In recent years, escalating chemical fertilizer costs have imperiled the economic feasibility of coffee cultivation in Peru, resulting in reduced yields across plantations. This study aimed to assess biofertilizers' effect on the growth and biomass of three coffee varieties. Employing a complete factorial design (A×B), Factor A encompassed three coffee varieties: Castillo, Catuaí, and Obata, while Factor B comprised five treatments: edaphic fertilization combined with humic acids (FEAH), edaphic fertilization combined with Trichoderma (FET), edaphic fertilization combined with mycorrhizae (FEM), edaphic fertilization (FE), and a no-fertilization control group (SF), totaling 15 treatments. This study included biometric measurements of height, leaf count, stem diameter, total fresh biomass, and total dry biomass. Notably, the Castillo variety exhibited promising outcomes, boasting a plant height of 15.8 cm, stem diameter of 4.30 mm, leaf count of 12.14, fresh weight of 10.82 g per plant, and total dry weight of 2.26 g per plant.Comparative assessment of technological interventions revealed superior biometric results and enhanced biomass with FEAH and FEM compared with FET, FE, and SF. Biofertilizers and humic acids presented favorable impacts. This study contributes to addressing the challenges faced by coffee growing in Peru and provides information for sustainable coffee growth practices that promote economic and environmental well-being.