Apocarotenoids profiling in different Capsicum species

Zoccali, Mariosimone; Giuffrida, Daniele; Salafia, Fabio; Rigano, Francesca; Dugo, Paola; Casale, Monica

doi:10.1016/j.foodchem.2020.127595

Cited by 24 publications

(10 citation statements)

References 35 publications

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“…Although the literature reports several studies on the aroma and the content of capsaicinoids in chili peppers [ 17 ], to the best of our knowledge, capsaicinoids [ 18 , 19 ] and the volatile profile of chili-pepper-flavored extra virgin olive oil have not been investigated well [ 19 ]. For this reason, a study on the aroma profile of flavored EVOOs was carried out and compared to that of fresh peppers and unflavored olive oils.…”

Section: Resultsmentioning

confidence: 99%

Elucidation of Analytical–Compositional Fingerprinting of Three Different Species of Chili Pepper by Using Headspace Solid-Phase Microextraction Coupled with Gas Chromatography–Mass Spectrometry Analysis, and Sensory Profile Evaluation

Trovato

Vento

Creti³

et al. 2022

Molecules

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The aim of the present study was to determine the volatile compounds of three different species of chili peppers, using solid-phase microextraction (SPME) methods in combination with gas chromatography–mass spectrometry (GC-MS). The detection of marker aroma compounds could be used as a parameter to differentiate between species of chili peppers for their detection and traceability in chili pepper food. The sensorial contribution was also investigated to identify the predominant notes in each species and to evaluate how they can influence the overall aroma. Three different pepper species belonging to the Capsicum genus were analyzed: Chinense, Annuum, and Baccatum. A total of 269 volatile compounds were identified in these species of chili peppers. The Capsicum annum species were characterized by a high number of acids and ketones, while the Capsicum chinense and Capsicum baccatum were characterized by esters and aldehydes, respectively. The volatile profile of extra virgin olive oils (EVOOs) flavored with chili peppers was also investigated, and principal component analysis (PCA) and hierarchical cluster analysis (HCA) of the volatile profiles were demonstrated to be a powerful analytical strategy for building a model that highlights the potential of a volatile characterization approach for use in evaluating food traceability and authenticity.

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Section: Resultsmentioning

confidence: 99%

Elucidation of Analytical–Compositional Fingerprinting of Three Different Species of Chili Pepper by Using Headspace Solid-Phase Microextraction Coupled with Gas Chromatography–Mass Spectrometry Analysis, and Sensory Profile Evaluation

Trovato

Vento

Creti³

et al. 2022

Molecules

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“…Moreover, successive extractions were performed on the same sample, showing that three extraction steps were required for the total depletion of compounds. An identical hyphenated system was used for the analysis of carotenoids of tamarillo samples (Solanum betaceum) [44], for the comparison of 17 different Capsicum species [45] but also to identify apocarotenoids in microalgae strains [46].…”

Section: Application Of Sfc To the Analysis Of Carotenoidsmentioning

confidence: 99%

Supercritical fluid chromatography for the analysis of natural dyes: From carotenoids to flavonoids

Lesellier

West

2021

J of Separation Science

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Plant-derived natural dyes are used in a variety of formulated products, from food to cosmetics and pharmaceutics. In addition to their color, they also provide some bioactivity. While they are mostly analyzed with high-performance liquid chromatography, supercritical fluid chromatography was also employed for several dye families, mostly for carotenoids and chlorophylls, and more recently for anthraquinones and flavonoids. These supercritical fluid chromatography methods are described in this review. Because the dyes have different structures and structural variations (polarity, isomers, etc.), the best chromatographic system to achieve their separation is not always the same. Hydrophobic stationary phases are preferred for the most hydrophobic dyes (chlorophylls and carotenoids) while polar stationary phases are preferred for the polar dyes (anthraquinones and flavonoids). Regarding the mobile phase composition, chlorophylls and carotenoids are best eluted with moderate proportions of cosolvent in CO 2 (about 40%), while the most polar glycosylated flavonoids require higher proportions of co-solvent and acidic additives. Because dyes are colorful, ultraviolet-visible detection is often sufficient, while mass spectrometry offers additional structural information. Furthermore, fundamental information can also be gained through chromatographic analysis of dyes: either solubility in supercritical fluids, in view of their extraction, or retention behavior providing an understanding of stationary phase properties.

