AP2/ERF transcription factor GmDREB1 confers drought tolerance in transgenic soybean by interacting with GmERFs

Chen, Kai; Tang, Wensi; Zhou, Yongbin; Chen, Jun; Xu, Zhao‐Shi; Ma, Rui; Dong, Yingshan; Ma, You-Zhi; Chen, Ming

doi:10.1016/j.plaphy.2021.12.014

Cited by 37 publications

(24 citation statements)

References 31 publications

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“…ERF1-V in wheat ( Heuchera villosa ) improved drought tolerance via modulating P5CR and OAT involved in the proline synthesis ( Xing et al., 2017 ). GmDREB1 could confer drought tolerance of soybean by increasing the photosynthetic efficiency, the accumulation of osmoregulation substances, and the synthesis of melatonin ( Chen K. et al., 2022 ). On the other hand, ERFs could also enhance the ROS scavenging ability of plants under drought condition.…”

Section: Erfs Regulate Plant Responses To Various Abiotic Stressesmentioning

confidence: 99%

“…In this classification system, the AP2 subfamily contains two highly similar AP2 domains, while the ERF and DREB subfamilies each contains a single AP2 domain. Proteins of the DREB subfamily contain conserved amino acid residues at 14 (Val) and 19 (Glu) of the AP2/ERF domains, while those of ERF subfamily are Ala and Asp at position-14 and position-19 ( Chen K. et al., 2022 ). In addition, the RAV subfamily contains an AP2 structure and a B3 structure, the Soloist subfamily contains an AP2 structural domain that differs significantly from those of other ERF transcription factors ( Feng et al., 2020 ).…”

Section: Introductionmentioning

confidence: 99%

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ERF subfamily transcription factors and their function in plant responses to abiotic stresses

Zhang

et al. 2022

Front. Plant Sci.

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Ethylene Responsive Factor (ERF) subfamily comprise the largest number of proteins in the plant AP2/ERF superfamily, and have been most extensively studied on the biological functions. Members of this subfamily have been proven to regulate plant resistances to various abiotic stresses, such as drought, salinity, chilling and some other adversities. Under these stresses, ERFs are usually activated by mitogen-activated protein kinase induced phosphorylation or escape from ubiquitin-ligase enzymes, and then form complex with nucleic proteins before binding to cis-element in promoter regions of stress responsive genes. In this review, we will discuss the phylogenetic relationships among the ERF subfamily proteins, summarize molecular mechanism how the transcriptional activity of ERFs been regulated and how ERFs of different subgroup regulate the transcription of stress responsive genes, such as high-affinity K+ transporter gene PalHKT1;2, reactive oxygen species related genes LcLTP, LcPrx, and LcRP, flavonoids synthesis related genes FtF3H and LhMYBSPLATTER, etc. Though increasing researches demonstrate that ERFs are involved in various abiotic stresses, very few interact proteins and target genes of them have been comprehensively annotated. Hence, future research prospects are described on the mechanisms of how stress signals been transited to ERFs and how ERFs regulate the transcriptional expression of stress responsive genes.

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Section: Erfs Regulate Plant Responses To Various Abiotic Stressesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

ERF subfamily transcription factors and their function in plant responses to abiotic stresses

Zhang

et al. 2022

Front. Plant Sci.

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“…Additionally, AtERF95 interacts with AtERF97 to enhance heat stress tolerance (Huang et al ., 2021 ). In transgenic soybean, drought tolerance was improved by the interaction between GmDREB1 and GmERF008 or GmERF106 (Chen et al ., 2022 ). However, the protein–DNA interactions among different ERF factors have not been explored.…”

Section: Discussionmentioning

confidence: 99%

CmERF5‐CmRAP2.3 transcriptional cascade positively regulates waterlogging tolerance in Chrysanthemum morifolium

Zhao

et al. 2022

Plant Biotechnology Journal

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Waterlogging stress affects plant growth by limiting root respiration and reducing yield and economic value. Therefore, identifying genes involved in regulating waterlogging stress is vital. This study reports the ethylene-responsive VII transcription factor (CmRAP2.3) in the chrysanthemum. Subcellular localization and transactivation assay analyses revealed that CmRAP2.3 was localized in the nucleus and possessed transactivation activity. Overexpression of CmRAP2.3 in chrysanthemum was found to enhance waterlogging tolerance by decreasing reactive oxygen species (ROS) levels. Furthermore, we found that the transcription factor CmERF5 binds to GCC-like motifs in the CmRAP2.3 promoter region and activates CmRAP2.3 expression. Additionally, CmERF5 overexpression maintained a low ROS level and improved chrysanthemum waterlogging tolerance. Taken together, this study shows a molecular mechanism by which CmERF5 transcriptionally activates CmRAP2.3 to reduce waterlogging stress via the ROS pathway in the chrysanthemum.

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“…To measure plant fresh weight, soil was washed off of the treated plants, after which excess water was removed with absorbent paper, and plants were weighed with a BSA224S-CW 1/10,000 analytical balance (Sartorius, Beijing, China). Three biological replicate samples were analyzed for these assays (Chen et al, 2022).…”

Section: Measurements Of Root Length and Plant Weightmentioning

confidence: 99%

The Alfin-like transcription factor GmAlfin09 regulates endoplasmic reticulum stress in soybean via a peroxidase GmPRDX6

Chen¹,

Guo²,

Yan³

et al. 2023

Preprint

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Soybean is a valuable oil crop cultivated throughout the world, but also highly susceptible to environmental stressors. The development of approaches to enhancing soybean stress resistance is thus vital to improving yields. In prior studies, Alfin has been shown to serve as an epigenetic regulator of plant growth and development. No studies of Alfin have yet been reported in soybean, however. In this study, the endoplasmic reticulum (ER) stress- and reactive oxygen species (ROS)-related transcription factor GmAlfin09 was identified. Screening of genes co-expressed with GmAlfin09 unexpectedly led to the identification of the peroxidase GmPRDX6. Further analyses revealed that both GmAlfin09 and GmPRDX6 were responsive to ER stress, with GmPRDX6 localizing to the ER under stress. Promoter binding experiments confirmed the ability of GmAlfin09 to directly bind the GmPRDX6 promoter. When GmAlfin09 and GmPRDX6 were overexpressed in soybean, enhanced ER stress resistance and decreased ROS levels were observed. Together, these findings suggest that GmAlfin09 can promote the upregulation of GmPRDX6, which subsequently localizes to the ER, reduced ROS levels, promotes ER homeostasis, and helps ensure the normal growth of soybean even under ER stress. This study highlights a novel genetic target for the future molecular breeding of stress-resistant soybean cultivars.

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AP2/ERF transcription factor GmDREB1 confers drought tolerance in transgenic soybean by interacting with GmERFs

Cited by 37 publications

References 31 publications

ERF subfamily transcription factors and their function in plant responses to abiotic stresses

ERF subfamily transcription factors and their function in plant responses to abiotic stresses

CmERF5‐CmRAP2.3 transcriptional cascade positively regulates waterlogging tolerance in Chrysanthemum morifolium

The Alfin-like transcription factor GmAlfin09 regulates endoplasmic reticulum stress in soybean via a peroxidase GmPRDX6

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