Anxiolytic effects of γ-oryzanol in chronically- stressed mice are related to monoamine levels in the brain

Akter, Salina; Sasaki, Hiroyuki; Uddin, Kazi Rasel; Ikeda, Yuko; Miyakawa, Hiroki; Shibata, Shigenobu

doi:10.1016/j.lfs.2018.11.042

Cited by 18 publications

(25 citation statements)

References 44 publications

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“…We previously demonstrated that 0.5% GORZ may act as a promising anxiolytic functional food in chronic restraint stressed mice, via regulation of central monoamine neurotransmitters in the amygdala (Akter et al, 2019). However, the antiinflammatory effect of GORZ, along with its anxiolytic function, also served as areas of focus in the current study.…”

Section: Discussionmentioning

confidence: 84%

“…For preparation of the HFD + GORZ food, GORZ was obtained from Sigma-Aldrich (St. Louis, Missouri, USA). A GORZ ratio of 0.5% was selected based on our existing study (Akter et al, 2019), fixed with the weight of HFD, and administered to mice. Mice were allowed free access to food and tap water during the experimental period, and exposure time was the same for all groups of mice.…”

Section: Treatmentsmentioning

confidence: 99%

“…The cerebral cortices (mean wet weight, 14.55 mg) were dissected free-hand from the upper regions of the dorsal hippocampus. Detailed protocol for slicing and dissecting brain areas of interest according to the brain atlas (from bregma -0.5 to bregma -2.5) is described in our previous paper (Akter et al, 2019). Brain tissue was moved to a -80°C freezer and stored at -80°C until further analysis.…”

Section: Animal Sacrifice and Sample Collectionmentioning

confidence: 99%

“…EPC-300 software (version 2.5.10, Eicom) was used to evaluate the data. The applied protocol is published in detail in our existing paper (Akter et al, 2019).…”

Section: High-performance Liquid Chromatography-electrochemical Detecmentioning

confidence: 99%

“…Recent data from our existing research suggest the anxiolytic mechanism of GORZ as being related to brain levels of monoamines in the amygdala of the mouse brain, as observed in chronic restraint stressed mice (Akter et al, 2019). However, the antiinflammatory effect of GORZ, alongside its anxiolytic role, have to date not been analyzed.…”

Section: Introductionmentioning

confidence: 99%

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Gamma Oryzanol Alleviates High-Fat Diet-Induced Anxiety-Like Behaviors Through Downregulation of Dopamine and Inflammation in the Amygdala of Mice

et al. 2020

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Background: A high-fat diet (HFD) can induce obesity and metabolic disorders that are closely associated with cognitive impairments, and the progression of several psychiatric disorders such as anxiety. We have previously demonstrated the anxiolytic-like effect of Gamma oryzanol (GORZ) in chronic restraint stressed mice.Objective: We studied the neurochemical and molecular mechanisms that underlie the preventive effect of GORZ in HFD-induced anxiety-like behaviors, monoaminergic dysfunction, and inflammation.Methods: Eight-week-old Institute of Cancer (ICR) male mice weighing 33-34 g were divided into the following groups and free-fed for 8 weeks: control (14% casein, AIN 93M); HFD; HFD + GORZ (0.5% GORZ). Body weight gain was checked weekly. The anxiolyticlike effects of GORZ were examined via open-field test (OFT) and elevated plus maze (EPM) test. Brain levels of monoamines [5-hydroxy tryptamine (5-HT), dopamine (DA), and norepinephrine (NE)] and their metabolites [5-hydroxyindole acetic acid (5-HIAA), homovanillic acid (HVA), and 3-methoxy-4-hydroxyphenylglycol (MHPG)], proinflammatory cytokines such as tumor necrosis factor-aa (Tnf-a) mRNA levels, and interleukin 1-b (Il-1b) mRNA levels in the cerebral cortex and amygdala were examined using high-performance liquid chromatography-electrochemical detection (HPLC-ECD), and real-time reverse transcription-polymerase chain reaction (RT-PCR), respectively.Results: Mice fed a HFD for eight weeks showed anxiety-like behaviors in association with HFD-induced body weight gain. GORZ potentially blocked HFD-induced anxiety-like behaviors via significant improvement of the primary behavioral parameters in behavioral tests, with a minor reduction in HFD-induced body weight gain. Furthermore, GORZ treatment significantly downregulated HFD-induced upregulation of dopamine levels in the brain's amygdala. Significant reduction of the relative mRNA expression of Tnf-a and Il-1 b was also observed in the amygdala of HFD + GORZ mice, compared to HFD mice. Conclusions:Our findings strongly suggest that 0.5% GORZ exerts anxiolytic-like effects, possibly through downregulation of dopamine, and via expression of proinflammatory cytokines Tnf-a and Il-1 b in the case of chronic HFD exposure.

