Apoptosis and cell cycle arrest of human colorectal cancer cell line HT-29 induced by vanillin

Ho, Ket Li; Yazan, Latifah Saiful; Ismail, Norsharina; Ismail, Muhammad

doi:10.1016/j.canep.2009.06.003

Cited by 137 publications

(88 citation statements)

References 16 publications

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“…Despite the fact that the vanillin concentrations used in this study were exceptionally high, high doses of vanillin were not toxic in healthy rats when administered per gavage or intraperitoneally up to 300 mg/kg . Vanillin is also capable of inducing apoptosis in human colorectal adenocarcinoma (HT-29) cells at a ;2.4-fold lower LC 50 (2.6 mM) than in noncancerous fibroblasts (LC 50 = 6.6 mM in NIH/3T3 cells) because of its concentration-dependent inhibition of cell cycle checkpoints (G 0 /G 1 arrest at IC 50 = 1.3 mM and G 2 /M arrest at IC 50 = 6.2 mM) (Ho et al, 2009).…”

Section: B Pharmacokinetics and Pharmacodynamics Of Curcumin Catabolmentioning

confidence: 99%

The Molecular Basis for the Pharmacokinetics and Pharmacodynamics of Curcumin and Its Metabolites in Relation to Cancer

Golen²,

et al. 2013

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Section: B Pharmacokinetics and Pharmacodynamics Of Curcumin Catabolmentioning

confidence: 99%

The Molecular Basis for the Pharmacokinetics and Pharmacodynamics of Curcumin and Its Metabolites in Relation to Cancer

Golen²,

et al. 2013

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“…The compounds identified were α-amyrin, phytol, vanillin, benzenedicarboxylic acid, squalene, nicotinic acid, campesterol, stigmasterol and α-tocopherol. Vanillin, a widely used flavouring agent, is also reported to successfully inhibit proliferation of HT-29 colon cancer cells by inducing apoptosis (Ho et al, 2009). Studies on phytol report significant cytotoxicity on nasopharyngeal carcinoma (KB) cell line (23 µg mL -1 ) (Malek et al, 2009).…”

Section: Gc-ms Profilingmentioning

confidence: 99%

Phytochemistry, Cytotoxicity and Apoptosis Studies of Β-Sitosterol-3-O-Glucoside and Β -Amyrin From Prunus Africana

Maiyo¹,

Moodley²,

Singh³

2016

Afr J Tradit Complement Altern Med

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Background: Prunus africana is used traditionally in many countries for the treatment of cancer and benign prostate hyperplasia. Materials and methods: In this study, compounds from the leaves and bark of this plant were isolated and tested for their cytotoxicity and apoptosis induction in two human cancer cell lines (hepatocellular carcinoma (HepG2) and colorectal carcinoma (Caco-2)) and a non-cancer cell line (embryonic kidney (HEK293)). GC-MS profiling of the extract was also conducted. Results: Three compounds (β-sitosterol, β-amyrin and β-sitosterol-3-O-glucoside) were isolated and the cytotoxic activity of β-amyrin and β-sitosterol-3-O-glucoside on the HepG2, Caco-2 and HEK293 was determined using the MTT cell viability assay. Both compounds had significant cytotoxic activity towards the Caco-2 cell line with IC50 values of 81 μg mL -1 and 54 μg mL -1 for β-amyrin and β-sitosterol-3-O-glucoside, respectively while low cytotoxicity was observed on HepG2 cell lines with IC50 values of 206 μg mL -1 and 251 μg mL -1 for β-amyrin and β-sitosterol-3-O-glucoside, respectively. Apoptosis induction in cells was studied using acridine orange/ethidium bromide dual staining. In both cases, the compounds tested demonstrated selective cytotoxicity towards cancer cells with high apoptosis indices in cells exposed to β-amyrin. Low IC50 values of 156 μg mL -1 and 937 μg mL -1 for β-amyrin and β-sitosterol-3-O-glucoside, respectively, were observed in the HEK293 cell line. Conclusion: This study reveals that the plant is rich biologically active compounds thereby validating its ethno-medicinal use.

