Apolipophorin‐III‐like protein expressed in the antenna of the red imported fire ant, <i>Solenopsis invicta</i> Buren (Hymenoptera: Formicidae)

Guntur, Kalyani V P; Velasquez, Daniel; Chadwell, Linda V.; Carroll, Christopher A.; Weintraub, Susan E; Cassill, J.Aaron; Renthal, Robert

doi:10.1002/arch.20019

Cited by 9 publications

(12 citation statements)

References 30 publications

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“…In fact, the use of OBPs in ant chemosensation has been questioned. Previous studies were unable to identify any members of this protein family in ant antennae [37], [38], [39], which led Calvello et al [7] to speculate that ants may prefer to use CSPs instead of OBPs for olfaction, which could explain the expansion of CSPs in S. invicta . More recently however, three OBPs have been documented [5], [6, R. Renthal personal communication] in the antennae of red imported fire ant workers (SiOBP15 [OBP1 of Wang et al [7]], SiOBP3 [ GP-9 ], and SiOBP2).…”

Section: Discussionmentioning

confidence: 99%

Odorant Binding Proteins of the Red Imported Fire Ant, Solenopsis invicta: An Example of the Problems Facing the Analysis of Widely Divergent Proteins

et al. 2011

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We describe the odorant binding proteins (OBPs) of the red imported fire ant, Solenopsis invicta, obtained from analyses of an EST library and separate 454 sequencing runs of two normalized cDNA libraries. We identified a total of 18 putative functional OBPs in this ant. A third of the fire ant OBPs are orthologs to honey bee OBPs. Another third of the OBPs belong to a lineage-specific expansion, which is a common feature of insect OBP evolution. Like other OBPs, the different fire ant OBPs share little sequence similarity (∼20%), rendering evolutionary analyses difficult. We discuss the resulting problems with sequence alignment, phylogenetic analysis, and tests of selection. As previously suggested, our results underscore the importance for careful exploration of the sensitivity to the effects of alignment methods for data comprising widely divergent sequences.

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Section: Discussionmentioning

confidence: 99%

Odorant Binding Proteins of the Red Imported Fire Ant, Solenopsis invicta: An Example of the Problems Facing the Analysis of Widely Divergent Proteins

et al. 2011

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“…2D gel electrophoresis and N‐terminal sequencing were carried out as previously described (Guntur et al. , 2004).…”

Section: Methodsmentioning

confidence: 99%

“…Antennal extracts (Guntur et al ., 2004) or purified recombinant CSP1 fractions were unfolded in 8 M guanidinium containing 5 mM dithiothreitol at 60 °C for 1 h followed by treatment with 20 mM iodoacetamide. After addition of excess dithiothreitol and sixfold dilution with buffer, the protein was hydrolysed with trypsin (Gold, mass spectrometry grade, Promega, Madison, WI, USA; 1:20 w : w) for 1 h at 37 °C.…”

Section: Methodsmentioning

confidence: 99%

The major antennal chemosensory protein of red imported fire ant workers

González

Zhao

McMahan

et al. 2009

Insect Molecular Biology

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Some chemosensory proteins (CSPs) are expressed in insect sensory appendages and are thought to be involved in chemical signaling by ants. We identified fourteen unique CSP sequences in EST libraries of the red imported fire ant, Solenopsis invicta. One member of this group (Si-CSP1) is highly expressed in worker antennae, suggesting an olfactory function. A shotgun proteomic analysis of antennal proteins confirms the high level of Si-CSP1 expression, and also shows expression of another CSP and two odorant-binding proteins (OBPs). We cloned and expressed the coding sequence for Si-CSP1. We used cyclodextrins as solubilizers to investigate ligand binding. Fire ant cuticular lipids strongly inhibit Si-CSP1 binding to the fluorescent dye N-phenyl-naphthylamine, suggesting cuticular substances are ligands for Si-CSP1. Analysis of the cuticular lipids shows that the endogenous ligands of Si-CSP1 are not cuticular hydrocarbons.

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“…Several are expressed not in chemosensory organs, but in such sites as the hemolymph (Graham et al 2001;Paskewitz and Shi 2005), male accessory gland (Paesen and Happ 1995), and other tissues devoid of known chemosensory structures (Galindo and Smith 2001;Hekmat-Scafe et al 2002). In S. invicta, GP-9 is present in the hemolymph of adult females (Dietrich Gotzek, personal observation), while it is not expressed in the antennae of males (Guntur et al 2004…”

Section: Gp-9 Is Functionally Importantmentioning

confidence: 99%

Genetic Regulation of Colony Social Organization in Fire Ants: An Integrative Overview

Gotzek¹,

Ross²

2007

The Quarterly Review of Biology

131

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Expression of colony social organization in fire ants appears to be under the control of a single Mendelian factor of large effect. Variation in colony queen number in Solenopsis invicta and its relatives is associated with allelic variation at the gene Gp-9, but not with variation at other unlinked genes; workers regulate queen identity and number on the basis of Gp-9 genotypic compatibility. Nongenetic factors, such as prior social experience, queen reproductive status, and local environment, have negligible effects on queen numbers which illustrates the nearly complete penetrance of Gp-9. As predicted, queen number can be manipulated experimentally by altering worker Gp-9 genotype frequencies. The Gp-9 allele lineage associated with polygyny in South American fire ants has been retained across multiple speciation events, which may signal the action of balancing selection to maintain social polymorphism in these species. Moreover, positive selection is implicated in driving the molecular evolution of Gp-9 in association with the origin of polygyny. The identity of the product of Gp-9 as an odorant-binding protein suggests plausible scenarios for its direct involvement in the regulation of queen number via a role in chemical communication. While these and other lines of evidence show that Gp-9 represents a legitimate candidate gene of major effect, studies aimed at determining (i) the biochemical pathways in which GP-9 functions; (ii) the phenotypic effects of molecular variation at Gp-9 and other pathway genes; and (iii) the potential involvement of genes in linkage disequilibrium with Gp-9 are needed to elucidate the genetic architecture underlying social organization in fire ants. Information that reveals the links between molecular variation, individual phenotype, and colony-level behaviors, combined with behavioral models that incorporate details of the chemical communication involved in regulating queen number, will yield a novel integrated view of the evolutionary changes underlying a key social adaptation.

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Apolipophorin‐III‐like protein expressed in the antenna of the red imported fire ant, Solenopsis invicta Buren (Hymenoptera: Formicidae)

Cited by 9 publications

References 30 publications

Odorant Binding Proteins of the Red Imported Fire Ant, Solenopsis invicta: An Example of the Problems Facing the Analysis of Widely Divergent Proteins

Odorant Binding Proteins of the Red Imported Fire Ant, Solenopsis invicta: An Example of the Problems Facing the Analysis of Widely Divergent Proteins

The major antennal chemosensory protein of red imported fire ant workers

Genetic Regulation of Colony Social Organization in Fire Ants: An Integrative Overview

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