Apical CD36 immunolocalization in human and porcine taste buds from circumvallate and foliate papillae

Simons, Peter J.; Kummer, J. Alain; Luiken, Joost J. F. P.; Boon, Louis

doi:10.1016/j.acthis.2010.08.006

Cited by 120 publications

(76 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The current study provides the fi rst experimental evidence to demonstrate that CD36 is involved in fat gustatory perception in humans as observed previously in rodents ( 25,27,38 ). Although we did not measure CD36 expression in tongue tissue, CD36 has been identifi ed in human taste bud cells ( 28 ).…”

Section: Infl Uence Of Orlistat On Oleic Acid and Triolein Detection mentioning

confidence: 85%

The fatty acid translocase gene CD36 and lingual lipase influence oral sensitivity to fat in obese subjects

Pepino

Love‐Gregory

Klein

et al. 2012

Journal of Lipid Research

244

226

View full text Add to dashboard Cite

Obesity is caused by ingesting more energy than is expended over a long period of time. Dietary fat is the most energy-dense macronutrient, and its overconsumption has been linked to obesity ( 1-4 ). Obese people prefer foods with higher fat content ( 5 ), crave high-fat foods more frequently ( 6, 7 ), and consume more fat than lean individuals ( 8 ).Traditionally, perception of fat in the oral cavity is thought to rely almost entirely on textural and aromatic cues activating the somatosensory and olfactory systems. However, there is now increasing evidence to support an important role of the gustatory system in fat perception ( 9-20 ) as well as in intestinal lipid metabolism ( 10, 21 ). Oral and gastrointestinal fat sensory sensitivity appear to be associated ( 16 ) and there is similarity in the chemosensory reception events and their signaling transduction pathways in the tongue and gastrointestinal tract ( 21 ). An important requirement for the involvement of a gustatory component in dietary lipid detection is the hydrolysis of triacylglycerols (TGs) to release free FAs, the signaling stimulus, as was demonstrated through the use of the lipase inhibitor orlistat ( 22 ). In rodents, lingual lipase is essential for the gustatory perception of dietary fats ( 22 ) and the addition of orlistat to fat emulsions diminishes the rat's preference for TG, but not FA ( 22 ). Although it is not known whether lingual lipase is important for oral fat perception in humans, data from a recent study suggests that lingual lipase lipolytic activity can produce FA within the concentration range required to activate oral sensors ( 18 ).Several putative fat taste receptor classes have been identifi ed in rodents ( 12,23,24 ), including the glycoprotein CD36 ( 25 ). The presence of CD36, a scavenger receptor that mediates uptake and traffi cking of lipids in diverse cell types ( 26 ), has been documented in the gustatory papillae of rodents ( 25, 27 ), pigs, and humans ( 28 ). In rodents, the interaction between CD36 and FA results in signaling events that depend on an intact neuronal gustatory pathway ( 15,29 ). CD36 gene knockout impedes fat detection in mice without affecting sweet or bitter perception Abstract The precise orosensory inputs engaged for dietary lipids detection in humans are unknown. We evaluated whether a common single nucleotide polymorphism (rs1761667) in the CD36 gene that reduces CD36 expression and the addition of orlistat, a lipase inhibitor, to reduce FA release from triacylglycerols (TGs), the main component of dietary fats, would attenuate fat orosensory sensitivity in humans. Twenty-one obese subjects with different rs1761667 genotypes (6 AA, 7 AG, and 8 GG) were studied on two occasions in which oleic acid and triolein orosensory detection thresholds were measured using emulsions prepared with and without orlistat. Subjects homozygous for the G-allele had 8-fold lower oral detection thresholds for oleic acid and triolein than subjects homozygous for the A allele, which associates with lower CD36 expr...

show abstract

Section: Infl Uence Of Orlistat On Oleic Acid and Triolein Detection mentioning

confidence: 85%

The fatty acid translocase gene CD36 and lingual lipase influence oral sensitivity to fat in obese subjects

