Apoptosis in human cumulus cells (CCs) in relation to intracytoplasmic sperm injection (ICSI) outcome

Nasr, Mohammed; Khedr, Elsayed Galal E.; Mansour, Aziza; Sakr, Abd Eltawab I.; Rammah, Ahmed

doi:10.1016/j.ejogrb.2016.07.418

Cited by 1 publication

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Recent studies show that the expression of genes associated with the process of apoptosis in cumulus cells increases with woman's age [39,40]. Our study population included patients in the age range comparable to the (younger) group of women in the research presented by Moffatt et al [39].…”

Section: Discussionmentioning

confidence: 99%

Human Cumulus Cells in Long-Term In Vitro Culture Reflect Differential Expression Profile of Genes Responsible for Planned Cell Death and Aging—A Study of New Molecular Markers

Chermuła

Kranc

Jopek

et al. 2020

Cells

View full text Add to dashboard Cite

In the ovarian follicle, maturation of the oocyte increases in the presence of somatic cells called cumulus cells (CCs). These cells form a direct barrier between the oocyte and external environment. Thanks to bidirectional communication, they have a direct impact on the oocyte, its quality and development potential. Understanding the genetic profile of CCs appears to be important in elucidating the physiology of oocytes. Long-term in vitro culture of CCs collected from patients undergoing controlled ovarian stimulation during in vitro fertilization procedure was conducted. Using microarray expression analysis, transcript levels were assessed on day 1, 7, 15, and 30 of culture. Apoptosis and aging of CCs strictly influence oocyte quality and subsequently the outcome of assisted reproductive technologies (ART). Thus, particular attention was paid to the analysis of genes involved in programmed cell death, aging, and apoptosis. Due to the detailed level of expression analysis of each of the 133 analyzed genes, three groups were selected: first with significantly decreased expression during the culture; second with the statistically lowest increase in expression; and third with the highest significant increase in expression. COL3A1, SFRP4, CTGF, HTR2B, VCAM1, TNFRSF11B genes, belonging to the third group, were identified as potential carriers of information on oocyte quality.

show abstract

Section: Discussionmentioning

confidence: 99%