Apocrine secretion of amylase and exocytosis of trypsin along the midgut of Tenebrio molitor larvae

Cristofoletti, Plı́nio T.; Ribeiro, Alberto F.; Terra, Walter R.

doi:10.1016/s0022-1910(00)00098-6

Cited by 122 publications

(103 citation statements)

References 34 publications

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“…These authors suggested that the phenomenon of cytoplasm loss is probably correlated to cell degeneration in epithelial renewal. These patterns occur due to high enzyme secretion activity in the midgut in an attempt to restore the lining that is being attacked (Terra & Ferreira 1994;Cristofoletti et al 2000;Levy et al 2004). Billingsley & Lehane (1996) and Levy et al (2004) report morphological aspects similar to those of S. frugiperda regenerative cells that are responsible for the process of lining cell recovery.…”

Section: Resultsmentioning

confidence: 94%

The effect of sub-lethal doses of Azadirachta indica (Meliaceae) oil on the midgut of Spodoptera frugiperda (Lepidoptera, Noctuidae)

Roel¹,

Dourado

Matias

et al. 2010

Rev. Bras. entomol.

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ABSTRACT. The effect of sub-lethal doses of Azadirachta indica (Meliaceae) oil on the midgut of Spodoptera frugiperda (Lepidoptera, Noctuidae). The fall armyworm, Spodoptera frugiperda, is one of the major field pests for maize production. It is mainly controlled by means of synthetic, and more recently by resistant cultivar of maize expressing Bt toxins. The neem tree, Azadirachta indica, is a plant that can potentially control insects with the advantage of being food and environmental safe. The aim of this study was to assess the effect of neem oil on the development and survival of S. frugiperda caterpillars by assessing histological alterations caused on their midgut. Newly hatched caterpillars were submitted to three neem oil concentrations: 0.006; 0.05; 0.4%, which were added to their artificial diet. Ten 3 rd instar caterpillars, taken from each treatment, were submitted to histological analysis. The alimentary canals from the specimens were fixed in Baker for 12 hours, desiccated and diaphanized in alcohol/xylol (1:1) and xylol. After placing the samples in paraffin, they were sliced in 8 µm sections and stained with hematoxylineosin stain. The neem oil added to the diet of S. frugiperda caused total mortality at dose of 0.4% whilst still in the first instars, prolonged the larval and pupal stages, and reduced the pupal weight. Histo-physiological alterations such as degeneration of the epithelial lining of the midgut and in the peritrophic matrix were found at all concentrations of neem oil.KEYWORDS. Fall armyworm; histological analysis; insecticidal plants; neem. RESUMO. Efeito de doses subletais do óleo de Azadirachta indica (Meliaceae) no mesêntero de Spodoptera frugiperda (J. E. Smith) (Lepidoptera, Noctuidae). A lagarta-do-cartucho, Spodoptera frugiperda, é a mais importante praga da cultura do milho. Esta é usualmente, controlada por inseticidas sintéticos e mais atualmente por meio de variedades resistentes de milho com a toxina Bt. O nim Azadirachta indica é planta com potencial no controle de insetos, que possui as vantagens de segurança alimentar e ambiental. Objetivou-se com este estudo avaliar o efeito do óleo do nim no desenvolvimento e sobrevivência de lagartas de S. frugiperda em decorrência das alterações no mesêntero. As lagartas recém nascidas foram submetidas a três concentrações do óleo de nim: 0,006; 0,05; 0,4%, adicionadas à dieta artificial. Dez lagartas do 3º instar, retiradas de cada tratamento, foram submetidas à análise histológica. O canal alimentar dos indivíduos foi fixado em Baker por 12 horas, desidratado, diafanizado em álcool/xilol (1:1) e xilol. Após a inclusão em parafina, as amostras foram seccionadas em 8 µm e coradas pela técnica de hematoxilina-eosina. Observou-se que o óleo de A. indica adicionado à dieta de S. frugiperda causa: mortalidade total na dosagem 0,4% nos primeiros instares, aumento na duração do período larval e pupal, redução no peso de pupas. Alterações histofisiológicas, como degeneração do epitélio do revestimento do mesêntero, da matriz peritrófi...

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Section: Resultsmentioning

confidence: 94%

The effect of sub-lethal doses of Azadirachta indica (Meliaceae) oil on the midgut of Spodoptera frugiperda (Lepidoptera, Noctuidae)

Roel¹,

Dourado

Matias

et al. 2010

Rev. Bras. entomol.

