Apoptosis in the late replication phase of Bovine alphaherpesvirus 1 in experimentally infected calves

Ferreira, Hanna Carolina Campos; Araujo, Elaine Nery; Rosado, Nívia Carolina Lopes; Fietto, Juliana Lopes Rangel; Santos, Marcelo Rocha dos; Gomes, Lidiany Lopes; Silva, Laura Morais Nascimento; Bressan, Gustavo Costa; Martins, Gustavo Ferreira; Sreevatsan, Srinand; Silva-Júnior, Abelardo

doi:10.1007/s42770-021-00546-8

Cited by 2 publications

(1 citation statement)

References 22 publications

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“…As a nuclear replicating virus, BoHV-1 infection induces oxidative DNA damage in multiple cell cultures, such as bovine kidney cells (MDBK) and human A549 lung carcinoma epithelial cells, as demonstrated by the increased production of DSBs analyzed by comet assay [ 10 , 13 , 14 ]. Accumulating studies have indicated that damaged DNA can activate various cell death pathways [ 13 ], and BoHV-1infection induces diverse forms of cell death both in vivo and in vitro [ 14 , 15 , 16 , 17 , 18 , 19 ], which underscores the implication of virus-infection-induced DNA damage in the virus pathogenesis. In addition, as an oncolytic virus, BoHV-1 infection in human lung adenocarcinoma cells A549 results in cell lesions partially by inducing DNA damage [ 10 ], which underlies the importance of DNA damage in the virus oncolytic effects.…”

Section: Introductionmentioning

confidence: 99%

DNA Damage Response Differentially Affects BoHV-1 Gene Transcription in Cell Type-Dependent Manners

Tang

Yuan

et al. 2022

Biomedicines

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Bovine herpesvirus 1 (BoHV-1), an important pathogen of cattle, is also a promising oncolytic virus. Recent studies have demonstrated that the virus infection induces DNA damage and DNA damage response (DDR), potentially accounting for virus infection-induced cell death and oncolytic effects. However, whether the global DDR network affects BoHV-1 productive infection remains to be elucidated. In this study, we show that global DDR induced by ultraviolet (UV) irradiation prior to BoHV-1 infection differentially affected transcription of immediate early (IE) genes, such as infected cell protein 0 (bICP0) and bICP22, in a cell-type-dependent manner. In addition, UV-induced DDR may affect the stabilization of viral protein levels, such as glycoprotein C (gC) and gD, because the variation in mRNA levels of gC and gD as a consequence of UV treatment were not in line with the variation in individual protein levels. The virus productive infection also affects UV-primed DDR signaling, as demonstrated by the alteration of phosphorylated histone H2AX (γH2AX) protein levels and γH2AX formation following virus infection. Taken together, for the first time, we evidenced the interplay between UV-primed global DDR and BoHV-1 productive infection. UV-primed global DDR differentially modulates the transcription of virus genes and stabilization of virus protein. Vice versa, the virus infection may affect UV-primed DDR signaling.

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