Apolipoprotein mediates soft-shelled turtle systemic septicemia spherical virus (STSSSV) infection

Chen, Meijuan; Li, Dengfeng; Fang, Jing; Zhang, Liping; Gu, Yehua; Liu, Lianguo; Zhang, Ge

doi:10.1016/j.aquaculture.2017.03.022

Cited by 10 publications

(3 citation statements)

References 22 publications

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“…[4][5][6][7][8][9]. Viruses identified to cause diseases in soft shelled turtles include the Soft-shelled turtle iridovirus (STIV) [10], Trionyx sinensis hemorrhagic syndrome virus (TSHSV) [11] and turtle systemic septicemia spherical virus (TSSSV) [12]. To elucidate the prevalence of these pathogens, there is need to develop rapid diagnostic tools for mass surveillance.…”

Section: Introductionmentioning

confidence: 99%

Immunization with Bovine Serum Albumin (BSA) in Oil-Adjuvant Elicits IgM Antibody Response in Chinese Soft-Shelled Turtle (Pelodiscus Sinensis)

Chen

et al. 2020

Vaccines

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Immunoassays are among the frontline methods used for disease diagnosis and surveillance. Despite this, there are no immunoassays developed for the Chinese soft-shelled turtle (Pelodiscus sinensis), which has expanded into large scale commercial production in several Asian countries. One of the critical factors delaying the development of immunoassays is the lack of characterized soft-shelled turtle immunoglobulins. Herein, we used mass spectrometry together with the ProtQuest software to identify the soft-shelled turtle IgM heavy chain in serum, which again was used to produce a polyclonal anti-turtle-IgM in rabbits. Thereafter, the polyclonal anti-turtle-IgM was used as a secondary antibody in an indirect ELISA to evaluate antibody responses of soft-shelled turtles injected with the bovine serum albumin (BSA) model antigen. Our findings show that only turtle immunized with a water-in-oil BSA plus ISA 763A VG adjuvant (SEPPIC, France) emulsion had antibodies detected at 42 days post vaccination (dpv) while turtles injected with phosphate buffered saline (PBS) only as well as turtle injected with BSA dissolved in PBS had no significant antibody levels detected in serum throughout the study period. In summary, our findings show that rabbit polyclonal anti-turtle-IgM produced can be used in ELISA to measure serum antibody responses in immunized soft-shelled turtles. Future studies should explore its application in other immunoassays needed for the disease diagnosis and vaccine development for soft-shelled turtles.

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Section: Introductionmentioning

confidence: 99%

Immunization with Bovine Serum Albumin (BSA) in Oil-Adjuvant Elicits IgM Antibody Response in Chinese Soft-Shelled Turtle (Pelodiscus Sinensis)

Chen

et al. 2020

Vaccines

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“…Trionyx sinensis hemorrhagic syndrome virus (TSHSV) was first discovered and isolated by Liu in our laboratory in 2013. This virus has recently become an important factor limiting the sustainable development of T. sinensis farming [5,13]. When compared with previously discovered T. sinensis viruses, TSHSV appears to be more virulent, causing high mortality both in the wild and in captivity [5].…”

Section: Introductionmentioning

confidence: 99%

“…This virus has recently become an important factor limiting the sustainable development of T. sinensis farming [5,13]. When compared with previously discovered T. sinensis viruses, TSHSV appears to be more virulent, causing high mortality both in the wild and in captivity [5]. TSHSVinfected turtles display typical characteristics, namely breathing difficulty, interstitial pneumonia, and hyperemic laryngeal mucosa in the throat [13,14].…”

Section: Introductionmentioning

confidence: 99%

Complete genome sequence and analysis of a new lethal arterivirus, Trionyx sinensis hemorrhagic syndrome virus (TSHSV), amplified from an infected Chinese softshell turtle

et al. 2019

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Trionyx sinensis hemorrhagic syndrome virus (TSHSV) is a newly discovered lethal arterivirus that causes serious disease in Trionyx sinensis in China. In this study, the complete genome sequence of TSHSV was determined by RACE cloning, and the functions of the predicted proteins were predicted. The complete genome of TSHSV was found to be 17,875 bp in length, and a 3'-end poly(A) tail was detected. Eight TSHSV hypothetical proteins (TSHSV-HPs) were predicted by gene model identification. TSHSV-HP2, 3 and 4 were associated with replicase activity, since papain-like protease (PLPs), serinetype endopeptidase, P-loop-containing nucleoside triphosphate hydrolase, and EndoU-like endoribonuclease motifs were detected. Phylogenetic analysis showed that TSHSV clusters with an arterivirus from a Chinese broad-headed pond turtle.Publisher's Note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

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Molecular detection of novel herpesviruses and adenoviruses in two species of Australian freshwater turtles

et al. 2023

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Cutaneous lesions were observed in a wild population of freshwater turtles during routine disease surveillance at Alligator Creek, Townsville, Australia. Previous attempts to identify the causative agent of these lesions were unsuccessful; however, existing evidence suggests viral etiology. To further investigate these events, blood samples and lesion, oral and cloacal swabs collected from 128 freshwater turtles at two locations (Alligator Creek and Ross River) were screened for herpesvirus, adenovirus, poxvirus and papillomavirus by polymerase chain reaction. We detected three novel herpesviruses (chelid herpesvirus 1–3) as well as three adenoviruses (saw-shelled turtle adenovirus 1–3). Phylogenetic analyses showed that the herpesviruses formed a distinct clade with the tumour-associated chelonid alphaherpesvirus 5 (Scutavirus chelonidalpha5) within the subfamily Alphaherpesvirinae. The adenoviruses clustered with members of the genus Testadenovirus. Although the novel herpesviruses and adenoviruses could not be linked to the occurrence of cutaneous lesions, further characterisation will help provide better insights into their clinical, epidemiological and conservation significance.

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Apolipoprotein mediates soft-shelled turtle systemic septicemia spherical virus (STSSSV) infection

Cited by 10 publications

References 22 publications

Immunization with Bovine Serum Albumin (BSA) in Oil-Adjuvant Elicits IgM Antibody Response in Chinese Soft-Shelled Turtle (Pelodiscus Sinensis)

Immunization with Bovine Serum Albumin (BSA) in Oil-Adjuvant Elicits IgM Antibody Response in Chinese Soft-Shelled Turtle (Pelodiscus Sinensis)

Complete genome sequence and analysis of a new lethal arterivirus, Trionyx sinensis hemorrhagic syndrome virus (TSHSV), amplified from an infected Chinese softshell turtle

Molecular detection of novel herpesviruses and adenoviruses in two species of Australian freshwater turtles

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