Apoptosis and DNA intercalating activities of novel emodin derivatives

Narender, Tadigoppula; Sukanya, P.; Sharma, Komal; Bathula, Surendar Reddy

doi:10.1039/c3ra23149f

Cited by 15 publications

(7 citation statements)

References 24 publications

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“…Phenolic and flavonoid compounds might be responsible for the potent antiproliferative activity of V. denticulata . The preparative HPLC of HF-2 of V. denticulata yielded a single compound, emodin (60.6 mg, R t 19.62 min) which was characterized by the 1 H and 13 C-NMR spectra comparing with the reference data [ 10 , 11 ]. The chemical structure of emodin was shown in Figure 2 .…”

Section: Resultsmentioning

confidence: 99%

Potent Antiproliferative Effect on Liver Cancer of Medicinal Plants Selected from the Thai/Lanna Medicinal Plant Recipe Database “MANOSROI III”

Manosroi

Akazawa

Kitdamrongtham

et al. 2015

Evidence-Based Complementary and Alternative Medicine

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Thai/Lanna medicinal plant recipes have been used for the treatment of several diseases including liver cancer. In this study, methanolic extracts (MEs) of 23 plants were tested for antiproliferative activity on human hepatoma cell line (Hep G2) by the sulforhodamine B (SRB) assay. Nine MEs with potent antiproliferative activity (IC50 < 100 µg/mL) were obtained and further semipurified by liquid/liquid partition extraction. The semipurified fractions were tested for the antiproliferative and antioxidative activities. ME of Stemona collinsae and the semipurified extract and methanol-water fraction (MF) of Gloriosa superba gave the highest antiproliferative activity on HepG2 which were 4.79- and 50.07-fold cisplatin, respectively. The semipurified fractions showed an increased antiproliferative activity. MF of Caesalpinia sappan and HF of Senna alata showed the highest free radical scavenging and metal chelating activities, respectively. The compound in n-hexane fraction (HF) of Ventilago denticulata which showed an increase in antiproliferative activity comparing to its ME was isolated and identified as emodin. This study has demonstrated the potential of the ME from S. collinsae, MF from G. superba, and emodin isolated from V. denticulata, for further development as an antiliver cancer agent.

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Section: Resultsmentioning

confidence: 99%

Potent Antiproliferative Effect on Liver Cancer of Medicinal Plants Selected from the Thai/Lanna Medicinal Plant Recipe Database “MANOSROI III”

Manosroi

Akazawa

Kitdamrongtham

et al. 2015

Evidence-Based Complementary and Alternative Medicine

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“…Cell viability was assessed by MTT assay [10], which is based on the reduction of MTT by mitochondrial dehydrogenases of viable cells to form a purple formazan product. Briefly MCF-7, MDA-MB-231 and HEK-293 cells (5 × 10 3 /well) were plated in 96 well plates.…”

Section: Mtt Assay For Cell Viabilitymentioning

confidence: 99%

Anticancer Effects of Extracts from the Fruit of Morinda Citrifolia (Noni) in Breast Cancer Cell Lines

et al. 2015

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Morinda citrifolia L. (NONI) fruits have been used for thousands of years for the treatment of many health problems including cancer, cold, diabetes, flu, hypertension, and pain. Plant extracts have reported several therapeutic benefits, but extraction of individual compound from the extract often exhibits limited clinical utility as the synergistic effect of various natural ingredients gets lost. They generally constitute polyphenols and flavonoids. Studies have suggested that these phytochemicals, especially polyphenols, display high antioxidant properties, which help to reduce the risk of degenerative diseases, such as cancer and cardiovascular diseases. Several in-vitro and in-vivo studies have shown that Noni fruits have antioxidant, anti-inflammatory, anti-dementia, liver-protective, anticancer, analgesic, and immunomodulatory effects. Till date about 7 in vitro cancer studies have been done, but a detailed in vitro study including cell cycle and caspase activation assay on breast cancer cell line has not been done. In the present study different Noni fruit fractions have tested on cancer cell lines MCF-7, MDA-MB-231 (breast adenocarcinoma) and one non-cancer cell line HEK-293 (Human embryonic kidney). Out of which ethylacetate extract showed a higher order of in vitro anticancer activity profile. The ethylacetate extract strongly inhibited the proliferation of MCF-7, MDA-MB-231 and HEK-293 cell lines with IC50 values of 25, 35, 60 µg/ml respectively. The extract showed increase in apoptotic cells in MCF-7 and MDA-MB-231 cells and arrested the cell cycle in the G1/S phase in MCF-7 and G0/G1 phase in MDA-MB-231 cells. Noni extract also decreases the intracellular ROS generation and mitochondrial membrane potential.

