Apoptosis in human cumulus cells in relation to zona pellucida thickness variation, maturation stage, and cleavage of the corresponding oocyte after intracytoplasmic sperm injection

Høst, Erik; Gabrielsen, Anette; Lindenberg, Svend; Smidt‐Jensen, Steen

doi:10.1016/s0015-0282(01)03006-0

Cited by 102 publications

(77 citation statements)

References 23 publications

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“…In some studies a significantly lower incidence of apoptosis in follicles with an oocyte compared to empty ones was reported; the fertilization rate was higher in oocytes originating from the follicles with less apoptosis [3,23,24]. However, we did not observe a significant difference in the percentage of apoptotic granulosa cells between the follicles with a fertilized and those with a nonfertilized corresponding oocyte.…”

Section: Discussioncontrasting

confidence: 82%

Effect of apoptosis and reactive oxygen species production in human granulosa cells on oocyte fertilization and blastocyst development

Jančar

Kopitar

Ihan

et al. 2007

J Assist Reprod Genet

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Purpose: The aim was to establish the impact of human granulosa cell apoptosis and reactive oxygen species (ROS) production on fertilization competence of the oocyte, embryo developmental stage and implantation rate.Methods: Thirty women undergoing IVF-ET for tubal factor infertility were included; GnRH antagonists and gonadotrophins were used for ovarian stimulation. Granulosa cells were isolated from each aspirated follicle using gradient centrifugation. Apoptosis was studied by flow cytometry using annexin V and propidium iodide. ROS production was studied with hydroethidine staining and analyzed by flow cytometry.Results: There were no differences in characteristics of granulosa cells between the follicles with fertilized and nonfertilized oocytes. The analyzed characteristics of granulosa cells in corresponding follicles had no effect on embryo developmental stage on day 5. The percentage of ROS producing granulosa cells was lower in the follicles giving rise to blastocysts that resulted in implantation compared to those that did not (39.9% versus 69.9%, P = 0.031).Conclusions: Apoptosis and ROS production in granulosa cells have no significant impact on fertilization and do not correlate with the development of blastocysts. An increased

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Section: Discussioncontrasting

confidence: 82%

Effect of apoptosis and reactive oxygen species production in human granulosa cells on oocyte fertilization and blastocyst development

Jančar

Kopitar

Ihan

et al. 2007

J Assist Reprod Genet

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“…1). Moreover, expression of apoptosis-related genes in CCs reported herein was also associated with oocyte final maturation progression in several previous studies in many mammalian species (Host et al 2002, van Montfoort et al 2008, and it was even suggested as an indicator to predict human oocyte quality (Lee et al 2001). However, at this stage (GVBD), the CCs tissue maintains a proliferative phenotype (cell cycle and cellular growth functions) that prepares the prerequisite structure for rapid expansion and oocyte competence acquisition support.…”

Section: Cellular and Molecular Function Analysissupporting

confidence: 57%

Cumulus cell gene expression following the LH surge in bovine preovulatory follicles: potential early markers of oocyte competence

Assidi¹,

Dieleman²,

Sirard³

2010

Reproduction

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Cumulus cells (CCs) are essential for oocytes to reach full development competency and become fertilized. Many major functional properties of CCs are triggered by gonadotropins and governed by the oocyte. Consequently, cumulus may reflect oocyte quality and is often used for oocyte selection. The most visible function of CCs is their ability for rapid extracellular matrix expansion after the LH surge. Although unexplained, LH induces the final maturation and improves oocyte quality. To study the LH signaling and gene expression cascade patterns close to the germinal vesicle breakdown, bovine CCs collected at 2 h before and 6 h after the LH surge were hybridized to a custom-made microarray to better understand the LH genomic action and find differentially expressed genes associated with the LH-induced oocyte final maturation. Functional genomic analysis of the 141 overexpressed and 161 underexpressed clones was performed according to their molecular functions, gene networks, and cell compartments. Following real-time PCR validation of our gene lists, some interesting pathways associated with the LH genomic action on CCs and their possible roles in oocyte final maturation, ovulation, and fertilization are discussed. A list of early potential markers of oocyte competency in vivo and in vitro is thereafter suggested. These early biomarkers are a preamble to understand the LH molecular pathways that trigger the final oocyte competence acquisition process in bovine.

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“…Although morphological criteria are widely used in IVF clinics, several studies have supported a significant improvement of pregnancy outcome in oocytes with high and uniform ZP birefringence [29,30,32,48]. Using similar and good morphological and ZP criteria, the individual CCs were classified based on the pregnancy outcome of their oocytes.…”

Section: Discussionmentioning

confidence: 99%

Biomarkers of human oocyte developmental competence expressed in cumulus cells before ICSI: a preliminary study

Assidi

Montag

Ven

et al. 2010

J Assist Reprod Genet

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Purpose To identify reliable genomic biomarkers expressed in cumulus cells that accurately and non-invasively predict the oocyte developmental competence and reinforce the already used morphological criteria. Methods Eight consenting patients were selected for ovarian stimulation and ICSI procedures. Cumulus-oocyte complexes were transvaginally punctured and individually selected based on both good morphological criteria and high zona pellucida birefringence. Following ICSI, two 3-day embryos per patient were transferred. Pregnancy outcome was recorded and proven implantation was thereafter confirmed. Differential gene expression was assessed using two microarray platforms. Further real-time PCR validation, Ingenuity pathways analysis and intrapatient analysis were performed on 17 selected candidates. Results Seven genes were differentially (p≤0.05) associated to successful pregnancy and implantation. These biomarkers could be used to predict the oocyte developmental competence. Conclusions These genomic markers are a powerful reinforcement of morphological approaches of oocyte selection.Their large-scale validation could increase pregnancy outcome and single embryo transfer efficiency.

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Apoptosis in human cumulus cells in relation to zona pellucida thickness variation, maturation stage, and cleavage of the corresponding oocyte after intracytoplasmic sperm injection

Cited by 102 publications

References 23 publications

Effect of apoptosis and reactive oxygen species production in human granulosa cells on oocyte fertilization and blastocyst development

Effect of apoptosis and reactive oxygen species production in human granulosa cells on oocyte fertilization and blastocyst development

Cumulus cell gene expression following the LH surge in bovine preovulatory follicles: potential early markers of oocyte competence

Biomarkers of human oocyte developmental competence expressed in cumulus cells before ICSI: a preliminary study

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