Apoferritin Modified Magnetic Particles as Doxorubicin Carriers for Anticancer Drug Delivery

Blažková, Iva; Nguyen, Hoai Viet; Dostálová, Simona; Kopel, Pavel; Stanisavljević, Maja; Vaculovičová, Markéta; Stiborová, Marie; Eckschlager, Tomáš; Kizek, René; Adam, Vojtěch

doi:10.3390/ijms140713391

Cited by 57 publications

(38 citation statements)

References 31 publications

(30 reference statements)

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“…The delivery of anticancer drugs using apoferritin as a nanocarrier can lower the negative side effects of these drugs, such as the cardiotoxicity of doxorubicin [79] or seizures caused by cisplatin administration [128]. With water-soluble apoferritin, it is possible to dissolve drugs that are normally insoluble in water [7].…”

Section: Delivery Of Anticancer Drugsmentioning

confidence: 99%

“…Since apoferritin can be loaded with a wide spectrum of molecules via the disassembly/reassembly process [79], it is also possible to use apoferritin as a carrier for the delivery of genes. It is also a molecule naturally found in the human body and it possesses many of the abovementioned criteria making it suitable as a gene nanocarrier [18].…”

Section: Apoferritin In Gene Deliverymentioning

confidence: 99%

“…The surface of apoferritin can also be modified with biotin and connected to streptavidin-coated magnetic nano-or microparticles to help guide the movement of apoferritin through the patient's body using an external magnetic field and to combine the targeted drug delivery with hyperthermic therapy [79]. Photosensitisers can be encapsulated within apoferritin for the combination of photodynamic therapy and chemotherapy [140].…”

Section: Delivery Of Anticancer Drugsmentioning

confidence: 99%

“…The spherical cage in apoferritin can be used for the encapsulation of fluorescent dyes [77], drugs [78][79][80][81][82][83][84][85], magnetic resonance imaging contrast agents [86,87], genes [88] or other compounds [89]. Due to the self-assembly activity of apoferritin and its response to the surrounding environment, it has several advantages for use as a nanocarrier.…”

Section: Apoferritin As a Drug / Gene Nanocarriermentioning

confidence: 99%

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Apoferritin: protein nanocarrier for targeted delivery

Dostálová¹,

Heger²,

Kudr³

et al. 2015

Nano Based Drug Delivery

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Section: Delivery Of Anticancer Drugsmentioning

confidence: 99%

Section: Apoferritin In Gene Deliverymentioning

confidence: 99%

Section: Delivery Of Anticancer Drugsmentioning

confidence: 99%

Section: Apoferritin As a Drug / Gene Nanocarriermentioning

confidence: 99%

See 2 more Smart Citations

Apoferritin: protein nanocarrier for targeted delivery

Dostálová¹,

Heger²,

Kudr³

et al. 2015

Nano Based Drug Delivery

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“…This feature allows the application of AFn as a drug nanocarrier with specific low pH-initiated release. 19 It is obvious that AFn (DOX) nanoparticles for tumor-specific drug delivery have an advantage over active pharmaceutical ingredients in tumor inhibition and in vivo toxicity. 20 In this study, AFn (DOX) nanoparticles were labeled with fluorescent and magnetic Gd-CDs for bimodal (MRI/optical) imaging.…”

Section: Introductionmentioning

confidence: 99%

Construction of magnetic-carbon-quantum-dots-probe-labeled apoferritin nanocages for bioimaging and targeted therapy

Yao¹,

Su²,

Zeng³

et al. 2016

IJN

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Carbon dots (CDs) are one of the most highlighted carbon-based materials for biological applications, such as optical imaging nanoprobes, which are used for labeling cells in cancer treatment mainly due to their biocompatibility and unique optical properties. In this study, gadolinium (Gd)-complex-containing CDs were obtained through a one-step microwave method to develop multimodal nanoprobes integrating the advantages of optical and magnetic imaging. The obtained Gd-CDs exhibited highly fluorescent properties with excellent water solubility and biological compatibility. Natural apoferritin (AFn) nanocages, an excellent drug delivery carrier, are hollow in structure, with their pH-dependent, unfolding–refolding process at pH 2.0 and 7.4. The chemotherapeutic drug doxorubicin (DOX) can be highly effective and encapsulated into AFn cavity. A widely used tumor-targeting molecule, folic acid (FA), functionalized the surface of AFn to obtain an active tumor targeting effect on MCF-7 cells and malignant tumors in mice models. In this study, an AFn nanocarrier encapsulating high concentration of DOX labeled with magnetic and fluorescent Gd-CDs probe was developed. Gd-CDs exhibited a unique green photoluminescence and almost no toxicity compared with free GdCl 3 . Furthermore, Gd-doped CDs significantly increased the circulation time and decreased the toxicity of Gd 3+ in in vitro and in vivo magnetic resonance imaging, which demonstrated that the AFn nanocages labeled with Gd-CD compounds could serve as an excellent T 1 contrast agent for magnetic resonance imaging. The self-assembling multifunctional Gd-CDs/AFn (DOX)/FA nanoparticles have a great potential for cancer theranostic applications.

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Encapsulation of the Dinuclear Trithiolato‐Bridged Arene Ruthenium Complex Diruthenium‐1 in an Apoferritin Nanocage: Structure and Cytotoxicity

et al. 2019

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The effects of encapsulating the cytotoxic dinuclear trithiolato‐bridged arene ruthenium complex [(η6‐p‐MeC6H4iPr)2Ru2(μ2‐S‐p‐C6H4tBu)3]Cl (DiRu‐1) within the apoferritin (AFt) nanocage were investigated. The DiRu‐1‐AFt nanocarrier was characterized by UV/Vis spectroscopy, ICP‐MS, CD and X‐ray crystallography. In contrast to previously reported Au‐ and Pt‐based drug‐loaded AFt carriers, we found no evidence of direct interactions between DiRu‐1 and AFt. DiRu‐1‐AFt is cytotoxic toward immortalized murine BALB/c‐3T3 fibroblasts transformed with SV40 virus (SVT2) and human epidermoid carcinoma A431 malignant cells, and exhibits moderate selectivity for these cancer cells over normal BALB/c‐3T3 cells. DiRu‐1‐AFt triggers the production of reactive oxygen species, depolarization of mitochondrial membrane potential, and induces cell death via p53‐mediated apoptosis. Comparison between our data and previous results suggests that the presence of specific interactions between a metal‐based drug and AFt within the protein cage is not essential for drug encapsulation.

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Apoferritin Modified Magnetic Particles as Doxorubicin Carriers for Anticancer Drug Delivery

Cited by 57 publications

References 31 publications

Apoferritin: protein nanocarrier for targeted delivery

Apoferritin: protein nanocarrier for targeted delivery

Construction of magnetic-carbon-quantum-dots-probe-labeled apoferritin nanocages for bioimaging and targeted therapy

Encapsulation of the Dinuclear Trithiolato‐Bridged Arene Ruthenium Complex Diruthenium‐1 in an Apoferritin Nanocage: Structure and Cytotoxicity

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