Aplicación de la espectrometría de masas en micobacterias

Alcaide, Fernando; Palop-Borrás, Begoña; Domingo, Diego; Tudó, Griselda

doi:10.1016/s0213-005x(16)30188-4

Cited by 6 publications

(1 citation statement)

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“…Ueki, et al, describieron en su trabajo que el 97,8 % de los casos de micobacteriosis diseminada se debía al complejo M. avium; en los pacientes con infección concomitante con el HIV, esta especie también predominó (80,2 %)(16).En los últimos años, el diagnóstico de las micobacterias no tuberculosas como agentes etiológicos se ha incrementado con el desarrollo y la introducción de métodos más sensibles en la rutina de los laboratorios, así como con el reconocimiento de nuevas especies asociadas con enfermedades(28)(29)(30)(31). En Colombia, Castro, et al, identificaron las siguientes especies de micobacterias utilizando la técnica de análisis de patrones de restricción: M. fortuitum, M. abscessus, M. intracellulare, M. gordonae, M. avium, M. chelonae, M. scrofulaceum, M. szulgai, M. terrae y M. malmoense; al comparar estos datos con los nuestros, se observó que el uso de la metodología Genotype CM/AS™ ha permitido detectar especies como M. kansasii, M. mucogenicum y M. simiae (10,14).Una limitación de este estudio la constituyó el diligenciamiento del formato único de vigilancia de las micobacterias, ya que la información no es completa en cuanto a la presencia de otros factores predisponentes asociados, así que, en los casos en que no se registró la información completa, no fue posible asegurar que estos no existieran, lo cual constituye un sesgo.…”

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Micobacteriosis identificadas en el Laboratorio Nacional de Referencia de Colombia entre 2012 y 2016

2018

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Introduction: In recent years, there has been an increase in the prevalence of mycobacterioses caused by non-tuberculous mycobacteria, which are considered as emerging pathogens. Their presence depends on several factors such as the clinical history, the health status of the affected person, and the presence of these microorganisms in the water, the soil, and the animals, among others. Objective: To describe the mycobacteria and the etiological agent identified in isolates received at the Laboratorio Nacional de Referencia de Micobacterias of the Instituto Nacional de Salud between 2012 and 2016. Materials and methods: We conducted a retrospective analysis of samples from 273 patients with mycobacterioses. We analyzed the following variables: mycobacteriosis type, etiological agent, and associated predisposing factors. Results: 57.1% of the cases presented pulmonary mycobacteriosis; 26%, cutaneous; 10.6%, disseminated, and 2.6%, lymphatic. We found the Mycobacterium avium complex more frequently in pulmonary mycobacteriosis, while M. abscessus was more frequent in the extrapulmonary types of the disease. Patients with pulmonary mycobacteriosis had a history of tuberculosis more frequently than those with extrapulmonary forms. Conclusion: These findings highlight the importance of the differential diagnosis between M. tuberculosis complex species and non-tuberculous mycobacteria since the latter are genetically resistant to conventional antituberculosis drugs.

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Micobacteriosis identificadas en el Laboratorio Nacional de Referencia de Colombia entre 2012 y 2016

2018

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Identification of Mycobacterium Species by MALDI-TOF Mass Spectrometry

Neuschlová

Vladarova

Kompaníková

et al. 2017

Advances in Experimental Medicine and Biology

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Matrix-assisted laser desorption ionization - time-of-flight (MALDI-TOF) mass spectrometry enables to identify microorganisms by comparison of the protein content with reference spectra in the database. The aim of this study was to evaluate the efficacy of phenotypic identification of mycobacteria by MALDI-TOF mass spectrometry in laboratory practice. Seventy five isolates of mycobacteria were identified by molecular and phenotypic method, and the results were compared by MALDI-TOF. For MALDI-TOF, material was processed according to the Bruker Daltonics protocol and Mycobacterial Library database version 2.0, with 313 reference mycobacteria spectra. All except one of the 72 isolates agreed with regard to the species and genus by both methods. Forty three isolates were identified as the M. tuberculosis complex by MALDI-TOF. Thirty one isolates of nontuberculous mycobacteria were consistently identified by both methods to the species level. We conclude that MALDI-TOF mass spectrometry is an accurate method of bacterial identification. Simplicity, speed, and economic availability of the method makes it suitable for mycobacteria identification in a routine laboratory.

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Diagnóstico microbiológico actual de la tuberculosis

Alcaide

2017

Enfermedades Infecciosas y Microbiología Clínica

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Aplicación de la espectrometría de masas en micobacterias

Cited by 6 publications

References 0 publications

Micobacteriosis identificadas en el Laboratorio Nacional de Referencia de Colombia entre 2012 y 2016

Micobacteriosis identificadas en el Laboratorio Nacional de Referencia de Colombia entre 2012 y 2016

Identification of Mycobacterium Species by MALDI-TOF Mass Spectrometry

Diagnóstico microbiológico actual de la tuberculosis

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