AP-2&amp;alpha; expression in papillary thyroid carcinoma predicts tumor progression and poor prognosis

Wu, Hongrong; Zhang, Jian

doi:10.2147/cmar.s167874

Cited by 12 publications

(7 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…ChIP results con rmed the prediction. AP-2α was found to be highly expressed in PTC tissues, and the high expression of AP-2α mRNA was signi cantly correlated with advanced tumor stage and shorter overall survival 43 . Knock-down of AP-2α inhibited proliferation in PTC cells.…”

Section: Discussionmentioning

confidence: 96%

Genetic predisposition to papillary thyroid carcinoma is mediated by a long non-coding RNA TINCR enhancer polymorphism

Wang

Huang

Chen

et al. 2021

Preprint

View full text Add to dashboard Cite

Single nucleotide polymorphisms (SNPs) in the enhancer region have been demonstrated to confer to altered enhancer activities, aberrant gene expression, and cancer susceptibility. In this study, we aimed to examine the association between an SNP, rs8101923, within terminal differentiation-induced non-coding RNA (TINCR) and the risk of papillary thyroid carcinoma (PTC). Blood samples from 559 patients with PTC and 445 healthy individuals were collected. The rs8101923 was genotyped by using polymerase chain reaction-restriction fragment length polymorphism assay. The impact of the rs8101923 on TINCR expression and enhancer activity was evaluated by quantitative real-time PCR and dual-luciferase reporter assay. The binding of AP-2α to TINCR enhancer was determined by chromatin immunoprecipitation. The rs8101923 G allele was significantly associated with a higher risk of PTC (adjusted OR = 1.37; 95% CI: 1.15–1.64). Mechanistically, the rs8101923 was related to increased transcriptional levels and enhancer activities (P < 0.05). Transcription factor AP-2α binds to the enhancer region of TINCR containing the rs8101923 locus, and promotes cell proliferation in PTC. These findings suggest the rs8101923 as a risk factor in the pathogenesis of PTC, which provides evidence for explaining the mechanism of the rs8101923 risk allele predisposing to PTC.

show abstract

Section: Discussionmentioning

confidence: 96%

Genetic predisposition to papillary thyroid carcinoma is mediated by a long non-coding RNA TINCR enhancer polymorphism

Wang

Huang

Chen

et al. 2021

Preprint

View full text Add to dashboard Cite

show abstract

“…Additionally, our data demonstrated that AP2α expression presents a high prognostic value for CC. Wu and Zhang (2018) [ 19 ] showed in papillary thyroid carcinoma (PTC) that AP2α overexpression was significantly associated with tumor stage, histologic type, and independently predicted shorter OS. Patients with TFAP2α overexpression had a shorter OS than AP2α down-expression, particularly in PTC patients with advanced tumor stages (III and IV).…”

Section: Discussionmentioning

confidence: 99%

Identification of Mir-182-3p/FLI-1 Axis as a Key Signaling in Immune Response in Cervical Cancer: A Comprehensive Bioinformatic Analysis

Salmerón-Bárcenas

Mendoza-Catalán

Ramírez-Bautista

et al. 2023

IJMS

View full text Add to dashboard Cite

miRNAs modulate gene expression and play critical functions as oncomiRs or tumor suppressors. The miR-182-3p is important in chemoresistance and cancer progression in breast, lung, osteosarcoma, and ovarian cancer. However, the role of miR-182-3p in cervical cancer (CC) has not been elucidated. Aim: To analyze the role of miR-182-3p in CC through a comprehensive bioinformatic analysis. Methods: Gene Expression Omnibus (GEO) databases were used for the expression analysis. The mRNA targets of miR-182-3p were identified using miRDB, TargetScanHuman, and miRPathDB. The prediction of island CpG was performed using the MethPrimer program. The transcription factor binding sites in the FLI-1 promoter were identified using ConSite+, Alibaba2, and ALGGEN-PROMO. The protein–protein interaction (PPI) analysis was performed in STRING 11.5. Results: miR-182-3p was significantly overexpressed in CC patients and has potential as a diagnostic. We identified 330 targets of miR-182-3p including FLI-1, which downregulates its expression in CC. Additionally, the aberrant methylation of the FLI-1 promoter and Ap2a transcription factor could be involved in downregulating FLI1 expression. Finally, we found that FLI-1 is a possible key gene in the immune response in CC. Conclusions: The miR-182-3p/FLI-1 axis plays a critical role in immune response in CC.

show abstract

“…Indeed, both AP-2α and AP-2γ have prognostic value for some tumor types. AP-2α overexpression is recognized as a prognostic indicator of shorter patient survival in papillary thyroid carcinoma and epithelial ovarian cancer but longer survival in gastric adenocarcinoma [20][21][22]. In terms of AP-2γ, its overexpression was also associated with poorer overall survival in breast cancer patients [23] with the other literature data to support this statement and enrich it with correlation presenting worse anti-hormone therapy response [24].…”

Section: Introductionmentioning

confidence: 92%

Functional genomics of AP-2α and AP-2γ in cancers: in silico study

et al. 2020

View full text Add to dashboard Cite

Background Among all causes of death, cancer is the most prevalent and is only outpaced by cardiovascular diseases. Molecular theory of carcinogenesis states that apoptosis and proliferation are regulated by groups of tumor suppressors or oncogenes. Transcription factors are example of proteins comprising representatives of both cancer-related groups. Exemplary family of transcription factors which exhibits dualism of function is Activating enhancer-binding Protein 2 (AP-2). Scientific reports concerning their function in carcinogenesis depend on particular family member and/or tumor type which proves the issue to be unsolved. Therefore, the present study examines role of the best-described AP-2 representatives, AP-2α and AP-2γ, through ontological analysis of their target genes and investigation what processes are differentially regulated in 21 cancers using samples deposited in Genomic Data Analysis Center (GDAC) Firehose. Methods Expression data with clinical annotation was collected from TCGA-dedicated repository GDAC Firehose. Transcription factor targets were obtained from Gene Transcription Regulation Database (GTRD), TRANScription FACtor database (TRANSFAC) and Transcriptional Regulatory Relationships Unraveled by Sentence-based Text mining (TRRUST). Monocle3 R package was used for global samples profiling while Protein ANalysis THrough Evolutionary Relationships (PANTHER) tool was used to perform gene ontology analysis. Results With RNA-seq data and Monocle3 or PANTHER tools we outlined differences in many processes and signaling pathways, separating tumor from normal tissues or tumors from each other. Unexpectedly, a number of alterations in basal-like breast cancer were identified that distinguished it from other subtypes, which could bring future clinical benefits. Conclusions Our findings indicate that while the AP-2α/γ role remains ambiguous, their activity is based on processes that underlie the cancer hallmarks and their expression could have potential in diagnosis of selected tumors.

show abstract

AP-2α expression in papillary thyroid carcinoma predicts tumor progression and poor prognosis

Cited by 12 publications

References 22 publications

Genetic predisposition to papillary thyroid carcinoma is mediated by a long non-coding RNA TINCR enhancer polymorphism

Genetic predisposition to papillary thyroid carcinoma is mediated by a long non-coding RNA TINCR enhancer polymorphism

Identification of Mir-182-3p/FLI-1 Axis as a Key Signaling in Immune Response in Cervical Cancer: A Comprehensive Bioinformatic Analysis

Functional genomics of AP-2α and AP-2γ in cancers: in silico study

Contact Info

Product

Resources

About