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“…The anthraquinone emodin and its derivative, physcion, are major quinones in P. cuspidatum and have potential antimicrobe and anticancer applications (Jayasuriya et al, 1992; Teich et al, 2004; Su et al, 2005; Olsen et al, 2007; Yang et al, 2007; Shan et al, 2008; Narender et al, 2013; Chen et al, 2015; Xiong et al, 2015; Han et al, 2016; Hong et al, 2016; Li et al, 2016; Liu et al, 2016; Pan et al, 2016; Gao et al, 2017). Physcion significantly inhibits cancer cell proliferation and tumor growth in mice by specifically decreasing the activity of 6-phosphogluconate dehydrogenase, thus affecting the oxidative pentose phosphate pathway (Lin et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Assembly and Annotation of a Draft Genome of the Medicinal Plant Polygonum cuspidatum

Zhang

Zheng

et al. 2019

Front. Plant Sci.

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Polygonum cuspidatum (Japanese knotweed, also known as Huzhang in Chinese), a plant that produces bioactive components such as stilbenes and quinones, has long been recognized as important in traditional Chinese herbal medicine. To better understand the biological features of this plant and to gain genetic insight into the biosynthesis of its natural products, we assembled a draft genome of P. cuspidatum using Illumina sequencing technology. The draft genome is ca. 2.56 Gb long, with 71.54% of the genome annotated as transposable elements. Integrated gene prediction suggested that the P. cuspidatum genome encodes 55,075 functional genes, including 6,776 gene families that are conserved in the five eudicot species examined and 2,386 that are unique to P. cuspidatum. Among the functional genes identified, 4,753 are predicted to encode transcription factors. We traced the gene duplication history of P. cuspidatum and determined that it has undergone two whole-genome duplication events about 65 and 6.6 million years ago. Roots are considered the primary medicinal tissue, and transcriptome analysis identified 2,173 genes that were expressed at higher levels in roots compared to aboveground tissues. Detailed phylogenetic analysis demonstrated expansion of the gene family encoding stilbene synthase and chalcone synthase enzymes in the phenylpropanoid metabolic pathway, which is associated with the biosynthesis of resveratrol, a pharmacologically important stilbene. Analysis of the draft genome identified 7 abscisic acid and water deficit stress-induced protein-coding genes and 14 cysteine-rich transmembrane module genes predicted to be involved in stress responses. The draft de novo genome assembly produced in this study represents a valuable resource for the molecular characterization of medicinal compounds in P. cuspidatum, the improvement of this important medicinal plant, and the exploration of its abiotic stress resistance.

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Apoptosis and DNA intercalating activities of novel emodin derivatives

Cited by 15 publications

References 24 publications

Potent Antiproliferative Effect on Liver Cancer of Medicinal Plants Selected from the Thai/Lanna Medicinal Plant Recipe Database “MANOSROI III”

Potent Antiproliferative Effect on Liver Cancer of Medicinal Plants Selected from the Thai/Lanna Medicinal Plant Recipe Database “MANOSROI III”

Anticancer Effects of Extracts from the Fruit of Morinda Citrifolia (Noni) in Breast Cancer Cell Lines

Assembly and Annotation of a Draft Genome of the Medicinal Plant Polygonum cuspidatum

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