Apolipoprotein D is down-regulated during malignant transformation of neurofibromas

Hunter, Stephen B.; Weiss, Sharon W.; Ou, Chen Yih; Jaye, David L.; Young, Andrew N.; Wilcox, Josiah N.; Arbiser, Jack L.; Monson, David K.; Goldblum, John R.; Nolen, John David L.; Varma, Vijay

doi:10.1016/j.humpath.2005.06.018

Cited by 11 publications

(6 citation statements)

References 45 publications

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“…Cell culture studies revealed that APOD transcription in fibroblasts is induced by growth arrest and thus specifically seen in nonproliferating quiescent and senescent cells 23, 24. Our finding that APOD expression decreases with astrocytoma progression is in line with recent studies reporting on APOD downregulation with increasing malignancy in colon cancers25 and peripheral nerve sheath tumors 26. In addition, low levels of apolipoprotein D expression were found to be associated with shorter survival of patients with breast cancer27 and colon cancer 25.…”

Section: Discussionsupporting

confidence: 89%

Identification of novel genes associated with astrocytoma progression using suppression subtractive hybridization and real‐time reverse transcription‐polymerase chain reaction

Boom

Wolter

Blaschke

et al. 2006

Intl Journal of Cancer

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To identify novel genes involved in glioma progression we performed suppression subtractive hybridization combined with cDNA array analysis on 4 patients with primary low-grade gliomas of World Health Organization (WHO) grade II that recurred as secondary glioblastomas (WHO grade IV). Eight genes showing differential expression between primary and recurrent tumors in 3 of the 4 patients were selected for further analysis using realtime reverse transcription-PCR on a series of 10 pairs of primary low-grade and recurrent high-grade gliomas as well as 42 astrocytic gliomas of different WHO grades. These analyses revealed that 5 genes, i.e., AMOG (ATP1B2, 17p13.1), APOD (3q26.2-qter), DMXL1 (5q23.1) DRR1 (TU3A, 3p14.2) and PSD3 (KIAA09428/ HCA67/EFA6R, 8p22), were expressed at significantly lower levels in secondary glioblastomas as compared to diffuse astrocytomas of WHO grade II. In addition, AMOG, DRR1 and PSD3 transcript levels were significantly lower in primary glioblastomas than in diffuse astrocytomas. Treatment of glioma cell lines with 5-aza-2 0 -deoxycytidine and trichostatin A resulted in increased expression of AMOG and APOD transcripts. Sequencing of sodium bisulfitemodified DNA demonstrated AMOG promoter hypermethylation in the glioma cell lines and 1 primary anaplastic astrocytoma with low AMOG expression. Taken together, we identified interesting novel candidate genes that likely contribute to glioma progression and provide first evidence for a role of epigenetic silencing of AMOG in malignant glioma cells. ' 2006 Wiley-Liss, Inc.Key words: adhesion molecule on glia; astrocytoma; glioblastoma; suppression subtractive hybridization; tumor progression Diffuse astrocytomas of World Health Organization (WHO) grade II are characterized by an intrinsic tendency to local recurrence and spontaneous progression to high grade anaplastic astrocytomas (WHO grade III) or secondary glioblastomas (WHO grade IV).1 The molecular mechanisms underlying this malignant progression are still poorly understood. Diffuse astrocytomas of WHO grade II commonly carry TP53 mutations, show loss of heterozygosity (LOH) on 17p and have gained 1 copy of chromosome 7 or 7q.2,3 TP53 mutations and gains on chromosome 7 are similarly frequent in anaplastic astrocytomas of WHO grade III but these tumors often carry additional alterations, such as deletions on chromosomes 6, 9p, 11p, 19q and 22q. Furthermore, a subset of anaplastic astrocytomas shows genetic alterations of the cell cycle regulatory genes CDKN2A, CDKN2B, CDK4 or RB1. 2,3Glioblastomas, the most common and most malignant astrocytic gliomas, are characterized by complex genomic aberrations causing the inactivation of various tumor suppressor genes as well as the activation of different proto-oncogenes.2,3 According to the patient's clinical history, 2 types of glioblastomas can be distinguished. More common are primary glioblastomas that arise de novo with a short clinical history and without a preexisting glioma of lower malignancy grade. Less common are secondary glioblas...

