Apoptosis and active caspase-3 expression in human granulosa cells

Glamočlija, Violeta; Vilović, Katarina; Saraga-Babić, Mirna; Baranović, Anamarija; Sapunar, Damir

doi:10.1016/j.fertnstert.2004.06.075

Cited by 78 publications

(56 citation statements)

References 33 publications

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“…In IUGR, caspase-3 was also noted in and around nuclei, this may reflect more advanced apoptotic processes as Feng et al describe change in caspase-3 localisation from the membrane to the nucleus as apoptosis progresses in MOLT-4 cells [29]. A similar pattern of caspase-3 localisation is also seen in human ovarian granulosa cells which precedes DNA fragmentation [30]. The localisation of caspase-3 in term placenta coincides with that of poly(ADP-ribose) polymerase (PARP) [28], which could lead to cleavage and inactivation of PARP, precipitating apoptosis.…”

Section: Discussionmentioning

confidence: 71%

Intra-uterine growth restriction is associated with increased apoptosis and altered expression of proteins in the p53 pathway in villous trophoblast

et al. 2010

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Intrauterine growth restriction (IUGR) affects 3-8% of pregnancies and is associated with altered cell turnover in the villous trophoblast, an essential functional cell type of the human placenta. The intrinsic pathway of apoptosis, particularly p53, is important in regulating placental cell turnover in response to damage. We hypothesised that expression of proteins in the p53 pathway in placental tissue would be altered in IUGR. Expression of constituents of the p53 pathway was assessed using real-time PCR, Western blotting and immunohistochemistry. p53 mRNA and protein expression was increased in IUGR, which localised to the syncytiotrophoblast. Similar changes were noted in p21 and Bax expression. There was no change in the expression of Mdm2, Bak and Bcl-2. The association between altered trophoblast cell turnover in IUGR and increased p53 expression is reminiscent of that following exposure to hypoxia. These observations provide further insight into the potential pathogenesis of IUGR. Further research is required to elicit the role and interactions of p53 and its place in the pathogenesis of IUGR.

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Section: Discussionmentioning

confidence: 71%

Intra-uterine growth restriction is associated with increased apoptosis and altered expression of proteins in the p53 pathway in villous trophoblast

et al. 2010

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“…The detection of active caspase-3 and TUNEL are two methods frequently used to study apoptosis in different mammalian species, including humans, in which granulosa cells undergoing cell death positive to caspase-3 was observed. However, few granulosa cells are TUNEL positive in human samples [62].…”

Section: Discussionmentioning

confidence: 96%

Analysis of different cell death processes of prepubertal rat oocytes in vitro

et al. 2010

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The processes of cell death were studied in vitro in populations of oocytes isolated from prepubertal rats. In order to identify apoptosis, the externalized phosphatidylserine was recognized with Annexin-V coupled to FITC and the fragmentation of DNA was demonstrated by means of electrophoresis. Oocytes were tested for autophagy by means of the incorporation of monodansylcadaverine and monitoring Lc3-I/Lc3-II by western blot. The expression of mRNA marker genes of autophagy and of apoptosis was studied by means of RT-PCR in pure populations of oocytes. Some oocytes expressed at least one of the following markers: caspase-3, lamp1 and Lc3. Some oocytes were positive to Annexin-V or to monodansylcadaverine. However, most of them were simultaneously positive to both markers. The relative frequency of oocytes simultaneously positive to markers of apoptosis and autophagy did not change in the different ages studied. The transformation of Lc3-I in Lc3-II was present in all populations of oocytes studied. The mRNAs for caspase-3, lamp1 and Lc3 were present in all populations of oocytes analyzed. Our results demonstrate that oocytes of rats from new born to prepubertal age are eliminated by means of three different cell death processes: apoptosis, autophagy and a mixed event in which both routes to cell death participate in the same cell.

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“…The authors noted that, in contrast to embryonic germ cell death and attrition during nest breakdown, the death of primordial follicles did not seem to occur through classic apoptosis. Similar studies in humans did not confirm the presence of morphological and biochemical apoptotic markers in atretic primordial and primary follicles [14,20]. Tingen et al [15] have postulated that oocytes encapsulated in follicles may similarly eschew apoptosis.…”

Section: Discussionmentioning

confidence: 96%

Follicular atresia in the prepubertal spiny mouse (Acomys cahirinus) ovary

Hułas-Stasiak

Gawron

2011

Apoptosis

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This study was designed to determine follicular atresia in the newborn and the prepubertal spiny mouse. We analyzed the processes of follicle loss using classical markers of apoptosis (TUNEL reaction, active caspase-3) and autophagy (Lamp1). Numerous small clear vacuoles and autophagosomes as well as strong Lamp1 staining were observed in dying oocytes of all follicle types, especially of the primordial and primary ones. Active caspase 3 and the TUNEL reaction were detected only in the granulosa cells of large secondary and antral follicles. The expression of apoptosis and autophagy markers was also changing during the prepubertal period. Western blot analysis indicated that at the moment of birth, females undergo an increased rate of follicular atresia mediated by autophagy, while apoptosis is the dominant form of ovarian atresia in consecutive postnatal days. On the basis of these observations, we concluded that apoptosis and autophagy are involved in follicular atresia and these processes are cell and developmental stage-specific.

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Apoptosis and active caspase-3 expression in human granulosa cells

Cited by 78 publications

References 33 publications

Intra-uterine growth restriction is associated with increased apoptosis and altered expression of proteins in the p53 pathway in villous trophoblast

Intra-uterine growth restriction is associated with increased apoptosis and altered expression of proteins in the p53 pathway in villous trophoblast

Analysis of different cell death processes of prepubertal rat oocytes in vitro

Follicular atresia in the prepubertal spiny mouse (Acomys cahirinus) ovary

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