modification, cellular responses to external stimuli and stress, cell cycle, and apoptosis. These analyses may improve our molecular insight of the mechanisms involved in the treatment of cervical cancer tumour by Streblus asper extract.

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Section: Introductionmentioning

confidence: 99%

Changes in the Protein Profile of Cervical Cancer Mice Xenograft Model in Response to Streblus asper Treatment

Roslan

Seeni

Ismail

et al. 2020

Journal of Natural Remedies

Self Cite

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“…SA extract from root to leaf and its ingredients were used to cure several diseases. Research revealed that its extract is antifilarial, antifungal, antiinflammatory, antimicrobial, antiviral, antioxidant, antihyperglycemic, antidiabetic, and anticancer (Seeni et al, 2012). SA has been shown to treat A549, KB, HOS, and SCC-15 cancer cells in several investigations (Vicus et al, 2014;Yoshida et al, 2018;Priaulx et al, 2018).…”

Section: Introduction Introduction Introduction Introductionmentioning

confidence: 99%

Cytotoxic and antiproliferative effects of Streblus asper from northeastern Thailand on A549 lung cancer cells

KARIRAT,

PHANITCHA,

WIBOONCHAT

et al. 2023

Not Bot Horti Agrobo

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Thailand's second-leading cause of death is lung cancer. Thai indigenous herbal plants are sought after as an alternative treatment against lung cancer. This work aimed to examine cytotoxic, antiproliferative and antimigratory capacities of different parts of Streblus asper (SA) from northeastern Thailand on A549 lung cancer cells. Plant leaves, twigs, bark and wood were used for ethanolic extraction by maceration. The highest cytotoxicity of 85.46% was found in twig extract (IC50 57.46 µg/mL) assessed by an MTT assay. The lowest IC50 (18.42 µg/mL) was also found in twig extract using a clonogenic assay indicating its most antiproliferative activity in a long-term therapy. In addition, all SA extracts displayed antimigratory activity against A549 cells in a dose-dependent fashion, especially twig extract. Apoptotic characteristics were noticeable in SA extract treated cells. The maximum DPPH-scavenging activity, FRAP value, total phenolic and flavonoid content were found in twig extract. GC-MS analysis revealed that twig extract contained four prominent components namely ethyl-α-D-glucopyranoside, hexadecanoic acid, ethyl ester, lupeol and γ-sitosterol. Real-time PCR results showed that genes (Bcl-2, Bax, p21, and cytochrome c) linked to apoptosis were significantly affected by all SA extracts. The various SA components' ethanolic extracts exhibited moderate-to-high cytotoxic action towards A549 cells. This work will significantly advance the utilization of the plant as an alternative source of medicine for rural Thais, and it paves the way for future research to determine the active compound(s) and anticipate new drug candidates.

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Proteomic Analysis of Anti-Cancer Effects of Streblus Asper Extract on HeLa Cancer Cells

Roslan

Seeni

Ismail

et al. 2019

Biomed. Pharmacol. J.

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Cervical cancer is the third most common cancer affecting women worldwide. This occurs despite having precancerous screening and HPV vaccination implemented vigorously as a definitive intervention. Natural plant like Streblus asper has been discovered to offer great hope in treating and preventing cancers. In this study, we explored the potential of S.asper to inhibit the growth of cervical cancer cell line by using liquid chromatography mass spectrometry (LCMS). Upon analysis, seventy-six proteins that are common to both untreated and treated groups were identified. Of this, 14 proteins are found differentially expressed more than 2-fold changes. Based on past literature, we selected 7 proteins that are closely associated with treatment effects. These include Dermcidin, Keratin, type I cytoskeletal 9, Tropomyosin alpha-4 chain, Myristoylated alanine-rich C-kinase (MARCKS), Tumour protein D52, Folate receptor alpha, and Parathymosin. Pathway enrichment analysis by Reactome revealed 9 related pathways which include metabolism of protein, post-translational protein modification, signalling by Rho GTPases, signalling by NOTCH, cell cycle, cellular senescence, signalling by WNT, transcriptional regulation by TP53, and cellular responses to stress. These findings may improve our understanding on the related significant mechanism involving anti-cancer effects of S.asper on the cervical cancer cell line.

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Apoptosis Inducer from Streblus asper Extracts for Cancer Chemoprevention

Cited by 4 publications

References 44 publications

Changes in the Protein Profile of Cervical Cancer Mice Xenograft Model in Response to Streblus asper Treatment

Changes in the Protein Profile of Cervical Cancer Mice Xenograft Model in Response to Streblus asper Treatment

Cytotoxic and antiproliferative effects of Streblus asper from northeastern Thailand on A549 lung cancer cells

Proteomic Analysis of Anti-Cancer Effects of Streblus Asper Extract on HeLa Cancer Cells

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