Apolipoprotein A-I, an antimicrobial protein in Oncorhynchus mykiss: Evaluation of its expression in primary defence barriers and plasma levels in sick and healthy fish

Villarroel, Franz; Bastías, Adriana; Casado, A.; Amthauer, Rodolfo; Concha, Miguel

doi:10.1016/j.fsi.2006.10.008

Cited by 107 publications

(68 citation statements)

References 42 publications

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“…In mammals, a group of nine exchangeable and soluble apolipoproteins including apoC-I, apoC-II, apoC-III, apoC-IV, apoA-I, apoA-II, apoA-IV, apoA-V, and apoE is associated with plasma lipoproteins and the structural organization of these genes is thoroughly understood [2,43,44]. Some fish apolipoproteins have been reported to have non-lipidmediated activities such as antimicrobial functions and innate immunity [11][12][13] but the structural organization of apolipoprotein genes in fish is currently limited to a very few studies [4,42]. The genetic information of fish apolipoproteins is expected to contribute not only to a better understanding of apolipoprotein evolution, but to lipid metabolism and physiology in fish lineages.…”

Section: Discussionmentioning

confidence: 99%

“…Clinical and epidemiological studies have demonstrated an inverse correlation between the concentration of HDL-cholesterol and cardiovascular disease [7][8][9][10]. Additionally, fish apoA-I has antimicrobial and antibacterial functions [11,12], and is also an important effector of innate immunity in teleosts [13]. Therefore, the structure and functions of apoA-I gene are very important for the deeper understanding of the physiology of fish.…”

Section: Introductionmentioning

confidence: 99%

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Isolation and characterization of some novel genes of the apolipoprotein A-I family in Japanese eel, Anguilla japonica

et al. 2011

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Apolipoproteins such as apolipoprotein (apo) A-I, apoA-IV, and apoE are lipid binding proteins synthesized mainly in the liver and the intestine and play an important role in the transfer of exogenous or endogenous lipids through the circulatory system. To investigate the mechanism of lipid transport in fish, we have isolated some novel genes of the apoA-I family, apoIA-I (apoA-I isoform) 1–11, from Japanese eel by PCR amplification. Some of the isolated genes of apoIA-I corresponded to 28kDa-1 cDNAs which had already been deposited into the database and encoded an apolipoprotein with molecular weight of 28 kDa in the LDL, whereas others seemed to be novel genes. The structural organization of all apoIA-Is consisted of four exons separated by three introns. ApoIA-I10 had a total length of 3232 bp, whereas other genes except for apoIA-I9 ranged from 1280 to 1441 bp. The sequences of apoIA-Is at the exon-intron junctions were mostly consistent with the consensus sequence (GT/AG) at exon-intron boundaries, whereas the sequences of 3′ splice acceptor in intron 1 of apoIA-I1-7 were (AC) but not (AG). The deduced amino acid sequences of all apoIA-Is contained a putative signal peptide and a propeptide of 17 and 5 amino acid residues, respectively. The mature proteins of apoIA-I1-3, 7, and 8 consisted of 237 amino acids, whereas those of apoIA-I4-6 consisted of 239 amino acids. The mature apoIA-I10 sequence showed 65% identity to amino acid sequence of apoIA-I11 which was associated with an apolipoprotein with molecular weight of 23 kDa in the VLDL. All these mature apoIA-I sequences satisfied the common structural features depicted for the exchangeable apolipoproteins such as apoA-I, apoA-IV, and apoE but apoIA-I11 lacked internal repeats 7, 8, and 9 when compared with other members of apoA-I family. Phylogenetic analysis showed that these novel apoIA-Is isolated from Japanese eel were much closer to apoA-I than apoA-IV and apoE, suggesting new members of the apoA-I family.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Isolation and characterization of some novel genes of the apolipoprotein A-I family in Japanese eel, Anguilla japonica

et al. 2011

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“…It has been shown to be a major bacteriocidic factor in several species of fi sh ( 62 ) as well as humans ( 63,64 ). HDL can also deactivate particular oxidized phospholipids accumulating in macrophages that have been infected with Mycobacterium leprae , the organism that causes leprosy ( 65 ).…”

Section: Hdl Functional Diversity Matches Its Proteomic Diversitymentioning

confidence: 99%

Proteomic diversity of high density lipoproteins: our emerging understanding of its importance in lipid transport and beyond

Shah

Tan

Long

et al. 2013

Journal of Lipid Research

322

302

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“…ApoA-I, the major protein component of high-density lipoprotein, is a multifunctional apolipoprotein that, besides its contribution in cholesterol transport and metabolism, plays important defensive functions in mammals and lower vertebrates. Experiments have shown that the expression level of ApoA-I decreased in duck liver at 8 h and 24 h after R. anatipestifer infection but its expression was significantly up-regulated at 48 h, which is contrary to mammals, in which the hepatic ApoA-I expression is down-regulated under pro-inflammatory conditions, and with fish, in which no significant differences were found in gene expression between livers of healthy and diseased fish (Carpintero et al, 2005;Villarroel et al, 2007); these results suggest perhaps a small functional difference among these species. TTR, also known as thyroxinbinding prealbumin, is a serum protein that is a negative acute-phase reactant in humans (Ingenbleek & Young, 1994).…”

Section: Discussionmentioning

confidence: 99%

Gene expression responses toRiemerella anatipestiferinfection in the liver of ducks

Zhou

Xiao

et al. 2013

Avian Pathology

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Riemerella anatipestifer is one of the most economically important pathogens of farm ducks worldwide. The molecular mechanisms that underlie its pathogenesis, particularly the host response to R. anatipestifer infection, are poorly understood. The differentially expressed gene profile of duck livers at 24 h following R. anatipestifer infection was therefore investigated using suppression subtractive hybridizaton analysis. A total of 45 differentially expressed genes were identified, which primarily included genes for proteins involved in acute-phase response, inflammatory response, immune response, wound healing and iron homeostasis. For the expression level of 20 genes from those 45 analysed by quantitative reverse transcriptase-polymerase chain reaction at 8, 24 and 48 h post infection, significant differences were observed among the three time points of measurements. The result from this study revealed a gene expression profile of duck liver during R. anatipestifer infection, and those genes with a role in the immune response and wound healing deserving further investigation to elucidate their respective roles during infection.

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Apolipoprotein A-I, an antimicrobial protein in Oncorhynchus mykiss: Evaluation of its expression in primary defence barriers and plasma levels in sick and healthy fish

Cited by 107 publications

References 42 publications

Isolation and characterization of some novel genes of the apolipoprotein A-I family in Japanese eel, Anguilla japonica

Isolation and characterization of some novel genes of the apolipoprotein A-I family in Japanese eel, Anguilla japonica

Proteomic diversity of high density lipoproteins: our emerging understanding of its importance in lipid transport and beyond

Gene expression responses toRiemerella anatipestiferinfection in the liver of ducks

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