Aphid-repellent pheromone E-β-farnesene is generated in transgenic Arabidopsis thaliana over-expressing farnesyl diphosphate synthase2

Bhatia, Varnika; Maisnam, Jaya; Jain, Ajay; Sharma, Krishan Kumar; Bhattacharya, Ramcharan

doi:10.1093/aob/mcu250

Cited by 29 publications

(18 citation statements)

References 52 publications

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“…Similarly, Kos et al (2013) showed that transgenic Arabidopsis overproducing the terpenoids linalool, nerolidol and 4,8-dimethylnona-1,3,7-triene ((E)-DMNT) were less attractive to the aphid Brevicoryne brassicae L. (Hemiptera: Aphidae). Notably, b-farnesene is an alarm pheromone for aphids, probably facilitating pest dispersion and contributing to the anti-xenotic effect of the host (Bhatia et al, 2014). Some enzymes in the terpenoid pathway, such as b-ocimene synthase, which catalyse the biosynthesis of b-ocimene, are under control of the JA pathway (Zhang et al, 2009).…”

Section: Discussionmentioning

confidence: 99%

Tetranychus urticae‐triggered responses promote genotype‐dependent conspecific repellence or attractiveness in citrus

et al. 2015

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SummaryThe citrus rootstocks sour orange and Cleopatra mandarin display differential resistance against Tetranychus urticae. Sour orange plants support reduced oviposition, growth rates and damage compared with Cleopatra mandarin plants. Jasmonic acid signalling and flavonoid accumulation have been revealed as key mechanisms for the enhanced resistance of sour orange plants.In this study, we observed that the release of T. urticae herbivore-induced plant volatiles (HIPVs) from sour orange plants has a marked repellent effect on conspecific mites associated with the production of the terpenes a-ocimene, a-farnesene, pinene and D-limonene, and the green leaf volatile 4-hydroxy-4-methyl-2-pentanone. By contrast, T. urticae HIPVs from Cleopatra mandarin plants promote conspecific mite attraction associated with an increase in (2-butoxyethoxy) ethanol, benzaldehyde and methyl salicylate levels.HIPVs released from sour orange plants following T. urticae infestation induce resistance in Cleopatra mandarin plants, thereby reducing oviposition rates and stimulating the oxylipin biosynthetic gene lipoxygenase2 (LOX2). Cleopatra HIPVs do not affect the response to T. urticae of these rootstocks.We conclude that sour orange plants promote herbivore-induced resistance in Cleopatra mandarin plants and, despite the weak basal resistance of these rootstocks, herbivore resistance can be induced through the combination of HIPVs, such as a-ocimene and D-limonene.

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Section: Discussionmentioning

confidence: 99%

Tetranychus urticae‐triggered responses promote genotype‐dependent conspecific repellence or attractiveness in citrus

et al. 2015

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“…Two ATCOPIA93-containing genes, AT4G17190 and AT5G20280, which are implicated respectively in defense against aphids and in nectar secretion Bhatia et al 2015), also showed reduced expression, but only at generation F8. In both cases, ATCOPIA93 was located within an intron and either in the same or the opposite orientation relative to gene transcription and was associated with transcript truncation at F8, but not at F16 ( Figure 6C), when the insertions are epigenetically silent.…”

Section: Intronic Atcopia Insertions Create Epigenetically-inducible mentioning

confidence: 99%

Transposon accumulation lines uncover histone H2A.Z-driven integration bias towards environmentally responsive genes

Quadrana

Etcheverry

Gilly

et al. 2018

Preprint

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Inherited transposition events are important drivers of genome evolution but because transposable element (TE) mobilization is usually rare, its impact on the creation of genetic variation remains poorly characterized. Here, we used a population of A. thaliana epigenetic recombinant inbred lines (epiRILs) to characterize >8000 de novo insertions produced by several TEs families also active in nature. Integration was strongly biased towards genes, with evident deleterious effects. Biases were TE family-specific and associated with distinct chromatin features. Notably, we demonstrate that the histone variant H2A.Z guides the preferential integration of Ty1/Copia LTR-retrotransposons within environmentally responsive genes and that this guiding function is evolutionary conserved. Finally, we uncover an important role for epigenetic silencing in exacerbating or alleviating the effects of TE insertions on target genes. These findings establish chromatin as a major determinant of the spectrum and functional impact of TE-generated mutations, with important implications for adaptation and evolution. VC, PW and JMA conceived the project, with substantial additional input from LQ. ME and EC performed DNA and RNA extraction; JG, SE and JMA produced the WGS data; AG, ME, JMA and LQ performed the detection of TE insertions in the epiRILs; VB identified excision footprints in the epiRILs. LQ performed all of the other bioinformatic analyses, as well as the TE sequence capture and RT-

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“…C-C double bonds in prenyl-PP can be either in trans or cis conformation depending on the PT-trans-PTs form trans isomers, and cis-PTs form cis-isomers. Considering the length of the produced carbon backbone, trans-PTs can be classified as short-chain PTs (C 10-25 ), medium-chain PTs (C [30][31][32][33][34][35], and long-chain PTs (C 40-50 ) [7]. Members of the short-chain ones are in ascending order C 10 geranyl diphosphate synthase (GPPS), C 15 farnesyl diphosphate synthase (FPPS), C 20 geranylgeranyl diphosphate synthase (GGPPS), and C 25 geranylfarnesyl diphosphate synthase (GFPPS) ( Table 1).…”

Section: Trans-prenyltransferasesmentioning

confidence: 99%

“…The longer-variant FPPS1L is targeted to mitochondria, the shorter FPPS1S, together with the FPPS2, is localized to the cytosol. Research on this topic demonstrated that FPPS genes are redundant, thus one copy of the gene is enough to keep plants viable with only small changes in metabolite content [25,[30][31][32][33]. On the other hand, total loss of FPPS activity is lethal due to severely impaired embryo development [31].…”

Section: Trans-prenyltransferasesmentioning

confidence: 99%

Functional Gene Network of Prenyltransferases in Arabidopsis thaliana

Kopcsayová

Vranová

2019

Molecules

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Prenyltransferases (PTs) are enzymes that catalyze prenyl chain elongation. Some are highly similar to each other at the amino acid level. Therefore, it is difficult to assign their function based solely on their sequence homology to functional orthologs. Other experiments, such as in vitro enzymatic assay, mutant analysis, and mutant complementation are necessary to assign their precise function. Moreover, subcellular localization can also influence the functionality of the enzymes within the pathway network, because different isoprenoid end products are synthesized in the cytosol, mitochondria, or plastids from prenyl diphosphate (prenyl-PP) substrates. In addition to in vivo functional experiments, in silico approaches, such as co-expression analysis, can provide information about the topology of PTs within the isoprenoid pathway network. There has been huge progress in the last few years in the characterization of individual Arabidopsis PTs, resulting in better understanding of their function and their topology within the isoprenoid pathway. Here, we summarize these findings and present the updated topological model of PTs in the Arabidopsis thaliana isoprenoid pathway.

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Aphid-repellent pheromone E-β-farnesene is generated in transgenic Arabidopsis thaliana over-expressing farnesyl diphosphate synthase2

Cited by 29 publications

References 52 publications

Tetranychus urticae‐triggered responses promote genotype‐dependent conspecific repellence or attractiveness in citrus

Tetranychus urticae‐triggered responses promote genotype‐dependent conspecific repellence or attractiveness in citrus

Transposon accumulation lines uncover histone H2A.Z-driven integration bias towards environmentally responsive genes

Functional Gene Network of Prenyltransferases in Arabidopsis thaliana

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