Apoptosis in meningoencephalitis of Angiostrongylus cantonensis-infected mice

Chen, Ke Min; Lee, Hsiu Hsiung; Lai, Shih‐Chan; Hsu, Li Sung; Wang, Chau Jong; Liu, Jer Yuh

doi:10.1016/j.exppara.2008.03.013

Cited by 13 publications

(14 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Caspase-3 is an effector caspase that plays a crucial role during apoptosis, since it directly activates enzymes that are responsible for DNA fragmentation. Activated or cleaved caspase-3 has been used as a marker of apoptosis via immunohistochemistry in healthy tissues and in helminth-infected tissue sections (Resendes et al, 2004, Chen et al, 2008, Mkupasi et al, 2013. The absence of apoptotic inflammatory cells in non-infected control livers in the present study contrasts with the presence of apoptosis in healthy pig lymphoid tissues, and may be due to the small number of inflammatory cells present in the healthy sheep liver compared with those in lymphoid organs (Resendes et al, 2004).…”

Section: Discussioncontrasting

confidence: 52%

Fasciola hepatica induces eosinophil apoptosis in the migratory and biliary stages of infection in sheep

Escamilla

Bautista

Zafra

et al. 2016

Veterinary Parasitology

View full text Add to dashboard Cite

Section: Discussioncontrasting

confidence: 52%

Fasciola hepatica induces eosinophil apoptosis in the migratory and biliary stages of infection in sheep

Escamilla

Bautista

Zafra

et al. 2016

Veterinary Parasitology

View full text Add to dashboard Cite

“…The annexin V assay is based on phosphatidylserine (PS) emergence on the outer plasma membrane, an early event in apoptosis, PS binds with high affinity to the anticoagulant annexin V that is visualised by fluorescence microscopy or flow cytometry (Serradell et al, 2009). Activated or cleaved caspase-3 has been used as a marker of apoptosis by immunohistochemistry in healthy tissues (Resendes et al, 2004) and in helminth infected tissue sections (Chen et al, 2008;Escamilla et al, 2016). In the UC group, caspase-3 expression and the annexin V assay showed similar results with a low percentage (0.4-1.7%) of apoptotic peritoneal leucocytes.…”

Section: Discussionmentioning

confidence: 99%

Apoptosis of peritoneal leucocytes during early stages of Fasciola hepatica infections in sheep

Escamilla

Pérez–Caballero

Zafra

et al. 2017

Veterinary Parasitology

View full text Add to dashboard Cite

Several immunomodulatory properties have been described in Fasciola hepatica infections. Apoptosis has been shown to be an effective mechanism to avoid the immune response in helminth infections. The aim of the present work was to study apoptosis in peritoneal leucocytes of sheep experimentally infected with F. hepatica during the early stages of infection. Five groups (n=5) of sheep were used. Groups 2-5 were orally infected with 200 metacercariae (mc) and sacrificed at 1, 3, 9 and 18days post-infection (dpi), respectively. Group 1 was used as the uninfected control (UC). Apoptosis was detected using three different methods 1) immunocytochemistry (ICC) with a polyclonal antibody anti-active caspase-3; 2) an annexin V flow cytometry assay using the Annexin V-FITC/propidium iodide (PI); and 3) transmission electron microscopy (TEM). The differential leucocyte count revealed that the majority of peritoneal granulocytes were eosinophils, which increased significantly at 9 and 18 dpi with respect to the uninfected controls. The ICC study revealed that the percentage of caspase-3 apoptotic peritoneal leucocytes increased significantly from 3 dpi onwards with respect to the uninfected controls. The flow cytometry annexin V assay detected a very significant (P<0.001) increase of apoptotic peritoneal macrophages, lymphocytes and granulocytes, which remained higher than in the UC until 18 dpi. Transmission electron microscopy studies also confirmed the presence of apoptosis in peritoneal eosinophils at 18 dpi. This is the first report of apoptosis induced by F. hepatica in the peritoneal leucocytes of sheep in vivo. The results of this work suggest the importance of apoptosis induction for the survival of the juvenile parasites in the peritoneal migratory stages of infection.

show abstract

“…L3 larvae of A. cantonensis were obtained from its intermediate host, the wild giant African snail, Achatina fulica , captured in Guangzhou, China. The larvae within tissues were recovered by a previously reported method [ 62 , 63 ]. BALB/c mice (male, 6-week-old) were purchased from the animal center laboratory at Sun Yet-sen University, Guangzhou, China.…”

Section: Methodsmentioning

confidence: 99%

Preliminary molecular characterization of the human pathogen Angiostrongylus cantonensis

Cheng

Xiao

et al. 2009

BMC Molecular Biol

View full text Add to dashboard Cite

show abstract

Apoptosis in meningoencephalitis of Angiostrongylus cantonensis-infected mice

Cited by 13 publications

References 32 publications

Fasciola hepatica induces eosinophil apoptosis in the migratory and biliary stages of infection in sheep

Fasciola hepatica induces eosinophil apoptosis in the migratory and biliary stages of infection in sheep

Apoptosis of peritoneal leucocytes during early stages of Fasciola hepatica infections in sheep

Preliminary molecular characterization of the human pathogen Angiostrongylus cantonensis

Contact Info

Product

Resources

About