Anxiolytic properties of Cymbopogon citratus (DC.) stapf extract, essential oil and its constituents in zebrafish (Danio rerio)

Hacke, Ana Carolina Mendes; Miyoshi, Edmar; Pereira, Romaiana Picada

doi:10.1016/j.jep.2020.113036

Cited by 44 publications

(31 citation statements)

References 62 publications

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“…The zebrafish model is widely used in toxicity assays; however, to our knowledge, this is the first report on the toxicity of different kind of EOs from Cymbopogon based on this model. Besides two scientific reports [30,31] about Cymbopogon activity on the central nervous system, no study to date has been published on the activity of Cymbopogon on any zebrafish model. However, there are many reports about the anti-inflammatory or neuroprotective activity of different EOs, such as thyme or rosemary essential oils [49][50][51].…”

Section: Discussionmentioning

confidence: 99%

“…Pioneering studies suggest that chemicals can, in many cases, have very similar toxicological and teratological effects in zebrafish embryos and humans [28]. The effect of C. citratus EO on zebrafish has been examined in some research, in which promising activity on the central nervous system, i.e., anxiolytic, anticonvulsant and neuroprotective effects, were reported [30,31].…”

Section: Introductionmentioning

confidence: 99%

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Composition, Anti-MRSA Activity and Toxicity of Essential Oils from Cymbopogon Species

et al. 2021

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Many of the essential oils obtained from medicinal plants possess proven antimicrobial activity and are suitable for medicinal purposes and applications in the food industry. The aim of the present work was the chemical analysis of 19 essential oils (EOs) from seven different Cymbopogon species (C. nardus, C. citratus, C winterianus, C. flexuosus, C. schoenanthus, C. martinii, C. giganteus). Five different chemotypes were established by GC/MS and TLC assay. The EOs, as well as some reference compounds, i.e., citronellol, geraniol and citral (neral + geranial), were also tested for their antimicrobial and antibiofilm activity against methicillin-resistant Staphylococcus aureus (MRSA) by the microdilution method and direct bioautography. The toxicity of EOs was evaluated by Danio rerio ‘Zebrafish’ model assay. All examined EOs showed moderate to high activity against MRSA, with the highest activity noted for C. flexuosus—lemongrass essential oil, both in microdilution and direct autobiography method. Significant difference in the toxicity of the examined EOs was also detected.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Composition, Anti-MRSA Activity and Toxicity of Essential Oils from Cymbopogon Species

et al. 2021

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“…As expected, animals exposed to clonazepam and fluoxetine, which are drugs consolidated in the treatment of anxiety disorders, had a significantly longer dwelling time in the clear compartment than those that were not exposed. Hacke, Miyoshi, Marques, & Pereira (2020) also used clonazepam as a positive control in her study at the same concentration of (0.75 mg/L) and similarly observed an increase in time spent on the light side of the tank compared to the untreated group. Magno, Fontes, Gonçalves, & Gouveia (2015) obtained a similar result when exposed zebrafish to clonazepam 0.006 mg/L for 600 seconds, reporting that exposure decreased the time in the dark, but acute exposure with fluoxetine at concentrations of 2, 4 and 6 mg/L did not decrease this parameter.…”

Section: Discussionmentioning

confidence: 88%

Anxiolytic activity of ferulic acid in the light-dark test in zebrafish

Sborgi

Fernandes

Santos

et al. 2021

RSD

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The anxiety disorders belong to a group of mental disorders in which the patients present excessive fear and worry. Studies with ferulic acid have shown positive results on treating depressive symptoms. As many antidepressive drugs are effective in treating anxiety, the objective of the present study was to evaluate ferulic acid’s anxiolytic activity and possible mechanism of action in the light-dark test in zebrafish. To evaluate anxiolytic activity, the light-dark preference test was performed after exposure of the animals to ferulic acid or positive control (clonazepam or fluoxetine). Ferulic acid increased the time spent in the clear compartment at concentrations of 250 and 500 mg/L, not differing from the groups exposed to clonazepam or fluoxetine. To evaluate the possible mechanism of action, pre-exposure to flumazenil was carried out, followed by exposure to ferulic acid or positive control, with subsequent testing. Pre-exposure to flumazenil caused a significant reduction in the time spent in the clear compartment of ferulic acid and clonazepam groups but did not alter the effect of exposure to fluoxetine. These results suggest that ferulic acid promotes an anxiolytic effect, possibly through an action at the benzodiazepine binding site at the GABAA receptor.

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“…The Locomotor Behavior of Larvae. Previous studies have shown that DZP and MACB at drinking water pollution level interfere with the neurodevelopment of juvenile fish; we further explored the behavioral effect of DZP and MACB on the larval fish using the dark-light test [32,33]. The eyes of larvae could detect light signals and made a judgement [34].…”

Section: Assessment Of Impact On Nervous Developmentmentioning

confidence: 99%

Diazepam and Its Disinfection Byproduct Promote the Early Development of Nervous System in Zebrafish Embryos

Zhao

Huang

Shi

et al. 2020

Oxidative Medicine and Cellular Longevity

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The widely used diazepam, as central nervous system inhibitor, has found to be ubiquitous in surface water and drinking water. Moreover, a series of byproducts such as 2-methylamino-5-chlorobenzophenone (MACB) were generated after the chlorine disinfection process. However, little information is available about the neurobiological effects of these emerging chemicals at low doses, especially on infants and children. Here, we exposed zebrafish (Danio rerio) embryos to diazepam and MACB at 0.05, 0.5, and 5 nM, which were equivalent to environmental levels. Both diazepam and MACB increased the somite number and promoted nervous development of transgenic zebrafish [Tg (elavl3: EGFP) larvae] at 72 hours postfertilization ( hpf). Both diazepam and MACB also disrupted the homeostasis of adenosine monophosphate, valine, methionine, and fumaric acid in zebrafish embryos at 12 hpf. Additionally, the locomotor behavior activity of zebrafish was significantly enhanced after 120-hour sustained exposure to diazepam or MACB. Moreover, the mRNA expression levels of oct4, sox2, and nanog, modulating the pluripotency and self-renewal, were upregulated by diazepam and MACB in zebrafish embryo. Altogether, diazepam and MACB stimulate developmental neurogenesis and may induce neuronal excitotoxicity at quite low doses. These results indicated that the chronic exposure to psychoactive drugs may pose a potential risk to the development of the nervous system in infancy.

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Anxiolytic properties of Cymbopogon citratus (DC.) stapf extract, essential oil and its constituents in zebrafish (Danio rerio)

Cited by 44 publications

References 62 publications

Composition, Anti-MRSA Activity and Toxicity of Essential Oils from Cymbopogon Species

Composition, Anti-MRSA Activity and Toxicity of Essential Oils from Cymbopogon Species

Anxiolytic activity of ferulic acid in the light-dark test in zebrafish

Diazepam and Its Disinfection Byproduct Promote the Early Development of Nervous System in Zebrafish Embryos

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