Pepino

Love‐Gregory

Klein

et al. 2012

Journal of Lipid Research

244

226

View full text Add to dashboard Cite

show abstract

“…Although evidence is also mounting in humans (14)(15)(16)(17)(18)(19), much less is known about the orosensory perception of fat constituents relative to rodent models. However, it appears conceivable that the same group of candidate receptors shown in rodents or a subset thereof, such as CD36 ( 20 ) and GPR120 ( 18 ), may contribute to human fat perception as well.…”

Section: Sensory Analysesmentioning

confidence: 99%

The role of lipolysis in human orosensory fat perception

Voigt

Stein

Galindo

et al. 2014

Journal of Lipid Research

View full text Add to dashboard Cite

This article is available online at http://www.jlr.org the fi ve taste qualities, sweet, umami, salty, sour, and bitter ( 2 ). In the past, the existence of additional taste modalities such as fatty ( 3 ), metallic ( 4 ), or a taste for water ( 5 ) has been speculated. In recent years, particular attention has been paid to the potential existence of fat taste and its putative status compared with the other well-accepted taste qualities . Whereas the textural, olfactory, and postingestive recognition of fat constituents have been considered the dominant cues for fat perception in the past, several recent studies, performed mostly in rodents but also in humans, have pointed to a gustatory component in fat perception [for a recent review see ( 6 )]. The use of anosmic ( 7 ) or esophagostomized ( 8 ) rat models in combination with texture-masking buffer compositions indicated an orosensory detection mechanism for long-chain fatty acids, in particular. Moreover, a number of candidate receptors for the oral detection of lipophilic molecules were identifi ed by several independent research groups. These putative fat sensors include potassium channels ( 3 ) and scavenger receptor/fatty acid transporter CD36/FAT ( 9, 10 ), as well as G protein-coupled receptors (GPRs) such as GPR40 ( 11 ) and GPR120 ( 11-13 ). For some of the candidate receptors, knockout mouse models were analyzed and their contribution to orosensory fat perception confi rmed. Mice with a genetic ablation of CD36 exhibit no preference for the polyunsaturated long-chain fatty acid, linoleic acid, in two-bottle preference tests compared with wild-type mice. Moreover, a reduced cephalic phase response upon oral stimulation with oleic, linoleic, and linolenic acid, but not to the saturated long-chain fatty acid, stearic acid, or the medium-chain fatty acid, caprylic acid, suggests pronounced selectivity for particular lipophilic stimuli ( 10 ). Similarly, GPR40 as well as GPR120 knockout mice both show a loss of preference for linoleic acid, and The perception of taste elicited by countless chemicals present in food plays an important role for the survival of organisms. The gustatory system monitors not only the caloric content and the appropriate electrolyte supply, but also the presence of putatively harmful substances in food items ( 1 ). Detection of food constituents within the oral cavity is achieved by taste receptor molecules expressed by sensory cells specifi cally devoted to the detection of one of

show abstract

“…En effet, elle lie les acides gras à longue chaîne (AGLC) (nombre de carbones у 16) avec une très haute affinité, possède une structure de type récepteur avec une large poche hydrophobe extracellulaire flanquée de deux courts segments transmembranaires orientés dans le cytoplasme. Dans l'épithélium lingual chez la souris [5] et l'homme [6], l'expression du gène codant pour CD36 est strictement restreinte au bourgeon du goût. Quant à la protéine CD36, nous avons récemment montré qu'elle est abondamment exprimée sur la partie apicale de certaines cellules des bourgeons du goût (TRC, taste receptor cells) bordant le pore gustatif de la papille caliciforme chez la souris [5].…”

Section: Cd36 Un Récepteur De Lipides Impliqué Dans La Détection Orounclassified

STIM1, CD36 lingual et perception gustative lipidique

et al. 2012

View full text Add to dashboard Cite

Apical CD36 immunolocalization in human and porcine taste buds from circumvallate and foliate papillae

Cited by 120 publications

References 30 publications

The fatty acid translocase gene CD36 and lingual lipase influence oral sensitivity to fat in obese subjects

The fatty acid translocase gene CD36 and lingual lipase influence oral sensitivity to fat in obese subjects

The role of lipolysis in human orosensory fat perception

STIM1, CD36 lingual et perception gustative lipidique

Contact Info

Product

Resources

About