Apoptosis in human sperm: its correlation with semen quality and the presence of leukocytes

Ricci, Giuseppe; Perticarari, Sandra; Fragonas, E.; Giolo, Elena; Canova, S.; Pozzobon, Cristina; Guaschino, Secondo; Presani, Gianni

doi:10.1093/humrep/17.10.2665

Cited by 99 publications

(70 citation statements)

References 46 publications

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“…1). It is thought that abnormal and apoptotic spermatozoa are more likely to be phagocytosed by leukocytes (Tomlinson et al 1992, Ricci et al 2002; however, our fertility trial Figure 3 Percentage of PMNs bound to at least one sperm cell after spermatozoa were incubated at 37 8C for 30 min in SME, PBS, SP, BP protein (BP-P), BP non-protein (BP-NP), SME protein (SME-P), SME non-protein (SME-NP), SP protein (SP-P) or SP non-protein (SP-NP), followed by another incubation using similar conditions with a PMN suspension. Spermatozoa were obtained from three ejaculates from each of three stallions.…”

Section: Discussionmentioning

confidence: 99%

Equine seminal plasma reduces sperm binding to polymorphonuclear neutrophils (PMNs) and improves the fertility of fresh semen inseminated into inflamed uteri

Alghamdi¹,

Foster²,

Troedsson³

2004

Reproduction

125

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Seminal plasma (SP) is known to have immunosuppressive properties in several species. Equine SP has been reported to reduce or inhibit chemotaxis, phagocytosis and complement activity in vitro. The type and amount of the SP component that suppresses sperm-polymorphonuclear neutrophil (PMN) binding in vitro was determined, and the effect of such suppression on the fertility of mares inseminated in the presence of uterine inflammation, was analyzed. Sperm cells were suspended in either SP, semen extender or a mixture of both, and each was mixed with PMN-rich uterine secretions collected at 12 h after artificial insemination (AI). SP reduced binding between spermatozoa and PMNs significantly (P < 0.05). Fertile spermatozoa were suspended in SP or semen extender and used to inseminate mares 12 h after the induction of uterine inflammation. The pregnancy rate was normal (77%) when spermatozoa were suspended in SP, but was dramatically reduced to only 5% when spermatozoa were suspended in extender. The proteins from SP, blood plasma (BP) and a skim-milk-based semen extender (skim milk extender, SME) were precipitated by ammonium sulfate, resuspended in PBS and dialyzed. The effect of the precipitated proteins on sperm -PMN binding was compared with fresh, untreated SP. Both fresh SP, and isolated SP proteins reduced sperm-PMN binding (P < 0.001). Conversely, proteins isolated from either BP or SME did not reduce sperm -PMN binding. The different concentrations of SP proteins used showed a dose-dependent suppression of sperm -PMN binding. Concentrations of 1 mg/ml SP protein significantly reduced sperm -PMN binding and 6 mg/ml reduced the binding to a level similar to that observed with fresh whole SP (P < 0.001). Finally, SP protein digested with proteinase K resulted in the complete loss of SP suppressive activity confirming that the effective component is a proteinaceous substance.

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Section: Discussionmentioning

confidence: 99%

Equine seminal plasma reduces sperm binding to polymorphonuclear neutrophils (PMNs) and improves the fertility of fresh semen inseminated into inflamed uteri

Alghamdi¹,

Foster²,

Troedsson³

2004

Reproduction

125

View full text Add to dashboard Cite

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“…The labeling of DNA strand breaks (the terminal transferased UTP nick-end labeling (TUNEL) assay), Annexin V-propidium iodide assay and Sperm Chromatin Structure Assay are used for the detection and quantification of apoptosis in sperm (DNA fragmentation) (Erenpreisa et al 2002, Ricci et al 2002, Oosterhuis and Vermes 2004, Mahfouz 2009, Rylander et al 2009, Gatimel et al 2013, Gomez-Lopez et al 2013, Ruvolo et al 2013.…”

Section: Evaluation Of Sperm Morphologymentioning

confidence: 99%

Evaluation of morphological criteria of sperm quality before in vitro fertilization and intracytoplasmic sperm injection

Lasienë

Gedrimas

Vitkus

et al. 2013

Polish Journal of Veterinary Sciences

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The quality of sperm has a direct influence on the fertilization and developmental competence of embryos. In the literature we did not find defined criteria for evaluation of normal sperm parameters in various species of domestic mammals. Therefore we attempted to review evaluation of criteria of morphologically normal human sperm and their abnormalities. All sperm cells observed in the stained sample are classified as normal or abnormal. Any abnormalities in morphology of sperm have a negative effect on the outcome in in vitro fertilization and intracytoplasmic sperm injection. Abnormal sperm are categorized into subgroups according to the observed defects (concerning the head and/or midpiece and/or tail). Most morphologically abnormal sperm have multiple defects. This article can be considered as guideline for the manual of sperm quality evaluation in different species of domestic mammals.

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“…Several studies investigated the relationship between sperm morphology and apoptosis (Aziz et al, 2007;Chen et al, 2006;Gandini et al, 2000;Ricci et al, 2002;Sakkas et al, 2002;Shen et al, 2002;Siddighi et al, 2004). The design of these studies might not have facilitated the accurate distinction between moribund or necrotic sperm and motile sperm expressing apoptotic markers.…”

Section: Function and Relevance Of Caspase Activation In Spermatozoa mentioning

confidence: 99%

Impact of caspase activation in human spermatozoa

Grunewald

Sharma

Paasch

et al. 2009

Microscopy Res & Technique

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Caspases are central components in the apoptosis signaling cascade. The family of cysteine proteases transduces and enhances the apoptosis signal, and activation of effector caspases results in controlled cellular degradation. Although initially the presence of caspases in spermatozoa was controversially discussed in recent years, many studies demonstrated their activation in male germ cells. Activated apoptosis signaling results in decreased fertilizing capacity of the sperm. This review presents the current knowledge on the role of caspases in human sperm. Techniques of caspase monitoring are highlighted. With regard to the high impact of caspases on the sperm fertilizing potential, physiological and pathological settings of caspase activation and inactivation are discussed. Finally, the effects of depletion of caspase-positive sperm are shown with various standard and molecular sperm preparation methods. Microsc. Res. Tech. 00:000-000, 2009. V

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Apoptosis in human sperm: its correlation with semen quality and the presence of leukocytes

Abstract: Sperm apoptosis did not seem to be correlated with semen quality. In the absence of genito-urinary infection, one of the main functions of seminal leukocytes is probably to provide for the removal of apoptotic sperm.

Cited by 99 publications

References 46 publications

Equine seminal plasma reduces sperm binding to polymorphonuclear neutrophils (PMNs) and improves the fertility of fresh semen inseminated into inflamed uteri

Equine seminal plasma reduces sperm binding to polymorphonuclear neutrophils (PMNs) and improves the fertility of fresh semen inseminated into inflamed uteri

Evaluation of morphological criteria of sperm quality before in vitro fertilization and intracytoplasmic sperm injection

Impact of caspase activation in human spermatozoa

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