Apoptosis and eryptosis: similarities and differences

Tkachenko, Anton

doi:10.1007/s10495-023-01915-4

Cited by 9 publications

(6 citation statements)

References 321 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The main hypotheses include the potential involvement of the trace element Se, iodine deficiency, mycotoxin, like T-2 toxin, contamination in grain, and fulvic acid water contamination ( 17 ). Various conditions and factors, such as DNA damage, hypoxia, loss of integrin signalling, and activation of death receptors can induce apoptosis activation ( 18 ). The analysis of articular cartilage from adult KBD patients revealed the presence of apoptotic chondrocytes in regions exhibiting proteoglycan depletion ( 19 ).…”

Section: Pathogenesis and Pathological Findings Of Kbdmentioning

confidence: 99%

Effects of selenium and iodine on Kashin-Beck disease: an updated review

Liu,

Luo,

Wen

et al. 2024

Front. Nutr.

View full text Add to dashboard Cite

Kashin-Beck disease (KBD) is an endochondral osteogenesis disorder characterised by epiphysis damage and secondary deformable arthropathy induced by multiple external factors, among which selenium (Se) and iodine deficiency are important influencing factors. Iodine deficiency is usually accompanied by a low Se content in the soil in the KBD areas of China. Se can reverse oxidative damage to chondrocytes. In addition, Se is related to the bone conversion rate and bone mineral density. Low Se will hinder growth and change bone metabolism, resulting in a decrease in the bone conversion rate and bone mineral density. Thyroid hormone imbalance caused by thyroid dysfunction caused by iodine deficiency can damage bone homeostasis. Compared with Se deficiency alone, Se combined with iodine deficiency can reduce the activity of glutathione peroxidase more effectively, which increases the vulnerability of chondrocytes and other target cells to oxidative stress, resulting in chondrocyte death. Clinical studies have shown that supplementation with Se and iodine is helpful for the prevention and treatment of KBD.

show abstract

Section: Pathogenesis and Pathological Findings Of Kbdmentioning

confidence: 99%

Effects of selenium and iodine on Kashin-Beck disease: an updated review

Liu,

Luo,

Wen

et al. 2024

Front. Nutr.

View full text Add to dashboard Cite

show abstract

“…Eryptosis characteristics include sustained membrane integrity, calpain-mediated membrane blebbing, K + efflux-dependent cell shrinkage, and the above-mentioned membrane asymmetry with PS externalization (Lang and Lang, 2015a;Scovino et al, 2022;Tkachenko, 2023;. Eryptotic signalling relies on several interconnected mediators.…”

Section: Brief Overview Of Eryptosismentioning

confidence: 99%

“…Multiple studies have suggested a critical role of Ca 2+ signalling. Within eryptosis, it has been linked to cell shrinkage via Gardos channels (Ca 2+ -sen-sitive potassium selective channels in RBCs), membrane lipid scrambling, and calpain activation (Repsold and Joubert, 2018;Föller and Lang, 2020;Dreischer et al, 2022;Tkachenko, 2023). Ca 2+ inflow can be stimulated by prostaglandin E2 or shear stress (with participation of PIEZO1 channels) (Föller and Lang, 2020).…”

Section: Brief Overview Of Eryptosismentioning

confidence: 99%

“…Therefore, the type of erythrocyte death can have a profound effect on immune response modulation. Experimental evidence suggests that erythrocytes can undergo two RCDs: apoptosis (eryptosis) (Lang and Lang, 2015b;Qadri et al, 2017;Föller and Lang, 2020;Alghareeb et al, 2023;Tkachenko, 2023; and necroptosis (LaRocca et al, 2014(LaRocca et al, , 2015(LaRocca et al, , 2016McCaig et al, 2019;Seo et al, 2023). Apoptosis is relatively immunologically inert due to its non-lytic nature and lack of DAMPs release.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Redox Status of Erythrocytes as an Important Factor in Eryptosis and Erythronecroptosis

Tkachenko,

Havránek

2023

Fol. Biol.