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Section: Discussionmentioning

confidence: 84%

Section: Treatmentsmentioning

confidence: 99%

Section: Animal Sacrifice and Sample Collectionmentioning

confidence: 99%

“…EPC-300 software (version 2.5.10, Eicom) was used to evaluate the data. The applied protocol is published in detail in our existing paper (Akter et al, 2019).…”

Section: High-performance Liquid Chromatography-electrochemical Detecmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Gamma Oryzanol Alleviates High-Fat Diet-Induced Anxiety-Like Behaviors Through Downregulation of Dopamine and Inflammation in the Amygdala of Mice

et al. 2020

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Efficacy and feasibility analysis of compound fluocinolone acetonide cream combined with guaiazulene in the treatment of neurodermatitis

Yan,

Cai,

Wang

2024

Arch Dermatol Res

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Treadmill Exercise Improves Behavioral and Neurobiological Alterations in Restraint-Stressed Rats

Ercan,

Bulmus,

Kacar

et al. 2023

J Mol Neurosci

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Stress is a state that is known to impact an organism's physiological and psychological balance as well as the morphology and functionality of certain brain areas. In the present work, chronic restraint stress (CRS) model rats treated with treadmill exercise were used to examine anomalies associated to emotion and mood as well as molecular changes in the brain. 40 male Sprague Dawley rats were divided into control, stress, exercise, and stress+exercise groups. CRS were exposed to stress group rats and exercise group underwent a chronic treadmill exercise. Depressive-like behavior was evaluated with the forced swim test(FST) and tail suspension test(TST). For assessing anxiety-like behavior, the light-dark test(LDT) and the open eld test(OFT) were used. Morris water maze test(MWMT) were used for testing memory and learning. Brain's monoamine level and the expression of genes related to stress were measured. It was discovered that CRS lengthens latency in the MWMT, increases immobility in the FST and TST, decreases time in the light compartment and causes hypoactivity in the OFT. CRS reduced the dopamine levels in the nucleus accumbens(NAc). Brain-derived neurotrophic factor (BDNF), dopamin receptors and serotonin receptor (HTR2A) gene expression in the prefrontal cortex, corpus striatum and hypothalamus were decreased by CRS. Exercise on a treadmill lead to increase NAc's dopamine and noradrenaline levels and prevented behavioral alterations. Exercise increased the alterations of BDNF expressions in the brain in addition to improving behavior. As a result, CRS-induced behavioral impairments were effectively reversed by chronic treadmill exercise with molecular alterations in the brain.

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Anxiolytic effects of γ-oryzanol in chronically- stressed mice are related to monoamine levels in the brain

Cited by 18 publications

References 44 publications

Gamma Oryzanol Alleviates High-Fat Diet-Induced Anxiety-Like Behaviors Through Downregulation of Dopamine and Inflammation in the Amygdala of Mice

Gamma Oryzanol Alleviates High-Fat Diet-Induced Anxiety-Like Behaviors Through Downregulation of Dopamine and Inflammation in the Amygdala of Mice

Efficacy and feasibility analysis of compound fluocinolone acetonide cream combined with guaiazulene in the treatment of neurodermatitis

Treadmill Exercise Improves Behavioral and Neurobiological Alterations in Restraint-Stressed Rats

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