Apathogenic, intestinal, segmented, filamentous bacteria stimulate the mucosal immune system of mice

Klaasen, H. L. B. M.; Heijden, P.J. van der; Stok, W.; Poelma, F. G. J.; Koopman, J. P.; Brink, Monique; Bakker, Marinka A.H.; Eling, W. M. C.; Beynen, A.C.

doi:10.1128/iai.61.1.303-306.1993

Cited by 201 publications

(97 citation statements)

References 13 publications

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“…Monoassociation with segmented filamentous bacteria (SFB), which are indigenous endospore-forming bacteria strongly bound to the ileal epithelial cells, increased the number of abT cell receptor (TCR)-bearing IELs (abIEL), the mitotic activity and ratio of the number of columnar cells to goblet cells and induced MHC class II molecules and fucosyl asialo GM1 glycolipids in the small intestinal epithelial cells (Umesaki et al 1995). Moreover, SFB are shown to interact with intraepithelial mononuclear cells in Peyer's patches (Meyerholz et al 2002) and to stimulate IgA production (Klassen et al 1993;Talham et al 1999). Snel et al (1995) proposed a new taxnomic position for the SFB based on 16S rDNA sequence analysis.…”

Section: Introductionmentioning

confidence: 99%

Improvement of human faecal flora-associated mouse model for evaluation of the functional foods

et al. 2004

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Aims: Animal models are required for evaluation of the functional foods such as pro/prebiotics exerting effects through the metabolism of the intestinal microflora. The object of this study was to establish new human floraassociated mice reflecting the environment of the human intestinal tract. Methods and Results: We inoculated a human faecal suspension into segmented filamentous bacteria (SFB) monoassociated mice as a model system. In both human flora (HF) and SFB-associated mouse (HF-SFB mouse), intestinal characteristics such as the composition of intraepithelial lymphocytes, the expression of major histocompatibility complex (MHC) class II molecules and the number of immunoglobulin A-producing cells in the mucosa was closer to those of conventionally reared mice than was case with human flora-associated mice (HF mice) lacking SFB. Several predominant bacterial groups except lactobacilli in human flora were found in faeces of HF-SFB mice. Lactobacilli established small populations in the gut of HF-SFB mice when administered before inoculation with the human flora. Faecal enzymatic activities and organic acid concentration of HF-SFB mice proportionally reflected those of the donor subject. Conclusion: We established a new human flora-associated mouse (HF-SFB mouse), in which intestinal characteristics are normally developed and their major microbial composition reflect the human. Significance and Impact of the Study: HF-SFB mice are a valuable model for studying pro/prebiotic effects on the human intestine.

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Section: Introductionmentioning

confidence: 99%

Improvement of human faecal flora-associated mouse model for evaluation of the functional foods

et al. 2004

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“…increase in the mucosal immune system (Klaasen et al 1993;Umesaki et al 1995). No visible abnormalities were observed in any of the organs that have been studied.…”

Section: Discussionmentioning

confidence: 89%

The ability of nisin F to control Staphylococcus aureus infection in the peritoneal cavity, as studied in mice

Brand¹,

Kwaadsteniet²,

Dicks³

2010

Letters in Applied Microbiology

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Aims: To determine the ability of nisin F to control systematic infection caused by Staphylococcus aureus, using C57BL/6 mice as a model. Methods and Results: Twelve mice were intraperitoneally injected with 1 × 108 viable cells of Staph. aureus Xen 36 containing the modified Photorhabdus luminescence luxABCDE operon on plasmid pAUL‐A Tn4001. After 4 h, six mice were intraperitoneally injected with 640 arbitrary units (AU) nisin F, and six were injected with sterile saline. Six mice, not infected with Staph. aureus, were treated with nisin F, and six not infected were left untreated. The viability of Staph. aureus Xen 36 was monitored over 48 h by recording photon emission levels. Nisin F suppressed Staph. aureus for 15 min in vivo. No abnormalities were recorded in blood analyses and internal organs of mice treated with nisin F. Conclusions: Nisin F suppressed the growth of Staph. aureus in the peritoneal cavity for at least 15 min. Re‐emergence of Staph. aureus bioluminescence over the next 44 h suggests that nisin F was inactivated, most probably by proteolytic enzymes. Significance and Impact of the Study: A single dosage of nisin F administered in the peritoneal cavity controlled the growth of Staph. aureus for at least 15 min in vivo.

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“…The presence of intestinal microbes directly facilitates the infection of reoviruses and polioviruses, both in vitro and in vivo (Kuss et al, 2011). The bacterial flora promotes maturation of secondary lymphoid organs in the intestine (Lee and Mazmanian, 2010) and can influence both gene expression in antigen-presenting cells and the way T cells respond to vaccines (Klaasen et al, 1993;Lamousé -Smith et al, 2011). Furthermore, interactions of macrophages with probiotic bacteria lead to increased antiviral response against vesicular stomatitis virus in vitro (Ivec et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

Recombinant feline coronaviruses as vaccine candidates confer protection in SPF but not in conventional cats

Bálint

Farsang

Szeredi

et al. 2014

Veterinary Microbiology

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Feline infectious peritonitis virus (FIPV) is a major pathogen of Felidae. Despite the extensive efforts taken in the past decades, development of the "ideal" live attenuated FIPV vaccine was not successful yet. In the present study, we provide data of immunisation experiments with a recombinant FCoV pair differing only in the truncation (PBFIPV-DF-2) or completion (PBFIPV-DF-2-R3i) of their ORF3abc regions. In our previous in vivo studies, these viruses proved to show the characters of low virulent or avirulent FCoV phenotypes, respectively. Therefore, we hypothesised the ability of these viruses, as possible vaccine candidates, in conferring protection in specific pathogen free (SPF) Domestic Shorthair as well as in conventional purebred British Shorthair cats. In SPF cats, after two oronasal and two intramuscular vaccinations with two weeks intervals, both vaccine candidates provided 100% protection against lethal homologous challenge with the highly virulent FIPV DF-2 strain. In contrast, the conventional purebred British Shorthair cats did not develop protection when they were immunised with the same vaccination regimes. In these groups 100% of the PBFIPV-DF-2-R3i immunised animals developed antibody-dependent enhancement (ADE). Prolonged survival was observed in 40% of the animals, while 60% showed fulminant disease course. Genetic and more probably immunological differences between the SPF and non-SPF purebred kittens can explain the different outcome of the vaccination experiment. Our data highlight the diverse immune responses between SPF and conventional cats and suggest a decisive role of previous infection by heterologous causative agents in the outcome of the vaccination against FIP.

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Apathogenic, intestinal, segmented, filamentous bacteria stimulate the mucosal immune system of mice

Cited by 201 publications

References 13 publications

Improvement of human faecal flora-associated mouse model for evaluation of the functional foods

Improvement of human faecal flora-associated mouse model for evaluation of the functional foods

The ability of nisin F to control Staphylococcus aureus infection in the peritoneal cavity, as studied in mice

Recombinant feline coronaviruses as vaccine candidates confer protection in SPF but not in conventional cats

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