Self Cite

View full text Add to dashboard Cite

Overall, reactive oxygen species (ROS) signalling significantly contributes to initiation and modulation of multiple regulated cell death (RCD) pathways. Lately, more information has become available about RCD modalities of erythrocytes, including the role of ROS. ROS accumulation has therefore been increasingly recognized as a critical factor involved in eryptosis (apoptosis of erythrocytes) and erythronecroptosis (necroptosis of erythrocytes). Eryptosis is a Ca2+-dependent apoptosis-like RCD of erythrocytes that occurs in response to oxidative stress, hyperosmolarity, ATP depletion, and a wide range of xenobiotics. Moreover, eryptosis seems to be involved in the pathogenesis of multiple human diseases and pathological processes. Several studies have reported that erythrocytes can also undergo necroptosis, a lytic RIPK1/RIPK3/MLKL-mediated RCD. As an example, erythronecroptosis can occur in response to CD59-specific pore-forming toxins. We have systematically summarized available studies regarding the involvement of ROS and oxidative stress in these two distinct RCDs of erythrocytes. We have focused specifically on cellular signalling pathways involved in ROS-mediated cell death decisions in erythrocytes. Furthermore, we have summarized dysregulation of related erythrocytic antioxidant defence systems. The general concept of the ROS role in eryptotic and necroptotic cell death pathways in erythrocytes seems to be established. However, further studies are required to uncover the complex role of ROS in the crosstalk and interplay between the survival and RCDs of erythrocytes.

show abstract

“…There is no general consensus on the methodology applied to screen the cytotoxicity of nanomaterials. To assess the cytotoxicity of micro akes, we used both nucleated and enucleated cells due to the differences in their survival/cell death-regulating pathways (Tkachenko 2024a). This might reveal some cell type-speci c features of micro akes-mediated cytotoxicity.…”

Section: Introductionmentioning

confidence: 99%

Nanostructured Zinc Carbonate Hydroxide Microflakes: Assessing the Toxicity Against Erythrocytes and L929 Cells in Vitro

Prokopiuk,

Onishchenko,

Pazura

et al. 2024

Preprint

View full text Add to dashboard Cite

Introduction. Nanostructured materials have been suggested to be used as a source of dietary zinc for livestock animals. In this study, we assessed the cytotoxicity of newly synthesized nanostructured zinc carbonate hydroxide (ZnCH) Zn5(CO3)(OH)6 microflakes. Materials and methods. Cytotoxicity of the microflakes was assessed against murine L929 cell line and rat mature erythrocytes. MTT and neutral red uptake assays, scratch assay, Annexin V-FITC/7-aminoactinomycin D staining, 2',7'-dichlorodihydrofluorescein diacetate (H2DCFDA) staining, Fura 2-AM staining, staining for reactive nitrogen species (RNS), detection of caspases, and lipid order-sensitive NR12S probe staining were employed to evaluate cell viability, motility, cell death pathways, implication of Ca2+, reactive oxygen species (ROS) and RNS signaling, caspases, and alterations of cell membranes following exposure to the microflakes, respectively. To assess hemocompatibility of the Zn-containing microflakes, osmotic fragility assay and hemolysis assay were performed, as well as multiple eryptosis parameters were evaluated. Results. Our findings indicate a dose-response cytotoxicity of ZnCH microflakes against L929 cells with no toxicity observed for low concentrations (10 mg/L and below). At high concentrations, ZnCH microflakes promoted oxidative stress, Ca2+- and caspase-dependent apoptosis and altered lipid membranes. Surprisingly, toxicity to enucleated mature erythrocytes was found to be lower compared to L929 cells. ZnCH microflakes induced eryptosis associated with oxidative stress, Ca2+ signaling and recruitment of caspases. Eryptosis assays were found to be more sensitive than evaluation of hemolysis. Conclusions. Zn5(CO3)(OH)6 microflakes show no cytotoxicity at low concentrations indicating their potential as a source of zinc for livestock animals.

show abstract

Apoptosis and eryptosis: similarities and differences

Cited by 9 publications

References 321 publications

Effects of selenium and iodine on Kashin-Beck disease: an updated review

Effects of selenium and iodine on Kashin-Beck disease: an updated review

Redox Status of Erythrocytes as an Important Factor in Eryptosis and Erythronecroptosis

Nanostructured Zinc Carbonate Hydroxide Microflakes: Assessing the Toxicity Against Erythrocytes and L929 Cells in Vitro

Contact Info

Product

Resources

About