Apoptosis and Suppression of Tumor Growth by Artepillin C Extracted from Brazilian Propolis

Kimoto, Tetsuo; Arai, Shigeyuki; Kohguchi, Michihiro; Aga, Miho; Nomura, Yutaka; Micallef, Mark; Kurimoto, Masashi; Mito, Keiichiro

doi:10.1046/j.1525-1500.1998.00020.x

Cited by 179 publications

(145 citation statements)

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“…Clerodane diterpendoid shows a selective toxicity to tumor cells. A substance called Artepillin C has been isolated from propolis, and has been shown to have a cytotoxic effect on human gastric carcinoma cells, human lung cancer cells and mouse colon carcinoma cells in vitro (6,7).…”

Section: Discussionmentioning

confidence: 99%

“…300 BC (3). Recently, numerous biological properties of propolis have been reported including cytotoxic, antiherpes, antitumor, free radical scavenging, antimicrobial, and anti-HIV activities (4)(5)(6)(7)(8)(9)(10). Because of the wide range of biological activities, recently propolis has been extensively used in food and beverages to improve health and prevent diseases (11)(12)(13).…”

Section: Introductionmentioning

confidence: 99%

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Antiproliferation of human prostate cancer cells by ethanolic extracts of Brazilian propolis and its botanical origin

Kapur²,

Jm³

et al. 2007

Int J Oncol

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Abstract. Propolis is a resinous substance collected by bees (Apis mellifera) from various tree buds which they then use to coat hive parts and to seal cracks and crevices in the hive. Propolis, a known ancient folk medicine, has been extensively used in diet to improve health and to prevent disease. In the present study, we have evaluated the effects of ethanolic extracts of Brazilian propolis group l2 and bud resins of botanical origin (B. dracunculifolia), and propolis group 3 on proliferation of metastasis (DU145 and PC-3) and primary malignant tumor (RC58T/h/SA#4)-derived human prostate cancer cells. The strongest inhibition was observed in propolis group 3 (sample #3) extracts whereas moderate growth inhibition was observed in human prostate epithelial cells. In the RC58T/h/SA#4 cells, resins of botanical origin of propolis group 12 (sample #1) and propolis group 12 (sample #2) induced growth inhibition that was associated with S phase arrest whereas propolis group 3 (sample #3) induced growth inhibition that was associated with G2 arrest. The mechanisms of cell cycle effects of propolis were investigated. The resins of botanical origin of propolis group 12 and propolis group 12 showed similar inhibition of cyclin D1, CDK4 and cyclin B1 expression. Propolis group 3 showed higher induction of p21 expression but no inhibition of cyclin D1, CDK4 and cyclin B1 expression. The results obtained here demonstrate that the Brazilian propolis extracts have significant inhibitory effect on proliferation of human prostate cancer cells. Inhibition was achieved through regulation of protein expression of cyclin D1, B1 and cyclin dependent kinase (CDK) as well as p21. Our results indicate that the Brazilian propolis extracts show promise as chemotherapeutic agents as well as preventive agents against prostate cancer. IntroductionProstate cancer is the most common male cancer in USA and other Western countries, and the second leading cause of male cancer death in USA (1). Despite its pervasive impact, the etiology of prostate cancer and the factors that promote its progression are not well understood. To date, there is no secure way to tell whether prostate cancer, once found, should receive treatment. Although prostatectomy, radiation therapy and hormone therapy have been used for curing prostate cancer patients, recurrence and metastasis of prostate cancer are major problems in prostate cancer therapy. Current available treatment often has troubling side-effects such as urinary incontinence and erectile dysfunction. There is no effective treatment modality once the cancer has evolved into the hormone refractory stage. Preventive strategies are currently emerging. These is an increase in the usage of nutritional supplements such as soybeans, garlic, green tea and Vitamin D3 etc., to augment the prescribed anticancer therapies.Propolis is a resinous substance collected by Apis mellifera from various tree buds which they then use to coat hive parts and to seal cracks and crevices in the hive (2). Propolis has been used as ...

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Antiproliferation of human prostate cancer cells by ethanolic extracts of Brazilian propolis and its botanical origin

Kapur²,

Jm³

et al. 2007

Int J Oncol

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“…For this reason the com po sition of tropical propolis is quite different form propolis from temperate regions. Recently, tropi cal propolis has become a subject of increasing in terest for chemists and biologists (Banskota et Kimoto et al, 1998). In this paper we report the isolation and characterization of two new chalcones from propolis originating from El Salvador, Central Am erica, and their biological activity.…”

Section: Introductionmentioning

confidence: 88%

New Bioactive Chalcones in Propolis from El Salvador

Popova

Bankova

Spassov

et al. 2001

Zeitschrift Für Naturforschung C

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“…One finding indicated that DHCA exhibited inhibitory effects on renal carcinogenesis as a result of its oxy-radical scavenging property (Kimoto et al, 2000). Matsuno et al (1997) reported that DHCA was one of the effective anti-tumor compounds found in propolis and Kimoto et al (2001) and Kimoto et al (1998) showed that the DHCA is capable of reducing the mass of the tumor. Bohlmann and Jakupovic (1979) were the first to identify DHCA, which exists exclusively in Brazilian propolis (Park et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Supercritical CO2 extraction of raw propolis and its dry ethanolic extract

Paviani

Saito

Dariva

et al. 2012

Braz. J. Chem. Eng.

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-Three types of propolis extract were prepared and analyzed with respect to their global extraction yields and with respect to the concentration of the following markers: 3,5-diprenyl-4-hydroxycinnamic acid; 3-prenyl-4-hydroxycinnamic acid; 4-hydroxycinnamic acid and 4-methoxy-3,5,7-trihydroxyflavone. The extract EEP (ethanolic extract of propolis) was obtained by the conventional method from raw propolis using ethanol as solvent. The extracts (SFE) were obtained by supercritical solvent extraction from the raw propolis using supercritical carbon dioxide (sc-CO 2 ), with and without the addition of ethanol as a co-solvent. The fractionated supercritical extracts (FSCE) were obtained by fractionation (extract and raffinate) of the dry EEP with sc-CO 2 . EEP yields of 39.5% were obtained and maximum global extraction yields were 7.3% for SFE with no co-solvent, 51% for SFE with 15% ethanol and 18% for the FSCE extract fraction. The concentrations of the markers in the different extracts differed as a function of the operational parameters, indicating that the addition of co-solvent and the selectivity of sc-CO 2 could be manipulated so as to obtain extracts with the yields and concentrations of interest.

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Apoptosis and Suppression of Tumor Growth by Artepillin C Extracted from Brazilian Propolis

Cited by 179 publications

References 14 publications

Antiproliferation of human prostate cancer cells by ethanolic extracts of Brazilian propolis and its botanical origin

Antiproliferation of human prostate cancer cells by ethanolic extracts of Brazilian propolis and its botanical origin

New Bioactive Chalcones in Propolis from El Salvador

Supercritical CO2 extraction of raw propolis and its dry ethanolic extract

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