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“…These enzymes are present in the insect guts of several orders and exo and endo types are probably expressed in agreement with the kind of diet used by the insect. In coleopterans, which feed on a diet rich in starch, the predominant endoglycosidases are the a-amylases (Ishimoto and Kitamura, 1989;Lemos et al, 1990;Grossi de Sa´and Chrispeels, 1997;Silva et al, 1999;Titarenko and Chrispeels, 2000;Cristofoletti et al, 2001). In Lepdopterans, which feed on leaves, exoglycosidase(s)-like b-glycosidases are predominantly present (Marana et al, 2000), and Hemipterans, which are plant feeders, use b-galactosidases to digest the carbohydrates present in their diet (Ferreira et al, 1998).…”

Section: Discussionmentioning

confidence: 99%

Digestive enzymes during development of Ceratitis capitata (Diptera:Tephritidae) and effects of SBTI on its digestive serine proteinase targets

Silva

Alcazar

Macedo

et al. 2006

Insect Biochemistry and Molecular Biology

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The digestive system of Ceratitis capitata was characterized during its larval development and in the insect stage. Disaccharidases against maltose and sucrose were more evident in the 2nd and 3rd day of larval development and in the adult stage, respectively. Glycosil-hydrolyses with higher specific a-galactosidasic and b-galactosidasic activities were detected in the 2nd and 3rd day of the larval stage, respectively. Specific proteolytic activities against azocasein showed an increase in the 4th and 5th day of the larval stage and in the adult stage. Specific hemoglobin activities were constant between 2nd and 6th day of the larval stage. The larvae used mainly serine proteinases, such as trypsin/chymotrypsin, and the adult insects only chymotrypsin-like enzymes in their digestive process. Two serine proteinases were separated from zymogram between the 4th and 5th day of larval development and in the adult stage. Effect of soybean trypsin inhibitor (SBTI, a serine proteinase inhibitor) on development of C. capitata was examined by bioassay. C. capitata was susceptible to SBTI which affected larval mass at ED 50 3.01%. r

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“…It is well known that the insect midgut is responsible for food digestion and nutrient absorption (Santos et al 1984, Billingsley & Lehane 1996, Cristofoletti et al 2001. The undigested material goes straight to the hindgut where the water absorption and feces formation and elimination occur.…”

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confidence: 99%

Morphological study of the hindgut in larvae of Anticarsia gemmatalis Hübner(Lepidoptera: Noctuidae)

Levy¹,

Falleiros²,

Moscardi³

et al. 2004

Neotrop. Entomol.

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Estudo Morfológico do Intestino Posterior em Larvas de Anticarsia gemmatalis Hübner (Lepidoptera: Noctuidae) RESUMO -A lagarta da soja (Anticarsia gemmatalis Hübner) tem grande interesse econômico, pois afeta significativamente a cultura da soja em todo o mundo. Este trabalho descreve a morfologia do intestino posterior de larvas de A. gemmatalis, com ênfase nos seus aspectos histológicos. O intestino posterior é constituído por regiões morfologicamente distintas, identificadas como piloro, íleo, cólon e reto. Independente da região, a parede do intestino posterior é constituída por fina cutícula, epitélio simples e camada muscular. A íntima cuticular apresenta espículas no anel intersticial posterior, entre o intestino médio e o posterior, e na região posterior do piloro. A musculatura do reto é formada por camada única de largas fibras circulares, diferindo das demais regiões do intestino posterior que apresentam duas camadas de fibras musculares. As extremidades distais dos túbulos de Malpighi atravessam as paredes do reto, constituindo o sistema criptonefridial característico de Lepidoptera. PALAVRAS-CHAVE: Tubo digestivo, histologia, lagarta da soja, inseto ABSTRACT -The velvetbean caterpillar (Anticarsia gemmatalis Hübner) has great economical interest as it affects the soybean crop worldwide. This work describes the morphology of the hindgut in A. gemmatalis larvae emphasizing their histological aspects. Distinct morphological regions, identified as pylorus, ileum, colon and rectum, constitute the hindgut of A. gemmatalis. A thin cuticular intima, a simple epithelium and muscular layer compose the hindgut wall, independent of the region. Microspines project from the cuticular intima in the posterior interstitial ring, between the midgut and the hindgut, and the posterior pyloric region. A single circular layer of large fibers, differing from the other hindgut regions that present two layers of muscular fibers, forms the rectal musculature. The distal ends of Malpighian tubules cross the rectum wall and constitute the cryptonephric excretory system typical in Lepidoptera larvae.

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Apocrine secretion of amylase and exocytosis of trypsin along the midgut of Tenebrio molitor larvae

Cited by 122 publications

References 34 publications

The effect of sub-lethal doses of Azadirachta indica (Meliaceae) oil on the midgut of Spodoptera frugiperda (Lepidoptera, Noctuidae)

The effect of sub-lethal doses of Azadirachta indica (Meliaceae) oil on the midgut of Spodoptera frugiperda (Lepidoptera, Noctuidae)

Digestive enzymes during development of Ceratitis capitata (Diptera:Tephritidae) and effects of SBTI on its digestive serine proteinase targets

Morphological study of the hindgut in larvae of Anticarsia gemmatalis Hübner(Lepidoptera: Noctuidae)

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