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“…Aliquots of stock solutions (100 μg/mL) of the external standard caffeine and the internal standards (IS) 13 C 3 -caffeine (1,3,7-tri-( 13 C-methyl)xanthine) and 32 S-6thiocaffeine were prepared weekly in Milli-Q water and stored at −20 • C. Working solutions of the external standard (10 and 1 μg/mL) and the IS (5 μg/mL) were prepared daily and used to spike blank saliva obtained from healthy individuals abstaining from caffeine-containing products.…”

Section: Standard Solutionsmentioning

confidence: 99%

“…Quantitative determinations by online SFE-SFC have been realized for blood [28], plasma [29], serum [30], urine [31], plant-based [32], and nonbiological matrices [33], largely made possible by the development of sensitive MS interfaces [34]. To the best of our knowledge, SFE-SFC-MS of saliva has not been reported [23,27,[35][36][37][38].…”

Section: Introductionmentioning

confidence: 99%

Supercritical fluid extraction–supercritical fluid chromatography of saliva: Single‐quadrupole mass spectrometry monitoring of caffeine for gastric emptying studies^†

Hofstetter

Schulig

Bethmann

et al. 2021

J of Separation Science

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Funding informationDeutsche Forschungsgemeinschaft; We acknowledge support by the German Saliva is an attractive sampling matrix for measuring various endogenous and exogeneous substances but requires sample treatment prior to chromatographic analysis. Exploiting supercritical CO 2 for both extraction and chromatography simplifies sample preparation, reduces organic solvent consumption, and minimizes exposure to potentially infectious samples, but has not yet been applied to oral fluid. Here, we demonstrate the feasibility and benefits of online supercritical fluid extraction coupled to supercritical fluid chromatography and singlequadrupole mass spectrometry for monitoring the model salivary tracer caffeine. A comparison of 13 C-and 32 S-labeled internal standards with external standard calibration confirmed the superiority of stable isotope-labeled caffeine over nonanalogous internal standards. As proof of concept, the validated method was applied to saliva from a magnetic resonance imaging study of gastric emptying. After administration of 35 mg caffeine via ice capsule, salivary levels correlated with magnetic resonance imaging data, corroborating caffeine's usefulness as tracer of gastric emptying (R 2 = 0.945). In contrast to off-line methods, online quantification required only minute amounts of organic solvents and a single manual operation prior to online bioanalysis of saliva, thus demonstrating

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Apocarotenoids profiling in different Capsicum species

Cited by 24 publications

References 35 publications

Elucidation of Analytical–Compositional Fingerprinting of Three Different Species of Chili Pepper by Using Headspace Solid-Phase Microextraction Coupled with Gas Chromatography–Mass Spectrometry Analysis, and Sensory Profile Evaluation

Elucidation of Analytical–Compositional Fingerprinting of Three Different Species of Chili Pepper by Using Headspace Solid-Phase Microextraction Coupled with Gas Chromatography–Mass Spectrometry Analysis, and Sensory Profile Evaluation

Supercritical fluid chromatography for the analysis of natural dyes: From carotenoids to flavonoids

Supercritical fluid extraction–supercritical fluid chromatography of saliva: Single‐quadrupole mass spectrometry monitoring of caffeine for gastric emptying studies^†

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Apocarotenoids profiling in different Capsicum species

Cited by 24 publications

References 35 publications

Elucidation of Analytical–Compositional Fingerprinting of Three Different Species of Chili Pepper by Using Headspace Solid-Phase Microextraction Coupled with Gas Chromatography–Mass Spectrometry Analysis, and Sensory Profile Evaluation

Elucidation of Analytical–Compositional Fingerprinting of Three Different Species of Chili Pepper by Using Headspace Solid-Phase Microextraction Coupled with Gas Chromatography–Mass Spectrometry Analysis, and Sensory Profile Evaluation

Supercritical fluid chromatography for the analysis of natural dyes: From carotenoids to flavonoids

Supercritical fluid extraction–supercritical fluid chromatography of saliva: Single‐quadrupole mass spectrometry monitoring of caffeine for gastric emptying studies†

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Product

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Supercritical fluid extraction–supercritical fluid chromatography of saliva: Single‐quadrupole mass spectrometry monitoring of caffeine for gastric emptying studies^†