Apoptosis in Vocal Fold Polyps

Hamdan, Abdul‐Latif; Tabet, Georges; Saadeddin, Zein; Btaiche, Rachel; Khalifeh, Ibrahim

doi:10.1016/j.jvoice.2017.03.023

Cited by 4 publications

(3 citation statements)

References 20 publications

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“…[ 24 ] Changes in the microcosmic, macroscopic, and immune expression of many individuals with VFPs have been attributed to other factors such as traumatic voice behavior, smoking, and apoptosis. [ 25 ] Nonetheless, previous studies have shown that the formation of VFPs in many patients is related to GERD. [ 26 ]…”

Section: Discussionmentioning

confidence: 99%

“…Additionally, increased vascular permeability, degeneration, hypoxia, local edema, and fibrosis may contribute to the development of VFPs [24] . Changes in the microcosmic, macroscopic, and immune expression of many individuals with VFPs have been attributed to other factors such as traumatic voice behavior, smoking, and apoptosis [25] . Nonetheless, previous studies have shown that the formation of VFPs in many patients is related to GERD [26] …”

Section: Discussionmentioning

confidence: 99%

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Association between gastroesophageal reflux disease and vocal fold polyps

Zou¹,

Deng²,

Li³

et al. 2021

Medicine

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The aim of this study is to explore the relationship between gastroesophageal reflux disease (GERD) and vocal fold polyps (VFPs). This is a Case-Control study and was performed with the help of The Second Affiliated Hospital of Chongqing Medical University. Twenty-seven patients with VFP and 20 controls without VFP were recruited between May and October 2018. All the subjects underwent a saliva pepsin test, completed the GerdQ questionnaire and 24-hour multichannel intraluminal impedance with pH (24-h MII-pH) monitoring. Twenty-five resected VFP specimens were examined with immunohistochemical (IHC) and double immunofluorescence (IF) staining. The incidence of GERD in the VFP group was significantly higher than that in the control group ( P = .003). Patients with VFP had significantly higher GerdQ scores, pepsin concentrations, and pepsin-positive rates ( P < .05). Moreover, the number of proximal and upright reflux events was significantly higher in the VFP group ( P < .05). The pepsin concentration in saliva showed a significant positive correlation with the pepsin levels in tissues ( r 2 = 0.50, P = .011). Pepsin and TGF-β1-positive cells were colocalized with CD45RO-positive cells. IHC staining showed that the majority of VFP patients had a positive expression of pepsin (20/25, 80%) and pepsin-positive cells were found in both the squamous epithelium and mesenchymal tissues. IHC staining of TGF-β1 in VFP revealed findings similar to those of pepsin staining. GERD is an important risk factor for VFP. Pepsin may promote the aggregation of immune cells, increase the local cytokines, and promote inflammatory reaction, suggesting a potential new pathogenesis for VFP. The saliva pepsin test is a reliable method for GERD diagnosis.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Association between gastroesophageal reflux disease and vocal fold polyps

Zou¹,

Deng²,

Li³

et al. 2021

Medicine

View full text Add to dashboard Cite

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“…Interestingly, by evaluation of the apoptotic index in the vocal fold polyps, a higher apoptotic index was established in another study. The authors further reported simultaneous intensification of mitotic divisions, inflammation, and exocytosis when compared with the normal epithelium of the vocal folds [ 11 ]. However, the calculations of the apoptotic index and, in general, the apoptotic process in the case of vocal cord nodules, have not been established until now.…”

Section: Introductionmentioning

confidence: 99%

Singer’s Nodules: Investigating the Etiopathogenetic Markers Progressing Their Pathogenesis and Clinical Manifestations

Pilmane

Sumerags²,

Jain

et al. 2021

Biology

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Vocal nodules (or Singer’s nodules) are benign vocal cord structures which are commonly encountered by clinicians. Though phonetic trauma/abuse is thought to be the main cause of the development of vocal nodules, the exact etiopathogenesis remains unknown. Hence, we compared the immunohistochemical markers for proliferation (Ki-67), apoptosis (TUNEL), growth (EGFR), ischemia (VEGF), inflammation (IL-1α and 10), and immunoreactive innervation (PGP 9.5), in vocal nodule tissue samples obtained from 10 females (17–56 years) and vocal cord tissue from seven controls. A statistically significant increase in Ki-67, TUNEL, EGFR, VEGF and IL-1α expression was noted (p < 0.05) between nodule tissue and control tissue in both epithelial and subepithelial layers. However, the difference was non-significant for both IL-10 and PGP 9.5 (p > 0.05). All markers demonstrated moderate to strong positive correlations, except for IL-10. These findings suggest increased cellular growth and proliferation in vocal nodules coupled with a persistent presence of inflammatory and ischemic environment. Furthermore, global prevalence of apoptotic cells and decreased anti-inflammatory cytokines highlight the presence of underlying complex mechanisms in the etiopathogenesis of vocal nodules, with age having a negligible impact on the marker levels. Our results could potentially further our knowledge in understanding the effects of different treatment modalities available at the cellular level.

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