Apo(a) phenotyping and long-term prognosis for coronary artery disease

Baños-González, Manuel Alfonso; Peña‐Duque, Marco Antonio; Anglès-Cano, Eduardo; Martínez-Ríos, Marco Antonio; Bahena, A.; Valente-Acosta, Benjamín; Cardoso-Saldaña, Guillermo; Angulo-Ortíz, J.; Peña-Dı́az, Aurora de la

doi:10.1016/j.clinbiochem.2010.01.013

Cited by 9 publications

(8 citation statements)

References 40 publications

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“…Based on previous studies [10,11,27], patients were followed up for about 7 years as a sufficient follow-up period. Information was also obtained for patients who no longer underwent medical care at the hospital.…”

Section: Methodsmentioning

confidence: 99%

Low-molecular-weight lipoprotein (a) and low relative lymphocyte concentration are significant and independent risk factors for coronary heart disease in patients with type 2 diabetes mellitus: Lp(a) phenotype, lymphocyte, and coronary heart disease

et al. 2013

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BackgroundThe aim of the present prospective study was to examine whether lipoprotein (a) [Lp(a)] phenotypes and/or low relative lymphocyte concentration (LRLC) are independently associated with coronary heart disease (CHD) in patients with type 2 diabetes mellitus (T2DM).MethodsSerum Lp(a) concentration, Lp(a) phenotypes, and RLC were analyzed in 214 subjects. Lp(a) phenotypes were classified into 7 subtypes according to sodium dodecyl sulfate-agarose gel electrophoresis by Western blotting. Subjects were assigned to the low-molecular-weight (LMW (number of KIV repeats: 11–22) ) and high-molecular-weight (HMW( number of KIV repeats: >22 )) Lp(a) groups according to Lp(a) phenotype and to the LRLC (RLC: <20.3%) and normal RLC (NRLC; RLC: ≥20.3%) groups according to RLC. A CHD event was defined as the occurrence of angina pectoris or myocardial infarction during the follow-up period.ResultsDuring the follow-up period, 30 cases of CHD events were verified. Neutrophil count showed no correlation with CHD, while relative neutrophil concentration and RLC showed positive and negative correlations, respectively, with CHD. The Cox proportional hazard model analysis revealed the following hazard ratios adjusted for LMW Lp(a), LRLC, and LMW Lp(a) + LRLC: (4.31; 95% confidence interval [CI], 1.99-9.32; P < 0.01, 3.621; 95% CI, 1.50-8.75; P < 0.05, and 7.15; 95% CI, 2.17-23.56; P < 0.01, respectively).ConclusionsOur results suggest that both LMW Lp(a) and LRLC are significant and independent risk factors for CHD and that the combination thereof more strongly predicts CHD in patients with T2DM.

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Section: Methodsmentioning

confidence: 99%

Low-molecular-weight lipoprotein (a) and low relative lymphocyte concentration are significant and independent risk factors for coronary heart disease in patients with type 2 diabetes mellitus: Lp(a) phenotype, lymphocyte, and coronary heart disease

et al. 2013

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“…These biothiols play important roles in many biological processes, including the maintenance of reduction-oxidation (redox) homeostasisagainst oxidative stress and the inactivation of toxic compounds [1,[7][8][9][10]. In addition, imbalances inbiothiol metabolism areclosely related to various pathologies such as vascular diseases [11,12], neurodegenerative diseases including Alzheimer's [13]and Parkinson's [14], cancers [15,16], and diabetes mellitus [17,18]. Therefore, the simultaneous determination of multiple biothiols in biological samples is important for clarifying the relationship between the in vivo redox status and these disease states.…”

Section: Introductionmentioning

confidence: 99%

Determination and characterization of total thiols in mouse serum samples using hydrophilic interaction liquid chromatography with fluorescence detection and mass spectrometry

Isokawa

Shimosawa

Funatsu

et al. 2016

Journal of Chromatography B

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Biothiols such as homocysteine, cysteine, and glutathione play many biologically important roles, especially in reduction-oxidation homeostasis and resistance to oxidative stress, and the measurement of their concentrations in model animal fluids is important in clarifying the pathology of thiol-related diseases. In this study, an analytical method for total biothiols in mouse serum using hydrophilic interaction liquid chromatography (HILIC) with fluorescence detection was developed. Mouse serum samples were derivatized with ammonium 7-fluoro-2,1,3-benzoxadiazole-4-sulfonate (SBD-F), after reduction by tris(2-carboxyethyl)phosphine. Five biothiols (homocysteine, cysteine, cysteinylglycine, glutathione, and γ-glutamylcysteine) in the mouse sera were separated within 16 min on an amide-type HILIC column. The method possessed good linearity, good reproducibility with an intra-day variance of less than 3%, and low detection limits of 0.2-4 nM. Concentrations of homocysteine, cysteine, cysteinylglycine, glutathione, and γ-glutamylcysteine in the mouse serum samples were calculated as 6.7 ± 0.3, 227.7 ± 16.9, 1.2 ± 0.4, 77.5 ± 29.2, and 8.2 ± 0.9 μM, respectively (mean ± S.D., n = 4). Furthermore, HILIC-negative electrospray ionization-mass spectrometry (MS) analysis using a high-resolution mass spectrometer was conducted to determine the exact masses of two unknown peaks, which were found in the mouse serum samples with high signal intensity and were not detected in human plasma samples. The exact masses of the unknown compounds were determined as 1184.519 and 800.281 (as SBD-derivatized negative ions), which possessed a product ion common to SBD-thiols (m/z 230.954, as [SBD-SH](-)) upon tandem MS spectrometric analysis.

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“…Substantial evidence supports the notion that enhanced formation of reactive oxygen and nitrogen species (ROS/RNS) leads to oxidative stress and participates in the pathogenesis of atherosclerosis, peripheral vascular and coronary heart diseases [2]. Oxidative modification of lipids and molecules is considered to be a key event in the pathogenesis of atherosclerosis and atherothrombotic cardiovascular disease [3,4], promoting conversion of low‐density lipoproteins (LDL) [2,5] and apolipoprotein(a) [apo(a)] into more atherogenic forms [6] and adversely affecting levels of asymmetric dimethylarginine (ADMA) [7] and fetuin A [8].…”

Section: Introductionmentioning

confidence: 99%

Age‐related modification of antioxidant enzyme activities in relation to cardiovascular risk factors

Fabian

Bogner

Elmadfa

2011

Eur J Clin Investigation

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Apo(a) phenotyping and long-term prognosis for coronary artery disease

Abstract: Small-sized apo(a) isoforms are an independent risk factor for MACCE in patients with coronary artery disease in follow-up. Lp(a) plasma concentration and apo(a) fibrin-binding were associated, although not significant.

Cited by 9 publications

References 40 publications

Low-molecular-weight lipoprotein (a) and low relative lymphocyte concentration are significant and independent risk factors for coronary heart disease in patients with type 2 diabetes mellitus: Lp(a) phenotype, lymphocyte, and coronary heart disease

Low-molecular-weight lipoprotein (a) and low relative lymphocyte concentration are significant and independent risk factors for coronary heart disease in patients with type 2 diabetes mellitus: Lp(a) phenotype, lymphocyte, and coronary heart disease

Determination and characterization of total thiols in mouse serum samples using hydrophilic interaction liquid chromatography with fluorescence detection and mass spectrometry

Age‐related modification of antioxidant enzyme activities in relation to cardiovascular risk factors

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