Apoptosis and autophagy induced by DVDMs-PDT on human esophageal cancer Eca-109 cells

Shi, Yin; Zhang, Boli; Feng, Xiaolan; Qü, Fei; Wang, Shuang; Wu, Linquan; Wang, Xiaobing; Liu, Quanhong; Wang, Pan; Zhang, Kun

doi:10.1016/j.pdpdt.2018.09.013

Cited by 18 publications

(22 citation statements)

References 44 publications

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“…In the study conducted by Huang, et al [ 29 ], the results showed that Pristimerin reduced the growth of esophageal carcinoma. Moreover, previous studies also indicate that ginsenoside Rk3 [ 30 ] and Sinoporphyrin sodium (DVDMs)-Photodynamic therapy (PDT) [ 31 ], which exert their functions by targeting autophagy, inhibit the survival of esophageal carcinoma cells. Furthermore, other studies also illustrate that the treatment targeting autophagy contributes to enhancing the anti-tumor effect [ 32 – 34 ], which functions based on the chemotherapy agents (cisplatin [ 33 ], 5-fluorouracil (5-FU) [ 34 ]).…”

Section: Discussionmentioning

confidence: 99%

Prognostic significance of autophagy-related genes within esophageal carcinoma

Chen

Cao

et al. 2020

BMC Cancer

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Background: Several works suggest the importance of autophagy during esophageal carcinoma development. The aim of the study is to construct a scoring system according to the expression profiles of major autophagy-related genes (ARGs) among esophageal carcinoma cases. Methods: The Cancer Genome Atlas was employed to obtain the esophageal carcinoma data. Thereafter, the online database Oncolnc (http://www.oncolnc.org/) was employed to verify the accuracy of our results. According to our results, the included ARGs were related to overall survival (OS). Results: We detected the expression patterns of ARG within esophageal carcinoma and normal esophageal tissues. In addition, we identified the autophagy related gene set, including 14 genes displaying remarkable significance in predicting the esophageal carcinoma prognosis. The cox regression results showed that, 7 ARGs (including TBK1, ATG5, HSP90AB1, VAMP7, DNAJB1, GABARAPL2, and MAP2K7) were screened to calculate the ARGs scores. Typically, patients with higher ARGs scores were associated with poorer OS. Moreover, the receiver operating characteristic (ROC) curve analysis suggested that, ARGs accurately distinguished the healthy people from esophageal carcinoma patients, with the area under curve (AUC) value of > 0.6. Conclusion: A scoring system is constructed in this study based on the main ARGs, which accurately predicts the outcomes for esophageal carcinoma.

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Section: Discussionmentioning

confidence: 99%

Prognostic significance of autophagy-related genes within esophageal carcinoma

Chen

Cao

et al. 2020

BMC Cancer

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“…Sinoporphyrin sodium (DVDMS) is a novel sensitizer that is activated by both laser and ultrasound. Previous studies have confirmed that DVDMS-mediated photodynamic therapy (PDT) and sonodynamic therapy (SDT) are effective against several types of tumors through mechanisms such as ROS generation, DNA damage, matrix metallic proteinase downregulation, collapse of F-actin filaments and cell apoptosis ( 32 – 34 ). The present study demonstrated the antitumor effects of DVDMS-PDT and DVDMS-SDT in U-118 MG cells in vitro and in vivo .…”

Section: Discussionmentioning

confidence: 99%

“…The role of autophagy in PDT-and SDT-induced cell damage is very complex. Shi et al (34) found that DVDMS-PDT induced apoptosis and autophagy in esophageal cancer cells via ROS generation, while inhibiting autophagy reduced DVDMS-PDT-triggered apoptosis. However, Zhu et al (50) found that inhibiting autophagy with chloroquine enhanced DVDMS-PDT-induced apoptosis in colorectal cancer cells.…”

Section: Discussionmentioning

confidence: 99%

Sinoporphyrin sodium is a promising sensitizer for photodynamic and sonodynamic therapy in glioma

