Apolipoprotein E, CI and B Gene Polymorphisms in a Sample of Patients with Coronary Heart Disease in the Kuwaiti Population

Abstract

Objectives: The objective of this study was to investigate the possible association of clinical variables and apolipoprotein (APOE, APOCI and APOB) polymorphisms with the development of myocardial infraction (MI) and coronary heart disease (CHD) in Kuwaitis. Subjects and Methods: APOE, APOCI and APOB genotypes were determined by polymerase chain reaction followed by restriction fragment length polymorphism in 143 Kuwaiti CHD patients with (n = 88) and without (n = 55) MI and in 122 controls matched for gender and age. Statistical and genetic analyses of the genotype, allele and haplotype frequencies, as well as regression analyses of genetic and clinical variables were done. Results: There was a statistically significant association between CHD and medical history of diabetes mellitus (p < 0.001), hypertension (p < 0.01), high cholesterol (p < 0.05) and family history of CHD (p < 0.001). A highly significant association (p < 0.001) was found, with an adjusted odds ratio of 9.32, for family history and the development of MI. No significant differences were found for allele or genotype frequencies between CHD patients and controls. Conclusion: The strong effect of family history suggests a major genetic component for the development of CHD in Kuwaitis, but this association does not appear to be related to the APO genes studied here. The results in this study encourages future research into these and other polymorphisms and their potential association with MI and CHD in the Kuwaiti population.

Apolipoprotein E Gene Variants and Risk of Coronary Heart Disease: A Meta-Analysis

“…All studies indicated that the distribution of genotypes in the controls was consistent with HWE, except for 7 studies [29, 35, 37, 39, 40, 45, 46] ( P < 0.05). According to the quality criteria, there were 21 studies with high quality (NOS score > 6).…”

Association between Apolipoprotein E polymorphism and myocardial infarction risk: A systematic review and meta‐analysis

“…Based on the search strategy, 571 potentially eligible studies were identified in the initial search. Among these, 22 studies were enrolled in this meta-analysis based on the inclusion criteria [11] , [12] , [13] , [14] , [15] , [16] , [17] , [18] , [19] , [20] , [21] , [22] , [23] , [24] , [25] , [26] , [27] , [28] , [29] , [30] , [31] , [32] ( Fig. 1 ).…”

Meta-Analysis of Apolipoprotein E Gene Polymorphism and Susceptibility of Myocardial Infarction

“…Therefore, further studies are warranted among Asian populations. In addition, genotype distributions in the controls from Scaglione’s study [28], Bustan’s study [49] and Zende’s study [52] were not in agreement with HWE, therefore, the results may be biased. However, sensitivity analysis suggested that the pooled results were not significantly changed after excluding the three studies (data not shown).…”