Apoptosis and Expression of BCL-2 in Human Endometrium in Natural and Artificial Cycles

Havelka, P; Oborná, I; Březinová, Jana; Lichnovský, V

doi:10.5507/bp.2005.047

Cited by 4 publications

(3 citation statements)

References 28 publications

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“…Moreover, the vesiculated cells showed loosening of their cell contacts, resulting from smaller numbers of desmosomes, less interdigitation, and marked membrane irregularity with intercellular space enlargement, also consistent with holocrine secretion. These results are in line with the increase in cell death markers and decrease in proliferation markers found by other authors during the IW (TUNEL 47 ; Bax 48 ; Fas 49 ; Ki-67, and bcl-2 50,51 ). Interestingly, our observations of holocrine secretion, may also explain the presence histones in uterine secretions.…”

Section: Discussionsupporting

confidence: 92%

Human Endometrium Ultrastructure During the Implantation Window: A New Perspective of the Epithelium Cell Types

et al. 2011

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The human endometrium is a hormonally regulated tissue that cyclically growths and differentiates in order to become a structure adequate for implantation. Molecular studies have shown some contradicting results and thus a reappraisal of the endometrium ultrastructure is warranted. In our study, endometrium biopsies were taken during the implantation window of 10 healthy women of reproductive age and analyzed using electron microscopy. Our results showed that during implantation window, the endometrial epithelium encompassed 4 cell types: microvilli-rich cells, pinopode cells, vesiculated cells, and ciliated cells. These cells showed signs of active communication with their external environment and neighboring cells, such as endocytosis, transcytosis and exocytosis. We highlighted important differences between surface and glandular epitheliums and characterized apocrine and holocrine secretion. It is likely that the features described reflect distinct functions in endometrium physiology that should be taken into account in the evaluation of the endometrium during the implantation window.

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Section: Discussionsupporting

confidence: 92%

Human Endometrium Ultrastructure During the Implantation Window: A New Perspective of the Epithelium Cell Types

et al. 2011

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“…Bcl-2 is an oncogene, and it can inhibit apoptosis [30–32]. It can reduce oxygen free radicals, block the intracellular Ca 2+ influx into cell organelles, inhibit p53-dependent apoptosis, and antagonize c-myc [33–35].…”

Section: Discussionmentioning

confidence: 99%

The expression levels of stem cell markers importin13, c-kit, CD146, and telomerase are decreased in endometrial polyps

Yuan

2011

Med Sci Monit

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SummaryBackgroundTo investigate the expression levels of importin13 (IPO13), c-kit, CD146, telomerase, caspase-3, bcl-2 and bax in endometrial polyps (EPs).Material/MethodsWe detected the mRNA expression levels of IPO13, c-kit, bcl-2 and bax in endometrial polyps (EPs) using real-time PCR. We detected the protein expression levels of IPO13, telomerase, CD146, caspase-3, bcl-2 and bax in EPs using S-P (Streptavidin-Peroxidase) immunohistochemistry. Western blotting was performed to determine the levels of importin13 and bcl-2 proteins in EPs.ResultsThe expression levels of IPO13, c-kit, telomerase, caspase3, and bax were lower in the EP tissue compared to normal endometrial tissue during the proliferation and secretion phases of the menstrual cycle (p<0.05). The expression of CD146 was decreased in the EP tissue compared to the normal endometrial tissue during the proliferation phase of the menstrual cycle (p<0.05). The expression of bcl-2 was increased in the EP tissue compared to the normal endometrial tissue during the proliferation and secretion phases of the menstrual cycle (p<0.05).ConclusionsThe expression levels of IPO13, c-kit, telomerase, caspase3, and bax were decreased; however, the expression of bcl-2 was increased in the EP tissue compared to the normal endometrial tissue. These findings suggest that the development of EPs is associated with the deregulated activities of the endometrial stem/progenitor cells and the decreased apoptosis of endometrial cells, with the latter being the major factor involved in the development of EPs.

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“…Analyzing the expression of these markers in cyclical endometrium and in hyperplasia and with carcinoma will enable in identifying the specificity and sensitivity of these markers in determining the malignant potential [18] as well as in the treatment modalities specific against these markers. The Present study attempts to determine the relationship between proliferation and the inhibition of apoptosis in normal endometrium and hyperplasia, using monoclonal antibodies against the proliferation marker, Ki-67 and the anti-apoptotic protein, Bcl-2 and to determine the expression of B-cell lymphoma 2 (Bcl -2) and Ki -67 in cyclical endometrium in proliferative and secretory phase.…”

Section: Introductionmentioning

confidence: 99%

Expression of BCL-2 and KI-67 in Cyclical Endometrium and in Endometrial Hyperplasia

Shalini

Suresh

Lilly

2021

JPRI

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The Present study attempts to determine the relationship between proliferation and the inhibition of apoptosis in normal endometrium and hyperplasia, using monoclonal antibodies against the proliferation marker, Ki-67 and the anti-apoptotic protein, Bcl-2 and to determine the expression of B-cell lymphoma 2 (Bcl – 2) and Ki – 67 in cyclical endometrium in proliferative and secretory phase. The Present study includes the expression of Bcl – 2 and Ki – 67 in endometrial hyperplasia.

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Apoptosis and Expression of BCL-2 in Human Endometrium in Natural and Artificial Cycles

Cited by 4 publications

References 28 publications

Human Endometrium Ultrastructure During the Implantation Window: A New Perspective of the Epithelium Cell Types

Human Endometrium Ultrastructure During the Implantation Window: A New Perspective of the Epithelium Cell Types

The expression levels of stem cell markers importin13, c-kit, CD146, and telomerase are decreased in endometrial polyps

Expression of BCL-2 and KI-67 in Cyclical Endometrium and in Endometrial Hyperplasia

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