Apoptosis and Spermatogenesis: Evidence from an in Vivo Model of Testosterone Withdrawal in the Adult Rat

Troiano, Leonarda; Fustini, Mattia; Lovato, E.; Frasoldati, Andrea; Malorni, Walter; Capri, Miriam; Grassilli, Emanuela; Marrama, P; Franceschi, Claudio

doi:10.1006/bbrc.1994.2074

Cited by 93 publications

(39 citation statements)

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“…Thus the decrease in the cell number by apoptosis may be associated with cell proliferation. This tendency is compatible with the rat pancreas after duct ligation (WALKER and POUND, 1983) and other tissues (ABE and TAKANO, 1989;GRASSILLI et al, 1992;TROIANO et al, 1994). It may be reasonable to assume that the cell death stimulates cell proliferation for tissue regeneration.…”

Section: Discussionsupporting

confidence: 63%

Apoptosis of Mouse Pancreatic Acinar Cells after Duct Ligation.

Abe

Watanabe

1995

Archives of Histology and Cytology

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Section: Discussionsupporting

confidence: 63%

Apoptosis of Mouse Pancreatic Acinar Cells after Duct Ligation.

Abe

Watanabe

1995

Archives of Histology and Cytology

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“…The output of spermatozoa is determined largely by the shifts in degeneration vs survival of dividing and developing germ cells (Bartke 1995). The survival of spermatogenic cells is dependent on gonadotropins as well as on intratesticular androgens induced by LH (Tapanainen et al 1993, Troiano et al 1994, Billig et al 1995, Marathe et al 1995. Testosterone plays an essential role in preventing apoptotic cell death in androgen-dependent tissues (reviewed in Tenniswood et al 1992, Thompson 1994.…”

Section: Discussionmentioning

confidence: 99%

Studies on the European hare. 56. Seasonal variation of testicular activity in European brown hare Lepus europaeus

Blöttner¹,

Faber²,

Roelants³

2000

Acta Theriol.

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Testicular activity in brown hare Lepus europaeus Pallas, 1778 was studied during the annual cycle. Testicular mass, spermatozoa and testosterone were estimated. Percentages of haploid, diploid and tetraploid cells were monitored using DNA flow cytometry and the proportions of somatic and spermatogenetic cells were determined after selective labelling of somatic cells. Testis mass was high from January to July and declined thereafter to the nadir in September. Testis growth was reactivated significantly in December. Changes in testis mass corresponded with the spermatogenic efficacy (spermatozoa/g testis). High spermatogenic activity was characterized by intensive meiotic transformation of spermatocytes to spermatids, high percentages of haploid cells and low proportions of cells in the G2/M phase of mitosis. Proportions of haploid cells declined rapidly during the testis involution. Spermatogenesis was newly activated from November. The proportions as well as the ratios of spermatogenic and somatic cells differed significantly between the periods of testis involution and recrudescence. Testosterone level showed a pronounced increase in autumn preceding the intensification of spermatogenesis; the lowest concentration was found during prominent testis involution in August. The results suggest that the regulation of seasonal testicular activity is characterized by co-ordinated shifts in the relationships between mitosis, meiosis and testosterone production.

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“…An important function of testosterone is the conversion of round spermatids to elongated ones in the rat [25]. Testosterone also plays an important role in preventing apoptotic cell death in androgendependent tissues [26][27][28]. In this study, all types of spermatogenic cells were found, including mature-phase spermatozoa in seminiferous tubules of all testes, indicating that the adult GM pig has the ability to produce germ cells and is a c o n t i n u o u s b r e e d e r t h r o u g h o u t t h e y e a r .…”

Section: T H I S I S T H E F I R S T I M M U N O H I S T O C H E M I mentioning

confidence: 99%

Immunolocalization of Steroidogenic Enzymes P450scc, 3.BETA.HSD, P450c17, and P450arom in Goettingen Miniature Pig Testes

Wang

Medan

Watanabe

et al. 2005

Journal of Reproduction and Development

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Abstract. The objective of this study was to investigate immunolocalization of steroidogenic enzymes in Göttingen miniature (GM) pig testes. Testes of 6 adult GM pigs were obtained in September 1996 (n=2), February (n=2) and June (n=2), 1997. Steroidogenic enzymes were immunolocalized using polyclonal antisera raised against bovine adrenal cholesterol side-chain cleavage cytochrome P450 (P450scc), human placental 3β-hydroxysteroid dehydrogenase (3βHSD), porcine testicular 17α-hydroxylase cytochrome P450 (P450c17), and human placental aromatase cytochrome P450 (P450arom). Histologically, all types of spermatogenic cells including mature-phase spermatozoa in seminiferous tubules were observed in all testes throughout the year. Moreover, P450scc, 3βHSD, P450c17and P450arom were identified in Leydig cells but not in Sertoli cells of all testes. These results suggested that adult GM pig testes have the ability to produce germ cells throughout the year, and the synthesis of progestin, androgen and estrogen occurs in the Leydig cells of GM pig testes.

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Apoptosis and Spermatogenesis: Evidence from an in Vivo Model of Testosterone Withdrawal in the Adult Rat

Cited by 93 publications

References 0 publications

Apoptosis of Mouse Pancreatic Acinar Cells after Duct Ligation.

Apoptosis of Mouse Pancreatic Acinar Cells after Duct Ligation.

Studies on the European hare. 56. Seasonal variation of testicular activity in European brown hare Lepus europaeus

Immunolocalization of Steroidogenic Enzymes P450scc, 3.BETA.HSD, P450c17, and P450arom in Goettingen Miniature Pig Testes

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