Apoptosis and lysosome membrane permeabilization induction on breast cancer cells by an anticarcinogenic Bowman–Birk protease inhibitor from Vigna unguiculata seeds

Joanitti, Graziella Anselmo; Azevedo, Ricardo Bentes; Freitas, Sonia María de

doi:10.1016/j.canlet.2009.12.017

Cited by 57 publications

(74 citation statements)

References 20 publications

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“…In contrast, MTX-CS-NPs induced more significant signs of apoptosis, increased the reduction in cell proliferation and also arrested the cell cycle in the S-phase. Altogether, these data indicated that free MTX acted mainly as a cytostatic drug on HeLa and MCF-7 cancer cells, suppressing DNA synthesis and cellular growth, whereas the MTX loaded into CS-NPs induced both cytostatic and cytotoxic effects [45,46]. Finally, we showed that the LMP effect The assessment of the hemocompatibility of a nanocarrier is indispensable for frequent intravenous dosing.…”

Section: Discussionmentioning

confidence: 70%

In vitro antitumor activity of methotrexate via pH-sensitive chitosan nanoparticles

et al. 2013

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Nanoparticles with pH-sensitive behavior may enhance the success of chemotherapy in many cancers by efficient intracellular drug delivery. Here, we investigated the effect of a bioactive surfactant with pH-sensitive properties on the antitumor activity and intracellular behavior of methotrexate-loaded chitosan nanoparticles (MTX-CS-NPs). NPs were prepared using a modified ionotropic complexation process, in which was included the surfactant derived from N α ,N ε -dioctanoyl lysine with an inorganic lithium counterion. The pH-sensitive behavior of NPs allowed accelerated release of MTX in an acidic medium, as well as membrane-lytic pH-dependent activity, which facilitated the cytosolic delivery of endocytosed materials. Moreover, our results clearly proved that MTX-CS-NPs were more active against the tumor HeLa and MCF-7 cell lines than the free drug. The feasibilty of using NPs to target acidic tumor extracellular pH was also shown, as cytotoxicity against cancer cells was greater in a mildly acidic environment. Finally, the combined physicochemical and pH-sensitive properties of NPs generally allowed the entrapped drug to induce greater cell cycle arrest and apoptotic effects. Therefore, our overall results suggest that pH-sensitive MTX-CS-NPs could be potentially useful as a carrier system for tumor and intracellular drug delivery in cancer therapy.

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Section: Discussionmentioning

confidence: 70%

In vitro antitumor activity of methotrexate via pH-sensitive chitosan nanoparticles

et al. 2013

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“…BBI suppresses the activity of membrane-type serine protease 1, which plays a signifi cant role in cancer invasion and metastasis [Yamasaki et al, 2003], and proteasome function resulting in suppression of breast cancer cell growth [Chen et al, 2005]. In addition, BBI enhances apoptosis and lysosome membrane permeabilization in MCF-7 breast cancer cells [Joanitti et al, 2010].…”

Section: Non-nutritional Compounds Of Protein Origin Protease Inhibitorsmentioning

confidence: 99%

Non-Nutritive Compounds in Fabaceae Family Seeds and the Improvement of Their Nutritional Quality by Traditional Processing – a Review

Gulewicz¹,

Martínez‐Villaluenga²,

Kasprowicz‐Potocka³

et al. 2014

Pol. J. Food Nutr. Sci.

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“…It presents rich biotechnological potential as an anticarcinogenic agent, as a guanylin-enhancing natriuresis and renal functional response in rats, as a bradykinin-potentiating molecule, and as an insecticide, inhibiting the growth of larvae with agricultural importance [7][8][9][10][11][12].…”

Section: Introductionmentioning

confidence: 99%

Oligomerization affects the kinetics and thermodynamics of the interaction of a Bowman-Birk inhibitor with proteases

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Pires

Furtado

et al. 2017

Archives of Biochemistry and Biophysics

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., C. (2017) Oligomerization affects the kinetics and thermodynamics of the interaction of a Bowman-Birk inhibitor with proteases. Archives of Biochemistry and Biophysics, 618, pp. 9-14. (doi:10.1016/j.abb.2017.01.009) This is the author's final accepted version.There may be differences between this version and the published version. You are advised to consult the publisher's version if you wish to cite from it. to the micromolar range, secondary endothermic events become prevalent and affect both the kinetics and thermodynamics of protein associations. Our study reinforces that BBI interactions with serine proteinases should be studied in dilute solutions to abridge often neglected secondary interactions.

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Apoptosis and lysosome membrane permeabilization induction on breast cancer cells by an anticarcinogenic Bowman–Birk protease inhibitor from Vigna unguiculata seeds

Cited by 57 publications

References 20 publications

In vitro antitumor activity of methotrexate via pH-sensitive chitosan nanoparticles

In vitro antitumor activity of methotrexate via pH-sensitive chitosan nanoparticles

Non-Nutritive Compounds in Fabaceae Family Seeds and the Improvement of Their Nutritional Quality by Traditional Processing – a Review

Oligomerization affects the kinetics and thermodynamics of the interaction of a Bowman-Birk inhibitor with proteases

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