Liu

Zhou

et al. 2020

Oncol Rep

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The aim of the present study was to explore the antitumor effects of sinoporphyrin sodium (DVDMS)-mediated photodynamic therapy (PDT) and sonodynamic therapy (SDT) in glioma, and to reveal the underlying mechanisms. The uptake of DVDMS by U-118 MG cells was detected by flow cytometry (FCM). A 630-nm semiconductor laser and 1-MHz ultrasound were used to perform PDT and SDT, respectively. Cell proliferation and apoptosis were evaluated using the Cell Counting Kit-8 assay, FCM and Hoechst 33258 staining, respectively. Western blot analysis was used to detect protein expression and phosphorylation levels. BALB/c nude mice were used to establish a xenograft model of U-118 MG cells. DVDMS was injected intravenously and PDT and SDT were performed 24 h later. An in vivo imaging system was used to evaluate the fluorescence of DVDMS, to measure tumor sizes, and to evaluate the therapeutic effects. The uptake of DVDMS by U-118 MG cells was optimal after 4 h. PDT and SDT following DVDMS injection significantly inhibited the proliferation and increased apoptosis of glioma cells in vitro (P<0.05, P<0.01) respectively. In vivo, the fluorescence intensity of DVDMS was lower in the PDT and SDT groups compared with the DVDMS group, while tumor cell proliferation and weight were lower in the PDT and SDT groups than in the control group (P<0.05, P<0.01). However, there was no significant difference when laser, ultrasound or DVDMS were applied individually, compared with the control group. Hematoxylin and eosin staining suggested that both PDT and SDT induced significant apoptosis and vascular obstruction in cancer tissues. DVDMS-mediated PDT and SDT inhibited the expression levels of proliferating cell nuclear antigen (PCNA) and Bcl-xL, increased cleaved-caspase 3 levels, and decreased the protein phosphorylation of the PI3K/AKT/mTOR signaling pathway. Changes in the expression of PCNA, and Bcl-xL and in the levels of cleaved-caspase 3 were partly reversed by N-acetyl-L-cysteine, a reactive oxygen species (ROS) scavenger. Similar results were obtained with FCM. DVDMS-mediated PDT and SDT inhibited glioma cell proliferation and induced cell apoptosis in vitro and in vivo, potentially by increasing the generation of ROS and affecting protein expression and phosphorylation levels.

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“…Likewise, Lin et al (2017) showed that HYP-PDT could re-sensitize previously oxaliplatin-resistant colon cancer cells through autophagic cell death mode activation [ 114 ]. Similarly, recent studies have demonstrated that sinoporphyrin sodium (DVDMS) could induce cell death on esophageal cancer cells, through autophagy, which inhibited apoptosis [ 115 , 116 ]. In contrast, Kaizhen et al (2018) showed that autophagy has a pro-survival role, which is abrogated through a dual action of 3-methyladenine (autophagic inhibitor) and Ce6-PDT successfully inducing apoptosis [ 116 ].…”

Section: Introductionmentioning

confidence: 99%

“…Currently, the therapeutic efficacy of HYP-PDT is being tested in a phase III clinical trial (NCT02448381) for the treatment of cutaneous T-cell lymphoma. Of late, Sun et al (2017) demonstrated how rod-shaped gold nanoparticles (AuNRs) and Ce6-doped mesoporous silica nanorods would allow synergistically combining AuNR-induced photothermal therapy (PTT) (increasing tumor temperature above body temperatures of 42–45 °C) and Ce6-activated PDT as demonstrated in pre-clinical animal models [ 115 ]. This latter combined therapy could enable the monitoring of the therapeutic responses using photoacoustic (AuNR) or near-infrared (NIR) imaging (using Ce6) respectively [ 117 ].…”

Section: Introductionmentioning

confidence: 99%

Antibody-Based Immunotherapy: Alternative Approaches for the Treatment of Metastatic Melanoma

et al. 2020

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Melanoma is the least common form of skin cancer and is associated with the highest mortality. Where melanoma is mostly unresponsive to conventional therapies (e.g., chemotherapy), BRAF inhibitor treatment has shown improved therapeutic outcomes. Photodynamic therapy (PDT) relies on a light-activated compound to produce death-inducing amounts of reactive oxygen species (ROS). Their capacity to selectively accumulate in tumor cells has been confirmed in melanoma treatment with some encouraging results. However, this treatment approach has not reached clinical fruition for melanoma due to major limitations associated with the development of resistance and subsequent side effects. These adverse effects might be bypassed by immunotherapy in the form of antibody–drug conjugates (ADCs) relying on the ability of monoclonal antibodies (mAbs) to target specific tumor-associated antigens (TAAs) and to be used as carriers to specifically deliver cytotoxic warheads into corresponding tumor cells. Of late, the continued refinement of ADC therapeutic efficacy has given rise to photoimmunotherapy (PIT) (a light-sensitive compound conjugated to mAbs), which by virtue of requiring light activation only exerts its toxic effect on light-irradiated cells. As such, this review aims to highlight the potential clinical benefits of various armed antibody-based immunotherapies, including PDT, as alternative approaches for the treatment of metastatic melanoma.

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Apoptosis and autophagy induced by DVDMs-PDT on human esophageal cancer Eca-109 cells

Cited by 18 publications

References 44 publications

Prognostic significance of autophagy-related genes within esophageal carcinoma

Prognostic significance of autophagy-related genes within esophageal carcinoma

Sinoporphyrin sodium is a promising sensitizer for photodynamic and sonodynamic therapy in glioma

Antibody-Based Immunotherapy: Alternative Approaches for the Treatment of Metastatic Melanoma

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