Aplysia neurons express a gene encoding multiple FMRFamide neuropeptides

Schaefer, Mark; Picciotto, Marina R.; Kreiner, Thane; Kaldany, R R; Taussig, Ronald; Scheller, Richard H.

doi:10.1016/s0092-8674(85)80019-2

Cited by 182 publications

(86 citation statements)

References 43 publications

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“…RHA3, RHAI, and RHA2 contained three, four, and five copies, respectively. Such a repeated arrangement has been also observed in a-factor precursors of several Silcchaiaony(vC'es species (9) and in the neuropeptide FMRFamide (44). The generation of these multiple repeats could be explained by an unequal crossing-over mechanism (49).…”

mentioning

confidence: 67%

Multiple genes coding for precursors of rhodotorucine A, a farnesyl peptide mating pheromone of the basidiomycetous yeast Rhodosporidium toruloides.

Akada¹,

Minomi²,

Jiao³

et al. 1989

Mol. Cell. Biol.

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mentioning

confidence: 67%

Multiple genes coding for precursors of rhodotorucine A, a farnesyl peptide mating pheromone of the basidiomycetous yeast Rhodosporidium toruloides.

Akada¹,

Minomi²,

Jiao³

et al. 1989

Mol. Cell. Biol.

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“…The cDNA clones encoding Aplysia actin (pLT2.5) and Aplysia sensorin A (SCR2; Brunet et al, 1991) were kindly made available by Dr. E. R. Kandel, Columbia University. The cDNA clone encoding ApZysia FMRFamide (FMRF-1; Schaefer-et al, 1985) was kindly made available by Dr. R. Scheller, Stanford University. The plasmid containing the cDNA inserts were digested with EcoRl and One hour after the crush, ganglia were removed and the nerves were cut 4 cm from the pedal ganglion.…”

Section: Methodsmentioning

confidence: 99%

Recovery of tail-elicited siphon-withdrawal reflex following unilateral axonal injury is associated with ipsi- and contralateral changes in gene expression in Aplysia californica

et al. 1995

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Behavioral, cellular and molecular changes were examined following axonal injury in the marine mollusc Aplysia californica. Unilateral nerve injury was performed by crushing the pleural-pedal connective and the peripheral pedal nerves innervating one side of the posterior body wall and the tail. The injury procedure severs the axons of the pleural sensory neurons resulting in the blockade of the tail-elicited siphon- withdrawal reflex. Partial reflex recovery is observed within 3 d and reaches 50% of the pretest value by six weeks postinjury. Retrograde staining of injured nerves combined with electrophysiological recordings from siphon motor neurons show that axons can regenerate through the crushed site and reconnect with the tail by three weeks postinjury. Moreover, the behavioral and electrophysiological measurements suggest that the contralateral sensory neurons may contribute to the early recovery of the siphon-withdrawal reflex. The levels of mRNAs coding for actin and calreticulin are elevated while the mRNAs coding for intermediate filament protein, sensorin A, FMRFamide are reduced in the ipsilateral pleural ganglia as detected by Northern blots. In the contralateral pleural ganglia, the levels of mRNAs coding for actin, sensorin A and FMRFamide are elevated. These molecular changes in both the ipsi- and contralateral sides are consistent with the hypothesis that both sides are participating in the behavioral recovery following unilateral axonal injury.

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“…FMRFamide immunoreactivity has been found in different animal phyla (Boer, Schot, Veenstra & Reichelt, 1980) and recently in Aplysia (Weiss, Goldberg, Chohan, Stell, Drummond & Lukowiak, 1984). Identified neurones of Aplysia have also been shown to express a gene encoding multiple copies of FMRFamide (Schaefer, Picciotto, Kreiner, Kaldany, Taussig & Scheller, 1985).…”

Section: Introductionmentioning

confidence: 99%

Modulation of potassium conductances by an endogenous neuropeptide in neurones of Aplysia californica.

Březina¹,

Eckert²,

Erxleben³

1987

The Journal of Physiology

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SUMMARY1. Macroscopic and single-channel currents were recorded from voltage-clamped neurones in the abdominal and pleural ganglia of Aplysia californica in order to investigate conductance changes elicited by application of the endogenous peptide FMRFamide (Phe-Met-Arg-Phe-NH2) and related neuropeptides to the cell surface.2. The Ca-dependent K current, IK(Ca) when elicited at a constant voltage by intracellular injection of Ca2+, was insensitive to FMRFamide or its derivative YGG-FMRFamide (Tyr-Gly-Gly-Phe-Met-Arg-Phe-NH2). Unclamped cells responded with a corresponding hyperpolarization. These responses reversed at about -75 mV. Ion substitution indicated that the current is carried by K+. 4. FMRFamide and YGG-FMRFamide were equally effective in activating the outward current, whereas FMRF, met-enkephalin and leu-enkephalin were ineffective.5. At voltages negative to -30 mV and, in the absence of extracellular Ca2+, also at more positive potentials, the FMRFamide-sensitive current showed no voltage dependence beyond that predicted from constant-field considerations.6. The response to FMRFamide was relatively insensitive to extracellular tetraethylammonium (TEA, KD -75 mm) and 4-aminopyridine (4-AP, KD 6 mM). It was suppressed in Ba-containing solutions, but was unaffected by injection of the Ca chelating agent EGTA. The response was blocked by serotonin and other agents known to elevate intracellular adenosine 3',5'-phosphate (cyclic AMP) levels, and by direct injection of cyclic AMP into the cell. 7. In its pharmacological properties and lack of voltage dependence, the FMRFamide-activated current resembles the 'S' current, IK(S)' a K current suppressed by application of serotonin in Aplysia neurones.8. The similarity between the FMRFamide-sensitive current and the 'S' current was confirmed in cell-attached patch-clamp studies, in which activity of 'S' channels was found to be reduced by serotonin, and enhanced by FMRFamide.* Address for reprint requests.

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Aplysia neurons express a gene encoding multiple FMRFamide neuropeptides

Cited by 182 publications

References 43 publications

Multiple genes coding for precursors of rhodotorucine A, a farnesyl peptide mating pheromone of the basidiomycetous yeast Rhodosporidium toruloides.

Multiple genes coding for precursors of rhodotorucine A, a farnesyl peptide mating pheromone of the basidiomycetous yeast Rhodosporidium toruloides.

Recovery of tail-elicited siphon-withdrawal reflex following unilateral axonal injury is associated with ipsi- and contralateral changes in gene expression in Aplysia californica

Modulation of potassium conductances by an endogenous neuropeptide in neurones of Aplysia californica.

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