Apigenin produced by maize flavone synthase <scp>I</scp> and <scp>II</scp> protects plants against <scp>UV‐B</scp>‐induced damage

Righini, Silvana; Rodrı́guez, Eduardo; Berosich, Carla; Grotewold, Erich; Casati, Paula; Ferreyra, Marı́a Lorena Falcone

doi:10.1111/pce.13428

Cited by 67 publications

(42 citation statements)

References 95 publications

(161 reference statements)

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“…Previous studies have reported that maysin and rhamnosylisoorientin, two UV-absorbing flavones, accumulate in maize leaves of high-altitude lines in response to UV-B radiation [11]. Overexpression of either the maize FNS enzyme (ZmFNSI and ZmFNSII) in Arabidopsis was shown to decrease UV-B induced damage [12]. Additionally, accumulation of apigenin 5-O-glucoside in rice enhanced its tolerance to UV radiation [35].…”

Section: Discussionmentioning

confidence: 97%

“…The continuous thinning of the atmospheric ozone layer in recent years has caused an increase in damage to plants by solar UV radiation. Several studies have shown that the content of flavones is higher in the leaves of plants growing at high altitudes than in those of plants growing at low altitudes, demonstrating a correlation between flavones and plant tolerance to UV stress [11,12]. Other significant properties of certain flavones are their nutritional value and bioactivities in humans, including antioxidant and putative anticancer activity [1,13].…”

Section: Introductionmentioning

confidence: 99%

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Flavones Produced by Mulberry Flavone Synthase Type I Constitute a Defense Line against the Ultraviolet-B Stress

Yang

et al. 2020

Plants

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Flavones, one of the largest classes of flavonoids in plants, have a variety of bioactivities and participate in the resistance response of plants to biotic and abiotic stresses. However, flavone synthase (FNS), the key enzyme for flavone biosynthesis, has not yet been characterized in mulberry. In this study, we report that the leaves of certain mulberry cultivars, namely BJ7, PS2, and G14, are rich in flavones. We identified a Fe2+/2-oxoglutarate-dependent dioxygenase from Morus notabilis (MnFNSI) that shows the typical enzymatic activity of a FNSI-type enzyme, and directly converts eriodictyol and naringenin into their corresponding flavones. Overexpression of MnFNSI in tobacco increased the flavones contents in leaves and enhanced the tolerance of tobacco to ultraviolet-B (UV-B) stress. We found that mulberry cultivars with higher flavones contents exhibit less UV-B induced damage after a UV-B treatment. Accordingly, our findings demonstrate that MnFNSI, a FNSI-type enzyme, is involved in the biosynthesis of flavones, which provide protection against UV-B radiation. These results lay the foundation for obtaining mulberry germplasm resources that are more tolerant to UV-B stress and richer in their nutritional value.

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Section: Discussionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Flavones Produced by Mulberry Flavone Synthase Type I Constitute a Defense Line against the Ultraviolet-B Stress

Yang

et al. 2020

Plants

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“…Various flavonoids and pigment-containing extracts have been shown to protect DNA from damage. For example, apigenin protects plants against damage induced by ultraviolet (UV)-B radiation [5]; Chinese bayberry extract protects against oxidative DNA damage [6]; grape-seed extract prevents oxidation-induced DNA damage [7]; and cyanidin-3-glucoside, along with its aglycon, protect against calf thymus DNA (ctDNA) damage [8].…”

Section: Introductionmentioning

confidence: 99%

DNA Binding Characteristics and Protective Effects of Yellow Pigment from Freshly Cut Yam (Dioscorea opposita)

Zhao

Zhao²,

Ma³

et al. 2020

Molecules

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Yam yellow pigments (YP) are natural pigments formed during the storage of freshly cut yam (Dioscorea opposita) under certain conditions. The interaction of YP with calf thymus DNA (ctDNA) and its protective effect against DNA oxidative damage were investigated using multiple spectroscopic techniques, competitive binding experiments, viscosity measurements, and gel electrophoresis. Results showed that YP participated in intercalative binding with ctDNA. YP exhibited a protective effect against hydroxyl-induced DNA damage, which was attributed to the high hydroxyl radical scavenging activity of YP. Our findings improve our understanding of the mechanism of interaction between YP and ctDNA, and provide a theoretical basis for the application of YP in the food and drug industry.

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“…There are also other genes like FNS and IFS could catalyze the flavone and isoflavone biosynthesis [14,15]. Furthermore, the transcriptional factors regulating the biosynthesis were identified in many species.…”

Section: Introductionmentioning

confidence: 99%

Long-read sequencing of Chrysanthemum morifolium cv. ‘Hangju’ transcriptome reveals flavonoid biosynthesis and regulation

Wang

Yang

et al. 2019

Preprint

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Background : The capitulum of Chrysanthemum morifolium cv. ‘Hangju’ has been widely used in China for antioxidant and anti-inflammatory. Flavonoids as one of the bioactive components in C . morifolium have a poor understanding in their biosynthesis and regulation. Nowadays, transcriptome sequencing as an effective method was used in capturing the transcripts information. So, single-molecule real-time (SMRT) sequencing was performed to obtain the full length of genes involved in flavonoid biosynthesis and regulation in C . morifolium . Results : The high-quality RNA was extracted from the capitulum of C . morifolium at different development stages, and it was constructed into two libraries (0-5 kb and 4.5-10 kb) for sequencing. Finally, 125,532 non-redundant isoforms with mean length of 2,009 bp were captured. Of which, 2,083 transcripts were annotated in the pathway related to the flavonoid biosynthesis and 56 isoforms were annotated as CHS , CHI , F3H , F3’H , FNS Ⅱ , FLS , DFR and ANS genes. Based on the gene expression level at different stages, we predicted the major genes involved in the flavonoid biosynthesis. And we found two candidate MYB factors (CmMYBF1 and CmMYBF2) activating the flavonol biosynthesis by phylogenetic analysis. Conclusions : Based on the full-length transcriptome data and further quantitative analysis, the major genes involved in flavonoid biosynthesis and regulation in C . morifolium were predicted in our study. The results provide a valuable theoretical basis for introduction and cultivation of C. morifolium cv. ‘Hangju’.

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Apigenin produced by maize flavone synthase I and II protects plants against UV‐B‐induced damage

Cited by 67 publications

References 95 publications

Flavones Produced by Mulberry Flavone Synthase Type I Constitute a Defense Line against the Ultraviolet-B Stress

Flavones Produced by Mulberry Flavone Synthase Type I Constitute a Defense Line against the Ultraviolet-B Stress

DNA Binding Characteristics and Protective Effects of Yellow Pigment from Freshly Cut Yam (Dioscorea opposita)

Long-read sequencing of Chrysanthemum morifolium cv. ‘Hangju’ transcriptome reveals flavonoid biosynthesis and regulation

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