Among the Hemiptera insects, a widespread way of feeding is sucking sap from host plants. Due to diet poor in nutrients, these insects enter into obligate symbiosis with their microorganisms. However, within the Cicadellidae family, there is a relatively large group of mesophyll feeders – Typhlocybinae that is considered to be devoid of symbiotic companions. In this work, we examine the composition of microorganisms in this subfamily and compare the results with their close relatives – the Eurymelinae subfamily. To study the microbiome, we used high-throughput next-generation sequencing (NGS, Illumina) and advanced microscopic techniques such as transmission electron microscopy (TEM) and fluorescencein situhybridization (FISH) in a confocal microscope. The Typhlocybinae insects have very poor microbial communities in their bodies, these are mainly facultative microorganisms, such as alphaproteobacteria of the genusWolbachiaorRickettsia. We detected also the presence of bacteria that can be considered as facultative symbionts e.g.Spiroplasma, Acidocella, Arsenophonus, Sodalis, Lariskella, Serratia, CardiniumandAsaia. On the other hand, the Eurymelinae group is characterized by a large diversity of the microbial communities, similar to those described in other Cicadomorpha. We find obligate co-symbionts involved in the synthesis of essential amino acids such asSulcia, betaproteobacteria related to genusNasuiaor gammaproteobacteriaSodalis. In other representatives, we observed symbiotic yeast-like fungi from the family Ophiocordycipitaceae and within some genera we discoveredArsenophonusbacteria inhabiting the interior ofSulciabacteria. Additionally, we investigated the transovarial transmission of obligate symbionts, which occurs via infection of the ovaries of females.