Apoptosis induced by the monomers HEMA and TEGDMA involves formation of ROS and differential activation of the MAP-kinases p38, JNK and ERK

Samuelsen, Jan Tore; Dahl, Jon E.; Karlsson, Stig; Morisbak, Else; Becher, Rune

doi:10.1016/j.dental.2005.11.037

Cited by 123 publications

(98 citation statements)

References 35 publications

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“…The phosphorylation of p53 and increased p21 transcription are correlated to cell cycle arrest and transcriptional activation of pro-apoptotic factors. Furthermore, according to Samuelsen et al 23) exposure to HEMA (600 μM) resulted in reduced cell proliferation after 24 h and increased apoptosis after 60 h. Also, they observed phosphorylation of p53, advocating an initial DNA damage in the HEMA exposed submandibular gland cells.…”

Section: Discussionmentioning

confidence: 92%

“…In addition, Samuelsen et al 23) found increased phosphorylation of both p38 and JNK subsequent to HEMA but not to TEGDMA exposure. In both studies the monomers were tested separately.…”

Section: Discussionmentioning

confidence: 97%

“…Resin monomers have been characterized as ingredients that influence the cellular redox balance by increasing the level of ROS, while at the same time depleting the amount of the cell's major antioxidant, glutathione 6,8,23) . They also down-regulate gene expression in pulp cells and inhibit mineralization thus affecting negatively the regenerative potential of dentin/pulp complex 8,10,24) .…”

Section: Discussionmentioning

confidence: 99%

“…Spagnuolo et al 8) and Samuelsen et al 23) have reported that TEGDMA and HEMA monomers influence mitogen-activated protein (MAP)-kinase pathways. In addition, Samuelsen et al 23) found increased phosphorylation of both p38 and JNK subsequent to HEMA but not to TEGDMA exposure.…”

Section: Discussionmentioning

confidence: 99%

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Effects of a dental adhesive on cell cycle regulatory proteins

et al. 2013

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Dental bonding agents may affect the cell cycle patterns and induce cell cycle arrest by blocking its progression. This study tested the cell cycle effects through cyclin-dependent kinase (cdc2) and Rb phosphorylation. Human lung fibroblasts (MRC5) were used for the experiments. The bonding agent tested was the total-etch XP bond. Extracts of the bonding agent were prepared and serial dilutions were tested. The effects of the bonding agent on cell survival, proliferation and DNA synthesis were tested by the SRB and BrdU assays. Analysis of cell cycle distribution was performed by flow cytometry. XP bond exhibited strong inhibition of DNA synthesis and after 48 h of exposure cells were accumulated in the G2/M phase. Cells exposed to the half maximal cell growth inhibitory concentration (IC 50 ) showed an increase in cdc2 kinase and Rb phosphorylation. The results most likely indicate mutagenic effect of the tested agent.

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Section: Discussionmentioning

confidence: 92%

“…In addition, Samuelsen et al 23) found increased phosphorylation of both p38 and JNK subsequent to HEMA but not to TEGDMA exposure. In both studies the monomers were tested separately.…”

Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Effects of a dental adhesive on cell cycle regulatory proteins

et al. 2013

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“…They include tests for estimating the amount of ribonucleic acids and the damage to their chains [12], assessing the glutathione level in cells [13], assessing the expression of heat shock proteins [14], or studies evaluating the severity of apoptotic action [15]. Each of these methods has advantages and disadvantages, but provide extra information that cannot be obtained by other methods.…”

Section: Introductionmentioning

confidence: 99%

Uncompleted polymerization and cytotoxicity of dental restorative materials as potential health risk factors

Małkiewicz

Wychowański

Olkowska-Truchanowicz

et al. 2017

Ann Agric Environ Med.

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Małkiewicz K, Wychowański P, Olkowska-Truchanowicz J, Tykarska M, Czerwiński M, Wilczko M, Owoc A. Uncompleted polymerization and cytotoxicity of dental restorative materials as potential health risk factors. Ann Agric Environ Med. 2017; 24(4): 618-623. doi: 10.5604/12321966.1235159 Abstract Introduction. Composite materials used in dentistry indicate adverse biological effects in laboratory conditions. One reason for this activity is incomplete conversion of their polymer matrix, favoring chemical instability and release of biologically harmful components to the external environment. Aim. The aim of the study was to assess the degree of conversion of restorative materials commonly available on the European market and to examine the cytotoxic effects of their eluates in vitro. Material and methods. Using the Fournier transform infrared spectroscopy (FTIR) technique of analysis, the degree of polymer matrix conversion of 6 restorative materials was examined: Gradia Direct, Arkon, Filtek Z550, Herculite XRV, Tetric Evo Ceram, Charisma, polymerized with LED light. In order to assess the cytotoxicity of eluates of the studied materials obtained after 1 hour, 24 hours and 7 days, the MTT assay was used in cultured 3T3 cells. The results were statistically analyzed at significance level of p=0.05. Results. The conversion degree of the assessed polymers ranged from 31.56% for Tetric Evo Ceram to 75.84% for Arcon. The strongest (p=0.05) cytotoxic effect was demonstrated after 7-day observation of Tetric Evo Ceram eluates, reducing the metabolic activity of cells down to 56%. A positive correlation (r(x, y)=0.62) between the degree of conversion of composite materials and cytotoxic effects of their eluates on cell cultures was confirmed. Conclusions.1. Restorative dental materials are chemically unstable in the conditions of the present study. 2. Polymer-based restorative dental materials available on the European market demonstrate cytotoxic properties constituting a potential threat to the patients' health.

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Effect of recrystallization on the molecular mobility of a copolymer of vinylidene fluoride and hexafluoropropylene

Malyshkina

Бессонова

Suljanov

2010

J of Applied Polymer Sci

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Relaxation processes in copolymers of vinylidene fluoride with hexafluoropropylene (93/7) were studied by means of a dielectric method. The initial extruded film was recrystallized by free heating to temperatures above the melting point and by subsequent cooling. This increased both the perfection of the crystal phase and the degree of crystallinity. The impact of recrystallization on both the relaxation times (s's) and the activation parameters of the local mobility (b process) and microBrownian cooperative mobility in amorphous phase (a a process) was almost negligible, whereas the s's of the a c relaxation were an order of magnitude higher after recrystallization. Qualitatively, it was predicted by the soliton model for the a c relaxation. The recrystallization affected characteristics of the transition at the highest temperature (a) registered in the region of the melting of the crystals even more. The process is related to the relaxation of the space charge formed by ionogenic impurities, which migrate through the amorphous phase with high free volume. It was shown that the dynamics in the amorphous phase controlled the drift mobility of free charge carriers and, by that, determined the s of the space charge relaxation process. The structuring processes during the recrystallization also affected the parameters of the orderdisorder transition in the low-perfect ferroelectric (antiferroelectric) phase.

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Apoptosis induced by the monomers HEMA and TEGDMA involves formation of ROS and differential activation of the MAP-kinases p38, JNK and ERK

Cited by 123 publications

References 35 publications

Effects of a dental adhesive on cell cycle regulatory proteins

Effects of a dental adhesive on cell cycle regulatory proteins

Uncompleted polymerization and cytotoxicity of dental restorative materials as potential health risk factors

Effect of recrystallization on the molecular mobility of a copolymer of vinylidene fluoride and hexafluoropropylene

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