Apoptosis in Porcine Pluripotent Cells: From ICM to iPSCs

Kim, Eunhye; Hyun, Sang‐Hwan

doi:10.3390/ijms17091533

Cited by 4 publications

(3 citation statements)

References 71 publications

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“…A key mediator acting in the apoptosis pathway is the tumour suppressor protein, p53. It has been found that p53‐induced intrinsic pathway occurs during the iPSCs generation and p53‐mediated regulation of the ratio of Bax versus Bcl‐2 protein level can influence the fate of a cell in response to stress (Kim & Hyun, ). Administration of Valproic acid, a HDACi, protected the cells from reprogramming‐induced senescent stress by inhibiting apoptosis through the suppression of the p16/p21 pathway (Zhai et al, ).…”

Section: Discussionmentioning

confidence: 99%

“…A key mediator acting in the apoptosis pathway is the F I G U R E 2 Determination of apoptotic and total nuclei in cloned and IVF blastocysts by TUNEL assay tumour suppressor protein, p53. It has been found that p53-induced intrinsic pathway occurs during the iPSCs generation and p53-mediated regulation of the ratio of Bax versus Bcl-2 protein level can influence the fate of a cell in response to stress (Kim & Hyun, 2016).…”

Section: Discussionmentioning

confidence: 99%

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m‐carboxycinnamic acid bishydroxamide improves developmental competence, reduces apoptosis and alters epigenetic status and gene expression pattern in cloned buffalo (Bubalus bubalis) embryos

Agrawal

Selokar

Saini

et al. 2018

Reprod Domestic Animals

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Incomplete or aberrant reprogramming of nuclear genome is one of the major problems in somatic cell nuclear transfer. In this study, we studied the effect of histone deacetylase inhibitor m-carboxycinnamic acid bishydroxamide (CBHA) on in vitro development of buffalo embryos produced by Hand-made cloning. Cloned embryos were treated with CBHA (0, 5, 10, 20 or 50 μM) for 10 hr from the start of reconstruction till activation. At 10 μM, but not at other concentrations examined, CBHA increased (p < .05) the blastocyst rate (63.77 ± 3.97% vs 48.63 ± 3.55%) and reduced (p < .05) the apoptotic index of the cloned blastocysts (8.91 ± 1.94 vs 4.36 ± 1.08) compared to untreated controls, to levels similar to those in IVF blastocysts (4.78 ± 0.74). CBHA treatment, at all the concentrations examined, increased (p < .05) the global level of H3K9ac in cloned blastocysts than in untreated controls to that observed in IVF blastocysts. Treatment with CBHA (10 μM) decreased (p < .05) the global level of H3K27me3 in cloned blastocysts than in untreated controls but it was still higher (p < .05) than in IVF blastocysts. CBHA (10 μM) treatment increased (p < .05) the relative expression level of pluripotency-related genes OCT-4 and NANOG, and anti-apoptotic gene BCL-XL, and decreased (p < .05) that of pro-apoptotic gene BAX than in untreated controls but did not affect the relative expression level of apoptosis-related genes p53 and CASPASE3 and epigenetics-related genes DNMT1, DNMT3a and HDAC1. These results suggest that treatment of cloned embryos with 10 μM CBHA improves the blastocyst rate, reduces the level of apoptosis and alters the epigenetic status and gene expression pattern.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

m‐carboxycinnamic acid bishydroxamide improves developmental competence, reduces apoptosis and alters epigenetic status and gene expression pattern in cloned buffalo (Bubalus bubalis) embryos

Agrawal

Selokar

Saini

et al. 2018

Reprod Domestic Animals

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“…Apoptosis plays important roles in mammalian oocyte maturation and preimplantation embryonic development [68]. In the latter context, apoptosis may contribute to embryonic loss [69] and affect some cellular responses [70] in embryos produced both in vitro and in vivo. ER stress-induced apoptosis has been reported in mammalian oocytes and IVM-derived embryos.…”

Section: Endoplasmic Reticulum Stress Induces Apoptosismentioning

confidence: 99%

Endoplasmic Reticulum (ER) Stress and Unfolded Protein Response (UPR) in Mammalian Oocyte Maturation and Preimplantation Embryo Development

Lin

Lee

Kang

et al. 2019

IJMS

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Mammalian oocytes and early embryos derived from in vitro production are highly susceptible to a variety of cellular stresses. During oocyte maturation and preimplantation embryo development, functional proteins must be folded properly in the endoplasmic reticulum (ER) to maintain oocyte and embryo development. However, some adverse factors negatively impact ER functions and protein synthesis, resulting in the activation of ER stress and unfolded protein response (UPR) signaling pathways. ER stress and UPR signaling have been identified in mammalian oocytes and embryos produced in vitro, suggesting that modulation of ER stress and UPR signaling play very important roles in oocyte maturation and the development of preimplantation embryos. In this review, we briefly describe the current state of knowledge regarding ER stress, UPR signaling pathways, and their roles and mechanisms in mammalian (excluding human) oocyte maturation and preimplantation embryo development.

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Substrates mimicking the blastocyst geometry revert pluripotent stem cell to naivety

Xu,

Wang,

Liu

et al. 2024

Nat. Mater.

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Naive pluripotent stem cells have the highest developmental potential but their in vivo existence in the blastocyst is transient. Here we report a blastocyst motif substrate for the in vitro reversion of mouse and human pluripotent stem cells to a naive state. The substrate features randomly varied microstructures, which we call motifs, mimicking the geometry of the blastocyst. Motifs representing mouse-blastocyst-scaled curvature ranging between 15 and 62 mm−1 were the most efficient in promoting reversion to naivety, as determined by time-resolved correlative analysis. In these substrates, apical constriction enhances E-cadherin/RAC1 signalling and activates the mechanosensitive nuclear transducer YAP, promoting the histone modification of pluripotency genes. This results in enhanced levels of pluripotency transcription factor NANOG, which persist even after cells are removed from the substrate. Pluripotent stem cells cultured in blastocyst motif substrates display a higher development potential in generating embryoid bodies and teratomas. These findings shed light on naivety-promoting substrate design and their large-scale implementation.

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Apoptosis in Porcine Pluripotent Cells: From ICM to iPSCs

Cited by 4 publications

References 71 publications

m‐carboxycinnamic acid bishydroxamide improves developmental competence, reduces apoptosis and alters epigenetic status and gene expression pattern in cloned buffalo (Bubalus bubalis) embryos

m‐carboxycinnamic acid bishydroxamide improves developmental competence, reduces apoptosis and alters epigenetic status and gene expression pattern in cloned buffalo (Bubalus bubalis) embryos

Endoplasmic Reticulum (ER) Stress and Unfolded Protein Response (UPR) in Mammalian Oocyte Maturation and Preimplantation Embryo Development

Substrates mimicking the blastocyst geometry revert pluripotent stem cell to naivety

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