Aortic valve graft implantation in rats: a new functional model

Légaré, Jean‐François; Nanton, Maurice A.; Bryan, Patricia; Lee, Timothy D.G.

doi:10.1067/mtc.2000.109239

Cited by 14 publications

(4 citation statements)

References 20 publications

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“…Of note, these techniques are limited by placing donor valve in series with the recipients' native aortic valve. 21 This results in abnormal blood flow patterns and removes the pressure gradient necessary to open and close the donor valve. 22 In order to avoid size mismatch of the anastomoses during implantation, Lee et al developed a rodent model where a donor pulmonary valve is transplanted orthotopically into a second donor heart.…”

Section: He Terotopi C Partial He Art Tr Ans Pl Antation In the Ab Do...mentioning

confidence: 99%

“…Therefore, Yankah's technique was refined by Green et al, 19 who used the innominate artery instead of the ascending aorta to create the distal anastomosis, and Zhou et al, 20 anastomosed the donor aortic arch to the aortic annulus, so the subclavian artery can be used for the proximal anastomosis. Of note, these techniques are limited by placing donor valve in series with the recipients' native aortic valve 21 . This results in abnormal blood flow patterns and removes the pressure gradient necessary to open and close the donor valve 22 .…”

Section: Heterotopic Partial Heart Transplantation In the Abdominal A...mentioning

confidence: 99%

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Animal models for partial heart transplantation

Yerger,

Hill,

Fitzgerald

et al. 2024

Pediatric Transplantation

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BackgroundPartial heart transplantation delivers growing heart valve implants by transplanting the part of the heart containing the necessary heart valve only. In contrast to heart transplantation, partial heart transplantation spares the native ventricles. This has important implications for partial heart transplant biology, including the allowable ischemia time, optimal graft preservation, primary graft dysfunction, immune rejection, and optimal immunosuppression.AimsExploration of partial heart transplant biology will depend on suitable animal models. Here we review our experience with partial heart transplantation in rodents, piglets, and non‐human primates.Materials & MethodsThis review is based on our experience with partial heart transplantation using over 100 rodents, over 50 piglets and one baboon.ResultsSuitable animal models for partial heart transplantation include rodent heterotopic partial heart transplantation, piglet orthotopic partial heart transplantation, and non‐human primate partial heart xenotransplantation.DiscussionRodent models are relatively cheap and offer extensive availability of research tools. However, rodent open‐heart surgery is technically not feasible. This limits rodents to heterotopic partial heart transplant models. Piglets are comparable in size to children. This allows for open‐heart surgery using clinical grade equipment for orthoptic partial heart transplantation. Piglets also grow rapidly, which is useful for studying partial heart transplant growth. Finally, nonhuman primates are immunologically most closely related to humans. Therefore, nonhuman primates are most suitable for studying partial heart transplant immunobiology and xenotransplantation.ConclusionsAnimal research is a privilege that is contingent on utilitarian ethics and the 3R principles of replacement, reduction and refinement. This privilege allows the research community to seek fundamental knowledge about partial heart transplantation, and to apply this knowledge to enhance the health of children who require partial heart transplants.

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Section: He Terotopi C Partial He Art Tr Ans Pl Antation In the Ab Do...mentioning

confidence: 99%

Section: Heterotopic Partial Heart Transplantation In the Abdominal A...mentioning

confidence: 99%

Animal models for partial heart transplantation

Yerger,

Hill,

Fitzgerald

et al. 2024

Pediatric Transplantation

View full text Add to dashboard Cite

show abstract

“…1 To overcome this difficulty Légaré and Ross suggest the use of a functional rat implantation model in which the creation of native aortic valve insufficiency results in a significant diastolic flow reversal in the abdominal aorta, allowing leaflets to move and function. 2 Although we were aware of the existence of this model, we decided not to use it for our study. One reason is that the creation of aortic insufficiency results in progressive congestive heart failure from volume overload, making the model unsuitable for long-term follow-up studies.…”

Section: Reply To the Editormentioning

confidence: 99%

“…In a previous article, they reported performing coronary artery bypass grafting first, then MVR, and finally LVR. 2 Ischemic mitral regurgitation is a functional disorder that is due mainly to changes in the geometric relationships between structurally normal valve components and a severely damaged ventricle. By changing ventricular volume and geometry, LVR may unpredictably result in mitral insufficiency that is absent before surgery 3 or in an amelioration (sometimes disappearance) of mitral regurgitation.…”

Section: Which Is the Best Surgical Strategy For "Complete" Treatment Of Ischemic Cardiomyopathy? To The Editormentioning

confidence: 99%

Reply to the Editor:

Grauss

DeRuiter

Hazekamp

2004

The Journal of Thoracic and Cardiovascular Surgery

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Comparison of aortic valve allograft decellularization techniques in the rat

Meyer

Chiu

Churchill

et al. 2006

J Biomedical Materials Res

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151

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Rodent models have been essential to understanding the immune-mediated failure of aortic valve allografts (AVAs). Decellularization has been proposed to reduce the immunogenicity of AVAs. The objective of this study was to determine the most effective method to decellularize AVAs for use in a rat model. Three different decellularization techniques were compared in Lewis aortic valves. Detergent decellularization involved a series of hypotonic and hypertonic Tris buffers at 4 degrees C for 48 h/buffer containing 0.5% Triton X-100 followed by a 72 h washout in phosphate-buffered saline. Osmotic decellularization was performed in similar manner to the detergent-based technique except without the addition of Triton X-100. Enzymatic decellularization consisted of trypsin/EDTA at 37 degrees C for 48 h. Assessment was performed with light microscopy (H&E, Movat's pentachrome), immunohistochemistry for residual cellular elements, and hydroxyproline assays. Detergent-based methodology effected near-complete decellularization of both the leaflets and aortic wall in addition to preservation of the extracellular matrix (ECM). Osmotic lysis was associated with preservation of ECM and moderate decellularization. Enzymatic decellularization resulted in complete decellularization but extensive degeneration and fragmentation of the ECM. When implanted into the infrarenal aorta of allogeneic rats for 1 week, valves decellularized with detergent-based and osmotic methodology failed to stimulate an allogeneic immune response as evidenced by an absence of T cell infiltrates. Osmotic lysis protocols with low dose detergent appear to be most effective at both removing antigenic cellular elements and preserving ECM.

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Aortic valve graft implantation in rats: a new functional model

Cited by 14 publications

References 20 publications

Animal models for partial heart transplantation

Animal models for partial heart transplantation

Reply to the Editor:

Comparison of aortic valve allograft decellularization techniques in the rat

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