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Section: Discussionsupporting

confidence: 89%

Identification of novel genes associated with astrocytoma progression using suppression subtractive hybridization and real‐time reverse transcription‐polymerase chain reaction

Boom

Wolter

Blaschke

et al. 2006

Intl Journal of Cancer

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“…An extensive literature supports the notion that tumor cells maintain low levels of ApoD in their surroundings, and a decreased mRNA and/or protein expression has been reported in breast [22], ovary [23], prostate [24], hepatic [25,26], neural [27,28], esophageal cancer [29], and colorectal cancer [30]. Our data show a prominent decrease in mRNA and protein levels in the initial stages of CRC.…”

Section: Oxidation State Of Crc Along Stages and Its Relationship To supporting

confidence: 89%

“…Many reports document low levels of ApoD in different forms of cancer [22][23][24][25][26][27][28][29]. In primary colorectal cancer, Ogawa et al [30] corroborated a similar downregulation of ApoD messenger RNA (mRNA), and by establishing two categories of mRNA expression, they found a positive correlation between ApoD levels and CRC patient survival.…”

Section: Apod Expression Levels In Crc Progressionmentioning

confidence: 99%

Expression and potential role of apolipoprotein D on the death–survival balance of human colorectal cancer cells under oxidative stress conditions

Bajo‐Grañeras

Crespo‐Sanjuán

García-Centeno

et al. 2013

Int J Colorectal Dis

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“…This is in agreement with earlier studies showing that APOD mRNA levels were increased in neurofibroma compared to non-neoplastic peripheral nerve [19]. Interestingly, the same group showed that APOD mRNA levels decreases during transformation of benign neurofibroma to MPNSTs.…”

Section: Resultssupporting

confidence: 93%

Secretome Survey of Human Plexiform Neurofibroma Derived Schwann Cells Reveals a Secreted form of the RARRES1 Protein

Chen

Seol²,

Brown³

et al. 2012

IJMS

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To bring insights into neurofibroma biochemistry, a comprehensive secretome analysis was performed on cultured human primary Schwann cells isolated from surgically resected plexiform neurofibroma and from normal nerve tissue. Using a combination of SDS-PAGE and high precision LC-MS/MS, 907 proteins were confidently identified in the conditioned media of Schwann cell cultures combined. Label free proteome profiling revealed consistent release of high levels of 22 proteins by the four biological replicates of NF1 Schwann cell cultures relative to the two normal Schwann cell cultures. Inversely, 9 proteins displayed decreased levels in the conditioned media of NF1 relative to normal Schwann cells. The proteins with increased levels included proteins involved in cell growth, angiogenesis and complement pathway while proteins with decreased levels included those involved in cell adhesion, plasminogen pathway and extracellular matrix remodeling. Retinoic acid receptor responder protein-1 (RARRES1), previously described as an integral membrane tumor suppressor, was found exclusively secreted by NF1 Schwann cells but not by normal Schwann cells. All-trans retinoic acid modulated secretion of RARRES1 in a dose dependent manner. This study shows altered secretion of key proteins in NF1 derived Schwann cells. The potential implication of these proteins in neurofibroma biology is discussed.

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Apolipoprotein D is down-regulated during malignant transformation of neurofibromas

Cited by 11 publications

References 45 publications

Identification of novel genes associated with astrocytoma progression using suppression subtractive hybridization and real‐time reverse transcription‐polymerase chain reaction

Identification of novel genes associated with astrocytoma progression using suppression subtractive hybridization and real‐time reverse transcription‐polymerase chain reaction

Expression and potential role of apolipoprotein D on the death–survival balance of human colorectal cancer cells under oxidative stress conditions

Secretome Survey of Human Plexiform Neurofibroma Derived Schwann Cells Reveals a Secreted form of the RARRES1 Protein

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