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“…Vanillin has been reported to exhibit multifunctional effects, including antimutagenic, [13][14][15] antiangiogenetic, 16) anti-colitis, 17) anti-sickling, 18) and antianalgesic effects. 19) Recently, we proposed the addition of antioxidant capacity to the multifunctionality of vanillin, 20) but the pharmacological action of ethyl vanillin has been little reported.…”

Section: Contents Of Ethyl Vanillin-and Vanillin-related Compounds Anmentioning

confidence: 99%

Antioxidant Properties of Ethyl Vanillinin Vitroandin Vivo

Tai¹,

Sawano²,

Yazama³

2011

Bioscience, Biotechnology, and Biochemistry

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We systematically evaluated the antioxidant activity of ethyl vanillin, a vanillin analog, as compared with the activities of vanillin and other vanillin analogs using multiple assay systems. Ethyl vanillin and vanillin exerted stronger antioxidant effects than did vanillyl alcohol or vanillic acid in the oxygen radical absorbance capacity (ORAC) assay, although the antioxidant activities of vanillyl alcohol and vanillic acid were clearly superior to those of ethyl vanillin and vanillin in the three model radical assays. The antioxidant activity of ethyl vanillin was much stronger than that of vanillin in the oxidative hemolysis inhibition assay, but was the same as that of vanillin in the ORAC assay. Oral administration of ethyl vanillin to mice increased the concentration of ethyl vanillic acid, and effectively raised antioxidant activity in the plasma as compared to the effect of vanillin. These data suggest that the antioxidant activity of ethyl vanillin might be more beneficial than has been thought in daily health practice.Key words: ethyl vanillin; ABTS radical cation; oxygen radical absorbance capacity (ORAC); oxidative hemolysis inhibition assay; plasma antioxidant activityThe association of reactive oxygen species and free radicals with many disease states is now well recognized, and antioxidants have attracted considerable attention.1,2) It has been reported that there are two types of antioxidants that scavenge radicals quickly and quench many radicals, and it has been proposed that reactivity should be assessed on the basis of both reaction rate and stoichiometry, 3) and that multiple methods should be used, since the activities of some antioxidants vary depending on the assay method.3-6) In addition, comparative studies using common antioxidants appear to be essential to clarify the biological significance of the activities of samples. We have assessed antioxidant activities with attention to the above-mentioned proposals and points of view. We found that 2-O--D-glucopyranosyl-L-ascorbic acid (AA-2G), a stable ascorbic acid derivative, exerted radical-scavenging activity toward unnatural model radicals, including DPPH radical [7][8][9][10] and ABTS radical cation (ABTS þ ). 9,11) The chemical properties of AA-2G as a radical scavenger were widely different from those of ascorbic acid, in that the reaction rate with these model radicals of AA-2G was much slower, but the long-term radical scavenging ability per molecule of AA-2G was superior to that of ascorbic acid. Recently, we reassessed the antioxidant activity of arbutin using five in vitro assay systems, though arbutin has been reported to possess weak antioxidant activity as compared to its precursor, hydroquinone.12) We found that arbutin exerted strong antioxidant activity, comparable or even superior to that of hydroquinone in the ABTS þ -scavenging assay, the ORAC assay, and two cell-based antioxidant assays.Vanillin is widely used in foods, beverages, cosmetics, and drugs. It has been reported to have multifunctional effects, including an...

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Apoptosis and cell cycle arrest of human colorectal cancer cell line HT-29 induced by vanillin

Abstract: Cytolytic and cytostatic effects shown by vanillin showed that it could be a useful colorectal cancer preventive agent. Further in vivo study should be carried out to confirm that similar effects could happen in animals.

Cited by 137 publications

References 16 publications

The Molecular Basis for the Pharmacokinetics and Pharmacodynamics of Curcumin and Its Metabolites in Relation to Cancer

The Molecular Basis for the Pharmacokinetics and Pharmacodynamics of Curcumin and Its Metabolites in Relation to Cancer

Phytochemistry, Cytotoxicity and Apoptosis Studies of Β-Sitosterol-3-O-Glucoside and Β -Amyrin From Prunus Africana

Antioxidant Properties of Ethyl Vanillinin Vitroandin Vivo

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