Aplicações da curva ROC em estudos clínicos e experimentais

Polo, Tatiana Cristina Figueira; Miot, Hélio Amante

doi:10.1590/1677-5449.200186

Cited by 59 publications

(65 citation statements)

References 30 publications

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“…ROC analysis depicted the comparisons of cut-off values with respect to sensitivity and specificity of K. Similarly, the AUC explained the overall performance of diagnostic test. AUC = 0.5 is not good and non-informative for the diagnostic test, AUC = between 0.5 and 0.7 depicts the less accurate value for the diagnostic test, AUC = between 0.7 and 0.9 indicates the moderately accurate, AUC = between 0.9 and 1 indicates the highly accurate value for the diagnostic test while AUC = 1.000 indicates the perfect test, and the diagnostic test is perfect for the diagnosis of positive and negative sera [ 45 ]. Subsequently, AUC = 1.000 of K indicated that this test was perfect test for the diagnosis of positive and negative sera of toxoplasmosis.…”

Section: Resultsmentioning

confidence: 99%

“…The ROC is used to find out the cut-off value of ELISA for the detection of positive and negative sera for anti- T. gondii antibodies. It is also used to determine the efficacy of diagnostic kits for detection of antibodies [ 44 , 45 ]. In the study of K development, the cut-off value was 0.398, AUC for K was 1.000, and it was highly significant ( p < 0.001).…”

Section: Discussionmentioning

confidence: 99%

“…The AUC explains the overall output of diagnostic test. AUC = 0.5 is not good for the diagnostic test, AUC = between 0.5 and 0.7 depicts the less accurate value, AUC = between 0.7 and 0.9 is moderately accurate, AUC = between 0.9 and 1 depicts the highly accurate value while AUC = 1.000 indicates the perfect test [ 45 ]. Mangili et al (2009) used ROC curve to find the AUC for the evaluation and performance of in-house ELISA for diagnosis of toxoplasmosis from serum.…”

Section: Discussionmentioning

confidence: 99%

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Development of Human Toxo IgG ELISA Kit, and False-Positivity of Latex Agglutination Test for the Diagnosis of Toxoplasmosis

et al. 2021

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Toxoplasma gondii is an intracellular zoonotic parasite that causes infection in a wide range of warm-blooded animals and humans. The main aim of this studywas to assess the diagnostic value of the recombinant SAG1 antigen (rSAG1) for T. gondii-IgG screening through the Human Toxo IgG ELISA Kit (K). The rSAG1 was expressed in E. coli (DE3), and it was purified through metal-affinity chromatography. The rSAG1 was confirmed by immunoblotting, and it had a band on 35 kDa.Total of 400 human sera were tested by LAT and K. One hundred and twenty-two (30.5%) sera were found positive by LAT and eighty-nine (22.25%) sera were found positive by K.Out of 400 samples, 80 were selected to evaluate the performance of K through commercial Toxoplasma gondii IgG ELISA Kit (C). Out of 80 human sera, 55 (68.75%) were found positive, 25 (31.25%) were found negative by K and C, respectively. The cut-off value for K was 0.398 and it was calculated through the receiver operator characteristic curve. The ELISA plates were coated at optimized concentration of rSAG1 = 0.125 µg/mL, and the test was performed by diluting the sera at 1:50. The sensitivity and specificity of K were observed to be 98.5% and 100%, respectively. The six sera (K-L+were found positive through LAT and these human sera were later evaluated by Western blot analysis. These sera did not produce a band equivalent to 35 kDa on WB analysis thus, LAT produced false-positive results

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Development of Human Toxo IgG ELISA Kit, and False-Positivity of Latex Agglutination Test for the Diagnosis of Toxoplasmosis

et al. 2021

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“…Para recursos de mensuração quantitativa, a análise da curva ROC representa a ferramenta mais adequada para o estabelecimento desses parâmetros. 20…”

Section: Materiais E Métodounclassified

Validação de um instrumento simples e de aplicação rápida para rastrear incapacidade em pacientes com hanseníase

Pinho

Freitas

Fontes

2022

Rev Bras Ortop (Sao Paulo)

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Resumo Objetivo Avaliar um instrumento diagnóstico simples e rápido a ser utilizado por qualquer profissional da saúde para rastrear a incapacidade apresentada por pacientes com hanseníase. Método Estudo de validação de teste de diagnóstico realizado em uma amostra de 156 pacientes com hanseníase para rastrear incapacidade funcional, por meio do questionário abreviado disabilities of arm, shoulder, and hand (QuickDASH). A avaliação neurológica simplificada proposta pela Organização Mundial da Saúde foi utilizada como referência. Construiu-se a curva de características operacionais do receptor (ROC) para determinação do ponto de corte do QuickDASH que melhor discriminou pacientes com incapacidade funcional provocada pela hanseníase. Resultados Foram identificados 86 (55,5%) pacientes com incapacidade funcional pela avaliação neurológica simplificada. O desempenho do QuickDASH mostrou que, em ponto de corte de 30 pontos, a sensibilidade e a especificidade foram de 72,1% e 68,1% (acurácia de 70,3%), respectivamente, para identificar incapacidade funcional, com um valor preditivo positivo de 73,8%. Conclusão O instrumento QuickDASH mostrou boa acurácia para rastrear incapacidade funcional no paciente com hanseníase, podendo ser útil na prática clínica da atenção básica e ambulatorial geral, com o objetivo de identificar pacientes que necessitam de referência especializada para sua prevenção e tratamento.

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“…Contudo, ao passo que se emprega a estatística para concluir quanto à diferença entre amostras, a maior variabilidade das medidas e a modesta diferença entre grupos comprometem o poder analítico (erro tipo II). Esse detalhe exige uma cuidadosa interpretação do p-valor (significância estatística) e da dimensão do efeito na inferência resultante de estudos de comparação entre grupos, apesar desses conceitos se aplicarem também a análises de correlação, concordância, sobrevivência, testes diagnósticos, entre outros [1][2][3][4][5] .…”

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