Apoptosis in immunocytes induced by several types of pesticides

Fukuyama, Tomoki; Tajima, Yukari; Ueda, Hideo; Hayashi, Kôichi; Shutoh, Yasufumi; Harada, Takanori; Kosaka, Toshio

doi:10.3109/15476910903321704

Cited by 30 publications

(15 citation statements)

References 57 publications

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“…Based on our previous report (Fukuyama et al, 2010), immunosuppressive environmental chemicals induce thymocyte apoptosis and reduced thymus weights. Thus, changes in thymus weights were analyzed as a general measure of in situ immunotoxicity from the test agents here as the thymus is a key lymphoid organ, and precursor T-cells migrate there to undergo maturation (Janeway et al, 2004).…”

Section: Discussionmentioning

confidence: 99%

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Effects of short-term oral combined exposure to environmental immunotoxic chemicals in mice

Nishino

Fukuyama

Kosaka

et al. 2013

Journal of Immunotoxicology

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People are constantly exposed to environmental chemicals through contact with the atmosphere or by ingestion of food. Therefore, when conducting safety assessments, the immunotoxic effects of combinations of chemicals in addition to toxicities produced by each chemical alone should be considered. The objective of the studies reported here were to demonstrate the combined effects of three well-known environmental immunotoxic chemicals -methoxychlor (MXC), an organochlorine compound; parathion (PARA), an organophosphate compound; and piperonyl butoxide (PBO), an agricultural insecticide synergist -by using a short-term oral exposure method. Seven-week-old Balb/cAnN mice received daily oral exposure to either one or two of the environmental immunotoxic chemicals for 5 consecutive days. On Day 2, all mice in each group were immunized with sheep red blood cells (SRBC), and their SRBC-specific IgM responses were analyzed by using an enzyme-linked immunosorbent assay and plaque-forming cell assay. T-and B-cell counts in the mouse spleens were also assessed via surface antigen expression. Mice that received MXC þ PARA and PBO þ MXC treatment showed marked decreases in SRBC-specific IgM production and T-and B-cell counts compared with those in mice that received vehicle control or the corresponding individual test substance. This suggests that simultaneous exposure to multiple environmental chemicals increases the immunotoxic effects of the chemicals compared to individual exposure.

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Section: Discussionmentioning

confidence: 99%

“…It is well known that exposure to environmental agents can compromise immunologic function (Fukuyama et al, 2010Nishino et al, 2013). For example, several animal studies have shown there are alterations of primary humoral responses induced by immunotoxicants like dioxins and pesticides (Flipo et al, 1992;Smialowicz et al, 1997).…”

Section: Introductionmentioning

confidence: 99%

Effects of short-term oral combined exposure to environmental immunotoxic chemicals in mice

Nishino

Fukuyama

Kosaka

et al. 2013

Journal of Immunotoxicology

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“…Our laboratory previously demonstrated that prior exposure to immunosuppressive environmental chemicals aggravates T cell-mediated allergic reactions 10 , mite-and chemical allergen-induced atopic dermatitis-like immunoreactions 11,12 . These data indicate that immunosuppression by environmental chemicals is closely related to the aggravation of allergic reactions.…”

Section: Introductionmentioning

confidence: 99%

Effects of prior oral exposure to combinations of environmental immunosuppressive agents on ovalbumin allergen-induced allergic airway inflammation in Balb/c mice

Fukuyama

Nishino

Kosaka

et al. 2014

Immunopharmacology and Immunotoxicology

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Humans are exposed daily to multiple environmental chemicals in the atmosphere, in food, and in commercial products. Therefore, hazard identification and risk management must account for exposure to chemical mixtures. The objective of the study reported here was to investigate the effects of combinations of three well-known environmental immunotoxic chemicals - methoxychlor (MXC), an organochlorine compound; parathion (PARA), an organophosphate compound; and piperonyl butoxide (PBO), an agricultural insecticide synergist - by using a mouse model of ovalbumin (OVA)-induced allergic airway inflammation. Four-week-old Balb/c mice were exposed orally to either one or two of the environmental immunotoxic chemicals for five consecutive days, prior to intraperitoneal sensitization with OVA and an inhalation challenge. We assessed IgE levels in serum, B-cell counts, and cytokine production in hilar lymph nodes, and differential cell counts and levels of related chemokines in bronchoalveolar lavage fluid (BALF). Mice treated with MXC + PARA or PBO + MXC showed marked increases in serum IgE, IgE-positive B-cells and cytokines in lymph nodes, and differential cell counts and related chemokines in BALF compared with mice that received the vehicle control or the corresponding individual test substances. These results suggest that simultaneous exposure to multiple environmental chemicals aggravates allergic airway inflammation more than exposure to individual chemicals. It is expected that the results of this study will help others in their evaluation of immunotoxic combinational effects when conducting assessments of the safety of environmental/occupational chemicals.

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“…Therefore, new short-term exposure protocols are needed to detect immunotoxicity. Previously, in the first stage of our studies, we developed a short-term method (administration via oral gavage for 3 days) for detecting thymocyte apoptosis induced by a typical immunosuppressant, methoxychlor (Fukuyama et al, 2010(Fukuyama et al, , 2011a. Indeed, methoxychlor induced prominent increases in several parameters indicative of induced thymocyte apoptosis, including Annexin V-FITC + cells, caspase (3/7, 8, and 9) activities, and DNA fragmentation.…”

Section: Research Articlementioning

confidence: 99%

Immunotoxicity in mice induced by short-term exposure to methoxychlor, parathion, or piperonyl butoxide

Fukuyama¹,

Kosaka²,

Hayashi³

et al. 2012

Journal of Immunotoxicology

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Apoptosis in immunocytes induced by several types of pesticides

Cited by 30 publications

References 57 publications

Effects of short-term oral combined exposure to environmental immunotoxic chemicals in mice

Effects of short-term oral combined exposure to environmental immunotoxic chemicals in mice

Effects of prior oral exposure to combinations of environmental immunosuppressive agents on ovalbumin allergen-induced allergic airway inflammation in Balb/c mice

Immunotoxicity in mice induced by short-term exposure to methoxychlor, parathion, or piperonyl butoxide

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